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Gm14124 (NM_001142410) Mouse Tagged ORF Clone

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SKU
MG214628
Gm14124 (tGFP-tagged) - Mouse predicted gene 14124 (Gm14124), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $920.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm14124
Synonyms EG640962; ENSMUSG00000074735; Gm10749; OTTMUSG00000015734
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214628 representing NM_001142410, codon optimized.
Due to the complexity of NM_001142410, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCGAGTTACTTACGATGATGTGCATGTTAACTTCACCAGGGAGGAATGGGCTCTCCTTGACCCCA
GTCAGAAGAACCTGTATAGGGACGTAATGCTGGAGACATACCGAAACTTGGCCGCCATTGGATATGTGTG
GGAGGATCATAATACTGAAGAACATTTGCACATATCTCGCAGGTATGAGCGGCATGAACGGAGCCACACT
GCACAACAGCTCTCCGAGTTCATCCAGTGTGACAAGGCTTTTGCTTTCCAGAGCCACAACCAAAGAAAGG
AGAAGATACATAATGCTGAGACGCCCTATGAGTGTAACCAGTGCGGGAAATCCTTTGCGAGAAAATCACA
CCTGCTTCGCCACAAGAGAATTCACACCGGGGAAAAGCCCTACGCCTGTAATCAGTGCGGTAAAGATTTC
GCCTCACCTTCCTACTTGCAGTATCACAAGCGAGTGAGAACGGGTGAAAAACCATACAAATGCAACCAGT
GCGGAAAAGCTTTTGCGTGTTCCACCGGGCTGCGGGGTCATAAGCGGACACACACTGAAGAGAAGCTTAA
TGAGTGCAATCAGTGCGATAAGGCATTCGCCGGACAAAGTGGACTGCTCTACCACAAACGCACACACACC
GGGGAGAAGCCATTCCAGTGTAATCAGTGTGGTAAGACCTTCGCTAGAAATACACACCTCTTGCAGCACA
AAAGGGTCCACACGGGTGAAAAGCCCTATGAGTGCAACCAATGTGAAAAAGCATCCGTGTGTCATATCGG
GCTGCTGTATCACAAGAGGACTCACGCAGGTCAGCGGCCTTACGAGTGCAACGAGTGTGGCAAAAGTATA
GTGTACCATAGCTGCATCAAGCAATGCAAAAGGAGGCACACAGGGGAAAAGCCCTTCGAGTGCAATCAGT
GCGGAAAGAGCTTCGCCAGAAAAAGCCATCTGCTGCGACACAAGCGGATCCACACTGGTGAAAAGCCATA
CAAGTGCGATCAGTGCGGCAAGGACTTCGCCAGGCCAAGTTCCTTGCAGTATCATAAGAGGGTTCATACC
GGTGAGAAGCCTTACGAATGTAAGCAGTGTGGTAAGGCATTTGCATGCTCAACTGTGCTTCGGAGACACA
AGAGAGCCCACACAGGGGAAAAACCCTTCGAGTGCTACCAGTGCGGTAAAAGTTTTGCCAGTAAAAGCCA
TCTCCTGAGACACAAGAGGATCCACACAGGGGAAAAGCCATACAAGTGCGACCAATGCGGGAAGGACTTT
GTGTATCATTCATGCATCAAGCAGTGTAAACGACGGCACGCGGGCGAAAAACCTTTTGAATGCAATCAGT
GTGGCAAGAGCTTCGCTTCTAAGTCATACCTGTTGCGCCATAAGCGCATTCACACCGGTGAAAAGCATTA
TAAGTGTGACCAGTGTGGTAAGGATTTCGCGTCTACCTACTCTCTCCAGAATCATAAAAGGGTCCACACG
GGAGAGAAACCCTACGAGTGCAAGCAGTGCGGGAAAGCTTTCGCCTGCTCTTCTAAACTGAGGAGCCATA
AGAGGACTCACACTGGCGAGAAGCCATTTGAATGCAACCAGTGCGGGAAGTCATTCGCTATTAAGTCTCA
CCTCCTCCGACACAAACGGATCCATACGGGTGAGAAGCCATACAAGTGTGATCAATGCGGCAAGGATTTC
GCCCGCCCTTCCAGCCTCCAGTATCACAAACGGGTGCATACCGGGGAGAAGCCGTACGAATGCAAGCAGT
GCGGTAAGGCGTTCGCCTGTTCAAGAAGATTGAGGATACATAAGCGGACCCACACGGGAGAGAAGCCCTA
CGAATGTAATCAGTGCGGTAAAGCCTTTAGTTCACCCTCCGGATTTCTTTACCACAAGAGGACACATACA
GGTGAGAGGCCATACGAGTGTAATGAATGTGGTAAGTCTTTCATCCACCGGAGTAATTTCCAGATGCATA
AAAGAACTCACACAGGGGAAAAGCCTTTTGAGTGCGATCAGTGTGGCAAGAGCTTCGCCCAGAACACATA
CCTTCTTCAACACAAAAGAGTACATACTGGGGAAAAGCCATACGAGTGTAAGCATTGCGGCAAAGCCTTC
GCTTATTCCAACAAACTCCGAGTGCATGTTCGCACTCACACTAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214628 representing NM_001142410
Red=Cloning site Green=Tags(s)

MGRVTYDDVHVNFTREEWALLDPSQKNLYRDVMLETYRNLAAIGYVWEDHNTEEHLHISRRYERHERSHT
AQQLSEFIQCDKAFAFQSHNQRKEKIHNAETPYECNQCGKSFARKSHLLRHKRIHTGEKPYACNQCGKDF
ASPSYLQYHKRVRTGEKPYKCNQCGKAFACSTGLRGHKRTHTEEKLNECNQCDKAFAGQSGLLYHKRTHT
GEKPFQCNQCGKTFARNTHLLQHKRVHTGEKPYECNQCEKASVCHIGLLYHKRTHAGQRPYECNECGKSI
VYHSCIKQCKRRHTGEKPFECNQCGKSFARKSHLLRHKRIHTGEKPYKCDQCGKDFARPSSLQYHKRVHT
GEKPYECKQCGKAFACSTVLRRHKRAHTGEKPFECYQCGKSFASKSHLLRHKRIHTGEKPYKCDQCGKDF
VYHSCIKQCKRRHAGEKPFECNQCGKSFASKSYLLRHKRIHTGEKHYKCDQCGKDFASTYSLQNHKRVHT
GEKPYECKQCGKAFACSSKLRSHKRTHTGEKPFECNQCGKSFAIKSHLLRHKRIHTGEKPYKCDQCGKDF
ARPSSLQYHKRVHTGEKPYECKQCGKAFACSRRLRIHKRTHTGEKPYECNQCGKAFSSPSGFLYHKRTHT
GERPYECNECGKSFIHRSNFQMHKRTHTGEKPFECDQCGKSFAQNTYLLQHKRVHTGEKPYECKHCGKAF
AYSNKLRVHVRTHTK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001142410
ORF Size 2145 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001142410.1, NP_001135882.1
RefSeq Size 3018 bp
RefSeq ORF 2148 bp
Locus ID 100216455
Cytogenetics 2 G3
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Documents
Resources
"NM_001142410" in other vectors (2)
SKU Description Size Price
MC221064 Gm14124 (untagged) - Mouse predicted gene 14124 (Gm14124), (10ug) 10 ug
$721.00
MR214628 Gm14124 (Myc-DDK-tagged) - Mouse predicted gene 14124 (Gm14124). Note: ORF is codon optimized 10 ug
$720.00

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