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Dpy19l2 (NM_001166207) Mouse Tagged ORF Clone

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SKU
MG214684
Dpy19l2 (tGFP-tagged) - Mouse dpy-19-like 2 (C. elegans) (Dpy19l2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $908.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dpy19l2
Synonyms 4932443J21Rik; Gm18376
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214684 representing NM_001166207
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGGGCCGACAAGGAGTAAGCTCAGGGAAGGGAGCTCCGACAGGCCACAATCCTCCTGCACGGGGC
AGGCGAGGAGGAGGTGGAGCGCAGCGACCATGGAGCCCCAGCAGGAGCGAAGCGCGCCCCAGGAGAGAAC
GAAGTGGTCGTTGCTGCAGCATTTCCTCCTAGGCGGCAGGAAACTGCCCTCGGGTGCTCGCAACTACGCG
GCGCGGAGGATCCAAAGTCTGAACGCACAAAATTACTTTCAGCTGGAAGAGGTCGCCAAGCTCCTTCTTC
TTAACCGCTTCCAATTCCTCTTCACTCTCCTGGACCACTTTCGCGAAAAGGTGCAGGCACTGCAGATGCA
TCGGTTCTCCCACAGGACCCTCTTTGGCCTAGCTATCTTTGTGGGGATTTTGCACTGGTTACACTTAATA
ACACTTTTTGAAAATGATCATCATTTTTCTCACCTTTCATCTTTGGAACGGGAGATGACTTTTCGCACAG
AAATGGGACTTTATTATTCCTACTTCAAGACCATTATCGAAGCACCTTCGTTTCTGGAAGGACTGTGGAT
GATCATGAATGACAGGCTCACTGAGTATCCCCTGGTAATCAACACAGTGAAACGCTTCCATCTTTACCCA
GAGGTAGTCATAGCCTACTGGTATCGCACAATCATAGGAATAATGAATTTATTTGGGATAGAAACTAAGA
CATGTTGGAACGTCACCAGAATGGAGCCTCTTAATGAAGTTCAGAGCTGTGAAGGATTGGGAGACCCAGC
TTGCTTTTACATCGGAGTAATTTTTATTTTAAATGGACTAATGATGGGCTTGTTCTTCATATACAGTACA
TACCTGAGTGGGAGTCAGTTAGGAGGTCTGATTACAGTAGCCTGCTACTTTTTCAATCATGGAGAGGCCA
CCCGTGTGATGTGGACACCGCCTCTCCGGGAAAGTTTCTCCTACCCATTCCTTGTACTTCAGATGTACAT
CTTAACTATAATCCTCAGGACCTCCACCGTTCACAAAAAACACTACATGGCTCTCTGCTTCTCCAACGTC
GCTTTTATGCTTCCGTGGCAGTTTGCTCAGTTTATACTTTTTACACAGATAGCCTCATTATTTCCCATGT
ATGTTGTGGGATACATTGAGCCAAGCAAATTTCAGAAGATCATTTATGTCAATATGAGTTCGGTTGCCCT
ATGCTTCATTTTGATGTTTGGGAATTCAATGTACTTAAGTTCATATTATTCCTCATGTTTGTTAGTGACA
TGGGCAATAATGCAAAAGAAAAGCAAAATCCAAAAATTGGGAGGCACTGAACTTCAGTTTTGGCTAATCC
AAGGCTGTTTTTGGTGGTGTGGAACAATTATTTTGAAATTTCTGACATCTAAAATCTGTGGTGTTTCAGA
CCATATTCGCCTGAGTGACCTTATAGCAGCCAGAATACTAAGGTATACAGATTTTGATACCTTAATATAC
ACCTGTGCTCCCGAATTTGACTTCATGGAACAAGCGACTCCGCTGAGATATATAAAAACATTATTGCTTC
CACTTATCCTAGTGATTACTTATCTTATCTTTAAAAAGATTGTTCGTGATATCATGTGTGTCTTATATAC
AAACACTTATGTAAGAAAACAGCTTCTTGACAACGCTGAGTTGATTTTCCACACCTTGCAGTTGCTGGCA
TTCACAGGTCTTGCCATCTTAATCATGCGGTTGAAGCTGTTTCTGACCCCCCACATGTGCATTATGGCTT
CCTTGATCTGCTCACAACGGCTCTTTGGCTGGCTTTTTTGCAGAATTCATTTTGAGAATGTTGTCTTCGG
CATTCTGACAATGATGTCAATACAAGGTTGTGCAAATCTCCATAACCAATGGAGCATAATGGGAGAATTT
ACTAACTTGCCTCAGGAAGAGCTCATACATTGGATCAAACACAGCACCAGGCCAGATGCCGTCTTTGCAG
GTGCCATGCCTACAATGGCAAGCATCAAGCTGTCTACACTGCGTCCAATTGTGAACCACCCACACTATGA
GGACGCAGACTTGAGGGCTCGGACAAAAATAGTTTATTCTGTGTATAGTCGAAAATCTGCAGTAGAAGTG
AGAAATAATCTGTTGAAATTACATGTGAATTATTATGTTCTAGAAGAGGCATGGTGTGTCGTGAGAACAA
AACCTGGTTGCAGCATGCTTGAAATATGGGATGTGGAAGACCCGTCCAACGCAGCTAACCCTCCCCTCTG
CAGTATCCTGCTCAAGGATTCTCGGCCGTACTTTACCACAGTGTTTCAGAATAGTATGTACAGAGTACTG
AAGATTAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214684 representing NM_001166207
Red=Cloning site Green=Tags(s)

