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Ccdc146 (NM_029195) Mouse Tagged ORF Clone

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SKU
MG214688
Ccdc146 (tGFP-tagged) - Mouse coiled-coil domain containing 146 (Ccdc146), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,094.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccdc146
Synonyms 4930528G09Rik; mKIAA1505
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214688 representing NM_029195
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATACAATAGCAAAGATGCAGAAGAAGAAACTGAAGATGAAGAGGAGGGACTGAAGGAGGCGGAGG
GGGAGGAACAGGAGAAGGAGGAGGTAACAGCAACCTCAGAAACAGACGATGACCAAGAAGACCTCCCTTC
TGTCTTAGTGCCTACAGTTAACATTCGGGAGGAGCGGCTCATCAATTTAGCTGAAACACCAGCTTTCCTC
TGTCTGCATGAGCTGCACTCCAAGGGAAAACTTCCTGGAACCAGAATGGCAGAGTTGAAAGCCAAGTACA
CCTTGCTACACGATACAGTGGTGAGCACGCAGGAGTCTGAGGTGCAGCTACTGGAGAACGCCAAGCGCTT
CACGGAGCAGATCCAGCAGCAGCAGGTCTGTCTGCAGCAGGCCGAAGACTTCCCCAACGTCTTCACCACT
GAGGTCTGCAAACTTCGGGAGCAGCTGCTCAAGTACCAAAATGAATACACGGCAGCGCAAGAAAGGGAGT
ACAATATTCAGTACAGGCTGACTAGCTTAACAGAAGAAAAGAGTATCATATTAAAGGAATTTGAGAAGAT
ACCTAAGCCAGGAGAAATAGAGAAGAAGACGAGGCTCCTGAAAGAAAGCACTGAGGAACTGCGCAAGGAG
GTGATTCAGAGGAGACTGGAGATCAAAAACCTGCGGGAAGATGTGGTGCTCAAGCAGAAGCAGCTAGTGA
GGGAGCAGAAGGAGCTGGAGGAGCTGATGGAGTATCAGGTTGGCTTGAAGGATGACGTGGTCCACCATCA
AAGTGTTCCTGTACAAATTACAAAGGAGATAGAAAAAATGACACGCAAAAAAGTAGAAACAGAGAAGAAA
AATATTGTCTTGGAATCTGAGTTGAAAGAGCTAAGTGACTCTTTAAAGAAGCTTGAAAACAAAGTCAACA
CTCTTGCGGAAGAAAGGGATGACATAATGAAGGAAGTTGAGGGCAAGCGAACCTTACTAGAAGTCAAAGA
ACGAGAGTATGGCCAACTGCTTAAACTATTGGAACTAACCAAAGAGAACGAAGCCTCCTCGCTAGCTGAA
AGAGGGATCTTGGATATCAATCTACGGAACTGTCTCATCGACAAGCAGAACTACCATGATGAACTTTCTC
GTAAGCAAAGAGAGAAAGAACGAGATTTTCGGAATCTGAAGAAGACAGAGCTGCTCCTGAAAGTGTCTTT
GGATGCGCTCACTCAGGCACAAGCCATGAATCAAAGGCTTCTACTAGAGATGGAAGCTATCCCAAAAGAA
TACCTTACATTGCCTGAACGAAGGAAAGAGCTCCACAAAGAGGTTGATCTAGCTAGGAGAAATCTGGCTC
AACAGAAATCTCTGTCAGAAGCTGAGGCCAAGTTAGTGGAGCAGCAGATTGCACAGGAGAACAAACTTCT
AAAGGAGCAAGAAAGTTTGCGGGAGCTGGTGTTCAACCTCGGGCGAATGACTCAGATCAAAATAGATGAG
AAAGAACAAAAGGCTAAGGATTTCCTGAAAGCCCAGCGAAGATATAGTGAGATTGTTAAGGAAATCAAAT
CAAAGGACCTTGAAATCAGGCTATACAAGAAGAGAAAGCATGAAATTCATAGGAGACTCAGAGAATTTGC
CAGCTTGTATGATACAATTAGAAATGAAAGAAACAAATTTGTAAACCTACTTCACAAAGCTCATCAGAAA
GTAAATGAGATAAAAGAAAGACTCAAAATGTCATTAAATGAGCTGGAAATCTTAAGGAACAGCGCTGTCT
CTCAAGAACGAAAGCTACAAAACGCCATGCTAAAGCATTCCAACAATGTGACCATCAAGGAGAGCATACA
GAACGATGTGTGCAAAATCACTGCTAAACTCCAGGAAATGAAGGAGAAGAAGGAGGCCCAGCTGACCAGC
ATGGACAGGCTGGCCAGCATGATCACCGTGATCGAAGAGGAGATGGTGCAGCTGCGCAAGAAGTATGAGA
AGGCCGTACAGCGGCGGAATGAAAGTGGTGTTCAGCTGATCGAGCGGGAAGAAGAAGTGTGCATTTTTTA
CGAAAAAGTGAATGTCCAAGAGAAGATAAAACTTCATCAGGATGTTGAAATCCACATTCTGGAAGAAAAA
ATCCGATTCCTAAAACTGAGGATTGCCGAAAAGCAAAGGCAGATTTCTGTGACACGGAAACTAGTGCCAA
