Olfr1500 (NM_001011831) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG214717
Olfr1500 (tGFP-tagged) - Mouse olfactory receptor 1500 (Olfr1500), (10ug)
  $650.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr1500
Synonyms MOR212-4P
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214717 representing NM_001011831
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAAGGTGAATCTCACTTTGGTGACAGAGTTCCTCCTCATAGCATTCACTGAGCATCCTGAATGGG
GGCTCCCTCTTTTCCACCTGTTTTTATTTATCTATCTCTTCACTTTGCTAGGGAACTCAGGCATGATTGT
TTTGATCCGCATGGATCGCAGGCTCCACACCCCAATGTACTTCCTTCTAAGCCACCTCTCTTTCATGGAT
ATCTGTTACTCCTCTGTCACTGTTCCTCAGACAATGGCTGTGCTGTTGGAGCATGGGGCAGCTTTATCCT
ATGCACGCTGTGTTGCTCAGTTTTTCCTGTTTACTTTTTTTGGATCCATCGATTGCTACCTCTTGGCTCT
CATGGCCTACGACCGCTATGTGGCCGTGTGCCAGCCTTTGCTTTATGTCACCATAATGACACAGAAAGCC
CTTCTAAGTTTTGTGGCTGGGGCTTACATTGCTGGCCTCCTCAGTGCCTTGGTGAGGACAATCTCAGCAT
TCACCCTCTCTTTTTGTGGAAACAATGAGATCGACTTTATTTTTTGTGACCTTCCTCCTCTGTTAAAGCT
GACCTGTGGTGAGAGCTATATCCAGGAATTGGTGATTATTGTGTTTGCCATTTTTGTCATCCCTGCATGC
ATGGTGGTGATTGTGGTTTCCTACCTGTTCATCATTGTGGCCATTCTGAGGATCCCTTCAGCAGGAGGCC
GGGCCAAGACCTTCTCAACATGTGCCTCCCACCTCACTGCAGTGTCACTCTTCTTTGGCACTCTCATTTT
CATGTACCTGAGAGACAACTCTGGTCAGGCCTCAGAAAAGGATCGAGTTGTGTCTGTGTTCTACACAACA
GTAATCCCCATGTTGAATCCCCTCATCTACAGTTTGAGGAACAAGGAAGTGAAGGAAGCACTGAAAAATT
TTCTCAATAGAGTGAAGACTTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214717 representing NM_001011831
Red=Cloning site Green=Tags(s)

MAKVNLTLVTEFLLIAFTEHPEWGLPLFHLFLFIYLFTLLGNSGMIVLIRMDRRLHTPMYFLLSHLSFMD
ICYSSVTVPQTMAVLLEHGAALSYARCVAQFFLFTFFGSIDCYLLALMAYDRYVAVCQPLLYVTIMTQKA
LLSFVAGAYIAGLLSALVRTISAFTLSFCGNNEIDFIFCDLPPLLKLTCGESYIQELVIIVFAIFVIPAC
MVVIVVSYLFIIVAILRIPSAGGRAKTFSTCASHLTAVSLFFGTLIFMYLRDNSGQASEKDRVVSVFYTT
VIPMLNPLIYSLRNKEVKEALKNFLNRVKTF

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001011831
ORF Size 933 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001011831.1, NP_001011831.1
RefSeq Size 936 bp
RefSeq ORF 936 bp
Locus ID 258097
Cytogenetics 19 A
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001011831" in other vectors (4)
SKU Description Size Price
MC213377 Olfr1500 (untagged) - Mouse olfactory receptor 1500 (Olfr1500), (10ug) 10 ug
$480.00
MR214717 Olfr1500 (Myc-DDK-tagged) - Mouse olfactory receptor 1500 (Olfr1500) 10 ug
$450.00
MR214717L3 Lenti ORF clone of Olfr1500 (Myc-DDK-tagged) - Mouse olfactory receptor 1500 (Olfr1500) 10 ug
$750.00
MR214717L4 Lenti ORF clone of Olfr1500 (mGFP-tagged) - Mouse olfactory receptor 1500 (Olfr1500) 10 ug
$750.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.