MVGPTRSKLREGSSDRPQSSCTGQARRRWSAATMEPQQERSAPQERTKWSLLQHFLLGGRKLPSGARNYA
ARRIQSLNAQNYFQLEEVAKLLLLNRFQFLFTLLDHFREKVQALQMHRFSHRTLFGLAIFVGILHWLHLI
TLFENDHHFSHLSSLEREMTFRTEMGLYYSYFKTIIEAPSFLEGLWMIMNDRLTEYPLVINTVKRFHLYP
EVVIAYWYRTIIGIMNLFGIETKTCWNVTRMEPLNEVQSCEGLGDPACFYIGVIFILNGLMMGLFFIYST
YLSGSQLGGLITVACYFFNHGEATRVMWTPPLRESFSYPFLVLQMYILTIILRTSTVHKKHYMALCFSNV
AFMLPWQFAQFILFTQIASLFPMYVVGYIEPSKFQKIIYVNMSSVALCFILMFGNSMYLSSYYSSCLLVT
WAIMQKKSKIQKLGGTELQFWLIQGCFWWCGTIILKFLTSKICGVSDHIRLSDLIAARILRYTDFDTLIY
TCAPEFDFMEQATPLRYIKTLLLPLILVITYLIFKKIVRDIMCVLYTNTYVRKQLLDNAELIFHTLQLLA
FTGLAILIMRLKLFLTPHMCIMASLICSQRLFGWLFCRIHFENVVFGILTMMSIQGCANLHNQWSIMGEF
TNLPQEELIHWIKHSTRPDAVFAGAMPTMASIKLSTLRPIVNHPHYEDADLRARTKIVYSVYSRKSAVEV
RNNLLKLHVNYYVLEEAWCVVRTKPGCSMLEIWDVEDPSNAANPPLCSILLKDSRPYFTTVFQNSMYRVL
KIN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001166207
ORF Size 2319 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166207.1, NP_001159679.1
RefSeq Size 3441 bp
RefSeq ORF 2322 bp
Locus ID 320752
UniProt ID P0CW70
Cytogenetics 9 A4
Summary Probable C-mannosyltransferase that mediates C-mannosylation of tryptophan residues on target proteins. Required during spermatogenesis for sperm head elongation and acrosome formation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001166207" in other vectors (4)
SKU Description Size Price
MC221541 Dpy19l2 (untagged) - Mouse dpy-19-like 2 (C. elegans) (Dpy19l2), (10ug) 10 ug
$780.00
MR214684 Dpy19l2 (Myc-DDK-tagged) - Mouse dpy-19-like 2 (C. elegans) (Dpy19l2) 10 ug
$589.00 $708.00
MR214684L3 Lenti ORF clone of Dpy19l2 (Myc-DDK-tagged) - Mouse dpy-19-like 2 (C. elegans) (Dpy19l2) 10 ug
$1,008.00
MR214684L4 Lenti ORF clone of Dpy19l2 (mGFP-tagged) - Mouse dpy-19-like 2 (C. elegans) (Dpy19l2) 10 ug
$1,008.00

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