TCAAGAAGTCCCTGGATGCCGACCTGGCTGTGATCCAGATTCAGTTTTCACAGTGCACAGACAGGATAAA
AGACCTGGAGAAACTCTTTGTGAACCCTGACAGCAAGGGCAGGGTCCGCTTCATTAAAGGGAAAGATCTG
ACTGAAGAAGAAATGATCAAGAAATTAGATATGCTGGAACTTCAGATAGCCAAGAAGGAGGAGAAGCTCC
TGGAGAAGGATTTCATCTATGAGCAGGTGTCCCAGCTCACAAACAGGCTCAAAGGCAAAACTCAGGCTTG
CAAGAAGGACACACTGCTCTTAGCCAAGAAGATGAATAGCTACCAACAAAAGATCAAAGTTGTTACCCAG
GAAATGATGGCTCTTGTTGCTGAGCTGTCCATGAAACAAGCCCTGACCATTGAACTCCAAAAGGAGGTGA
GAGAGAAAGAAGAATTCATCTTCTCCTGCAGTGCCAGGATAGAAAAGGGCCTCCCGCTCAACAGAGAGAT
TGAGAAGGACTGGCTGAAGGTGCTTCGAGATGAAGAAATGTATGCCTTTGCGACTGCTGAGATGTCTCGG
GAGTACATGGAAACGGATTATCGGCAGCTGCCCAATGGTGTTTATACAACTGCAGAGCAGCGTCCAAACG
CCTACATGCCTGAAGCAGATACCGAGCTTCCTCTGCCCAAACCGTATGGCGCGATGGCTCCATTTAAGCC
CTCTGAACCCGGAGCCAACAGGAGGCACATTAGGAAACCTGTTGTAAAGCCCATTGAAATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214688 representing NM_029195
Red=Cloning site Green=Tags(s)

MEYNSKDAEEETEDEEEGLKEAEGEEQEKEEVTATSETDDDQEDLPSVLVPTVNIREERLINLAETPAFL
CLHELHSKGKLPGTRMAELKAKYTLLHDTVVSTQESEVQLLENAKRFTEQIQQQQVCLQQAEDFPNVFTT
EVCKLREQLLKYQNEYTAAQEREYNIQYRLTSLTEEKSIILKEFEKIPKPGEIEKKTRLLKESTEELRKE
VIQRRLEIKNLREDVVLKQKQLVREQKELEELMEYQVGLKDDVVHHQSVPVQITKEIEKMTRKKVETEKK
NIVLESELKELSDSLKKLENKVNTLAEERDDIMKEVEGKRTLLEVKEREYGQLLKLLELTKENEASSLAE
RGILDINLRNCLIDKQNYHDELSRKQREKERDFRNLKKTELLLKVSLDALTQAQAMNQRLLLEMEAIPKE
YLTLPERRKELHKEVDLARRNLAQQKSLSEAEAKLVEQQIAQENKLLKEQESLRELVFNLGRMTQIKIDE
KEQKAKDFLKAQRRYSEIVKEIKSKDLEIRLYKKRKHEIHRRLREFASLYDTIRNERNKFVNLLHKAHQK
VNEIKERLKMSLNELEILRNSAVSQERKLQNAMLKHSNNVTIKESIQNDVCKITAKLQEMKEKKEAQLTS
MDRLASMITVIEEEMVQLRKKYEKAVQRRNESGVQLIEREEEVCIFYEKVNVQEKIKLHQDVEIHILEEK
IRFLKLRIAEKQRQISVTRKLVPIKKSLDADLAVIQIQFSQCTDRIKDLEKLFVNPDSKGRVRFIKGKDL
TEEEMIKKLDMLELQIAKKEEKLLEKDFIYEQVSQLTNRLKGKTQACKKDTLLLAKKMNSYQQKIKVVTQ
EMMALVAELSMKQALTIELQKEVREKEEFIFSCSARIEKGLPLNREIEKDWLKVLRDEEMYAFATAEMSR
EYMETDYRQLPNGVYTTAEQRPNAYMPEADTELPLPKPYGAMAPFKPSEPGANRRHIRKPVVKPIEI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029195
ORF Size 2931 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029195.1, NP_083471.1
RefSeq Size 3389 bp
RefSeq ORF 2934 bp
Locus ID 75172
Cytogenetics 5 A3
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Documents
Resources
"NM_029195" in other vectors (4)
SKU Description Size Price
MC222911 Ccdc146 (untagged) - Mouse coiled-coil domain containing 146 (Ccdc146), (10ug) 10 ug
$985.00
MR214688 Ccdc146 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 146 (Ccdc146) 10 ug
$589.00 $894.00
MR214688L3 Lenti ORF clone of Ccdc146 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 146 (Ccdc146) 10 ug
$1,194.00
MR214688L4 Lenti ORF clone of Ccdc146 (mGFP-tagged) - Mouse coiled-coil domain containing 146 (Ccdc146) 10 ug
$1,194.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.