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Majin (NM_001165919) Mouse Tagged ORF Clone

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SKU
MG214597
1700123I01Rik (tGFP-tagged) - Mouse RIKEN cDNA 1700123I01 gene (1700123I01Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Majin
Synonyms 1700123I01Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214597 representing NM_001165919
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTTAAAACCATTCACCTACCCATTTCCAGAGACAAGGTTCCTTCATGCAGGACCCAATGTGTATA
AGTTCAAAATCAGATATGGCAACAGTATCAGAGGAGAAGAAATAGAGGATAAGGAAGTCATCGTCCAGGA
GCTGGAGGATTCTATCCGTGCAGTCTTGGCAAACATGGACAGCCTGCAACCCTTTGTTACAGAACACTTC
ATTGTATTTCCCTATAAAAGCAAGTGGGAGAGAGTTTCCCACCTGAAGTTTAAACATGGGGAAAGCATCC
TGACGCCCTACCCGTTTGTTTTTACTTTGTATATAGAGATGAAATGGTTTGCTGAAGACTTGCCTTCCGG
GAAACCAGCAGATGATATTCCTCTTGAGTTGGTCCTAGCTGAGACGGAAGCAGAAGAAGCCACAATGAGG
AAATGGAAACGGAAACTCATGGAGGAGCCCAGCTCCCCTAGCAGGCAGGGGCCACACAGGGCCAAGATGG
AGACTTCTAGTGAAGCTTCCAGCAACAAGAAGCCTCTGAAGGAGAGCAAGAGGAGCAGGGACGAAGAAGC
CCAGCAGGAATATCAGGACACCCCAGCTTCTAATGCCATTGCTGTCAAGGAACAGGATGCTGCATTGGGG
CATGGCCTGCAAGGGTTGGTTGTCCCTCCACTGCAACATAGCAGTCCACCACCCCTGAAAGAGCCAGGAG
CCAGAGGCTTCTTGGGGTTTCTAAGTGCTCTCTTCCCGTTCCGGTACTTCTTCAAGAAGAGCGGTCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214597 representing NM_001165919
Red=Cloning site Green=Tags(s)

MSLKPFTYPFPETRFLHAGPNVYKFKIRYGNSIRGEEIEDKEVIVQELEDSIRAVLANMDSLQPFVTEHF
IVFPYKSKWERVSHLKFKHGESILTPYPFVFTLYIEMKWFAEDLPSGKPADDIPLELVLAETEAEEATMR
KWKRKLMEEPSSPSRQGPHRAKMETSSEASSNKKPLKESKRSRDEEAQQEYQDTPASNAIAVKEQDAALG
HGLQGLVVPPLQHSSPPPLKEPGARGFLGFLSALFPFRYFFKKSGQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001165919
ORF Size 768 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001165919.1, NP_001159391.1
RefSeq Size 1613 bp
RefSeq ORF 771 bp
Locus ID 622554
UniProt ID Q9D992
Cytogenetics 19 A
Summary Meiosis-specific telomere-associated protein involved in meiotic telomere attachment to the nucleus inner membrane, a crucial step for homologous pairing and synapsis. Component of the MAJIN-TERB1-TERB2 complex, which promotes telomere cap exchange by mediating attachment of telomeric DNA to the inner nuclear membrane and replacement of the protective cap of telomeric chromosomes: in early meiosis, the MAJIN-TERB1-TERB2 complex associates with telomeric DNA and the shelterin/telosome complex. During prophase, the complex matures and promotes release of the shelterin/telosome complex from telomeric DNA. In the complex, MAJIN acts as the anchoring subunit to the nucleus inner membrane. MAJIN shows DNA-binding activity, possibly for the stabilization of telomere attachment on the nucleus inner membrane.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001165919" in other vectors (4)
SKU Description Size Price
MC215216 1700123I01Rik (untagged) - Mouse RIKEN cDNA 1700123I01 gene (1700123I01Rik), (10ug) 10 ug
$330.00
MR214597 1700123I01Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700123I01 gene (1700123I01Rik) 10 ug
$289.00 $300.00
MR214597L3 Lenti ORF clone of 1700123I01Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700123I01 gene (1700123I01Rik) 10 ug
$600.00
MR214597L4 Lenti ORF clone of 1700123I01Rik (mGFP-tagged) - Mouse RIKEN cDNA 1700123I01 gene (1700123I01Rik) 10 ug
$600.00

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