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Gm14139 (NM_001145863) Mouse Tagged ORF Clone

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SKU
MG214636
Gm14139 (tGFP-tagged) - Mouse predicted gene 14139 (Gm14139), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $703.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm14139
Synonyms OTTMUSG00000015769
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214636 representing NM_001145863, codon optimized.
Due to the complexity of NM_001145863, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCGAGTCACCTACGACGACGTGCACGTGAATTTTACTAGGGAAGAGTGGGCTCTCTTGGATAGCA
GCCAGAAAAACCTGTATAAAGAGGTAATGCTCGAAACATATCGCAATCTCGCTGCAATTGGTTACGTGTG
GGAGGACCACAATACCGAGGAGCATCTCCACATCTCTCGGAGATACGGACGCCACGAACGAAGCCACACC
GAGCAGCTCCTGAGCGAGTTTATCCAGTGCGATAAGGCTTTCGCTTTCCAGAGCCACAATCAAAGGCAAG
AGAGGATTCACACCGCAGAAACCCCATACGAATGCAACCAATGTGGAAAATCCTTCGCTAGGAAGTCCCA
TTTGCTCAGGCACAAGCGGACACACACCGGCGAGAAACCCTTTCAGTGCTACCAGTGTGGAAAGACCCTC
GCCCGAAACACCCACCTCCTTCAGCACAAGAAGGTTCACACAGGTGAAAAGCCTTATGAATGCAAACATT
GTGATAAAGCTTCCGTTTGTCATATCGGATTGCTCTACCATAAAAGAACCCACGCCGGGCAGAGGCCTTA
TGAATGGAATGAGTGTGGAAAGAGTATTGTGTATCACAGCTGCATCAAACAGTGCAAACGGAGACACACA
GGTGAGAAGCCTTTTGAGTGCAACCAGTGTGGCAAGTCTTTCGCACGCAAAAGCAACCTGCTCAGGCATA
AGCGGATCCATACTGGGGAGAAACCTTACAAATGCGACCAGTGTGGGAAGGATTTCGCTTCTCCCAGCTC
TCTGAAGTGTCATAAAAGAGTGCACACCGGCGAGAACCCTTACGAGTGCAAACAGTGTGGCAAGGCATTT
GCACGCCTCTCCATATTGAGGACCCACAAGCGCACCCACACGGGCGAGAAGCCCTTTGAGTGTAACGAGT
GTGGCAAGAGCTTCGCTCGAAAGTCTCACCTCCTGCGACATAAGAGGATACACACCGGCGAAAAGCCTTA
CAAGTGCGACCAGTGTGGCAAAGATCTGGCCTGCCCAAATAGCCTCCAGTATCATAAAAGGGTGCATACC
GGAGAGAAGAGCTACGAATGTAAGCAATGTGGCAAGGCCTTCGCCTTTTCCACTTCCTTGCGCATCCATA
AGCGAACTCACACAGGGGAAAAGCCTTACGAGTGCAATCAGTGCGGGAAAGCTTTCAGTTCTCCCAGCGG
ATTTCTGTATCACAAGCAGACCCATACCGGCGAACGCCCCTACGAGTGCAACGAGTGCGGGAAGAGTTTT
ATCCACAGATCAAATTTCCAGATGCACAAGAGGACTCACACTGGGGAGAAGCCTTTCGAGTGTGATCAGT
GTGGCAAATCATTCGCTCAAAATACGTACCTCCTGCAGCACAAGCGGGTGCATACGGGCGAAAAGCCTTA
CGAGTGCAAGCACTGCGGGAAAGCCTTTGCTTACAGTAATAATCTCAGAGTGCACATTCGCACGCATACA
AAATATCTGGTGTGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214636 representing NM_001145863
Red=Cloning site Green=Tags(s)

MGRVTYDDVHVNFTREEWALLDSSQKNLYKEVMLETYRNLAAIGYVWEDHNTEEHLHISRRYGRHERSHT
EQLLSEFIQCDKAFAFQSHNQRQERIHTAETPYECNQCGKSFARKSHLLRHKRTHTGEKPFQCYQCGKTL
ARNTHLLQHKKVHTGEKPYECKHCDKASVCHIGLLYHKRTHAGQRPYEWNECGKSIVYHSCIKQCKRRHT
GEKPFECNQCGKSFARKSNLLRHKRIHTGEKPYKCDQCGKDFASPSSLKCHKRVHTGENPYECKQCGKAF
ARLSILRTHKRTHTGEKPFECNECGKSFARKSHLLRHKRIHTGEKPYKCDQCGKDLACPNSLQYHKRVHT
GEKSYECKQCGKAFAFSTSLRIHKRTHTGEKPYECNQCGKAFSSPSGFLYHKQTHTGERPYECNECGKSF
IHRSNFQMHKRTHTGEKPFECDQCGKSFAQNTYLLQHKRVHTGEKPYECKHCGKAFAYSNNLRVHIRTHT
KYLVW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001145863
ORF Size 1485 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145863.1, NP_001139335.1
RefSeq Size 1728 bp
RefSeq ORF 1488 bp
Locus ID 100271882
Cytogenetics 2 G3
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Documents
Resources
"NM_001145863" in other vectors (2)
SKU Description Size Price
MC217113 Gm14139 (untagged) - Mouse predicted gene 14139 (Gm14139), (10ug) 10 ug
$503.00
MR214636 Gm14139 (Myc-DDK-tagged) - Mouse predicted gene 14139 (Gm14139). Note: ORF is codon optimized 10 ug
$503.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.