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Fbxw19 (NM_177703) Mouse Tagged ORF Clone

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SKU
MG214572
Fbxw19 (tGFP-tagged) - Mouse F-box and WD-40 domain protein 19 (Fbxw19), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbxw19
Synonyms E330023O18
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214572 representing NM_177703
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATCCATCTACCTAGTTTGCCTATGATGAAAATCCTCTCCTATCTGGATGCTTACAGTTTGCTAC
AGGCTGCCAAAGTGAACAAGAACTGGAATGAACTTGCAAGCAGTGATGTCCTGTGGAGGAAGTTGTGTCA
GAAGAGATGGCTCTACTGTGACAGGGTCACCCTACAGCTCCATGGCTTAGAGACATGGAAGCAGTTCTTC
ATTTCCAGAACATGGCAAGAACACGCCAAGACCCGGGCAAAGCCAGAAGATTTCAATTACAAAGAAATTC
CTGTGGCATTTGAATTTCGGGCACATCCATGTTATATCTCAAGGCATGGCTTAATAAGAAATGGACAAGG
GAAGTCTGCTGTCTGTATGGTGACTTCCACGAATAGGATTTCGACCTGGGATGTTCACGAGGGTGCTATG
ACCTGGGTAAGCCCAGTACAACCCTCCTATATCACACGGATGACTACCCTCCCTGAGATGCACATTGCAG
TCACTATAGATATGCAATCAAATATCAAACTGTGGGACTGCCACAACAGGAAAGTTTTGGCGACTACGGG
CCTGTTGTCTTCCTGTCAATTACTACAAGCTGTCTTTACAAATGATAGCCCAATTGTCTTGGTAGGTGAT
ATCTTGGGAAACCTCTACATCTTTAGGATTCCTGACTTACACCTCATTTCCAAGGTGAATGTATTCCCAT
ACGGTATTGATGAACTTCACTGCTCTCCTCAGAAGAAATGGGTCCTTCTGATAGGGAAACAAAGACATGT
CTTGACAAAGGTGTTTTACATGAGCAGCTTACTGAGGACATCAGAATTCTCTGCCCCTGTGTCTACCGAT
CTCAAATTTTCATTGTGCCAAAGAGCCTTCTGGACTCCAAGAAGGGAAGACAGGATAACCTTGATGTCCA
GTACTATACCCCCAGACCCCACAAAATTTGCCACATTTGATATGAAGCTGGAAGAGATCGAGAACAAAGT
AACTATTCAAGGACATTTGGTAGCTAGTTTCTCTTTGCAAGACTGCAAGGAGCGCGCAGAATGGATGGGA
GTCAGTGATAAGGATGTGATTGTTTGTTCAACTGGATCCTCTCTCTTGCTCTTCGACATAAATGGTCTCC
GTCTGCAGACATTTCAGTACTGCCCAGAAGAAATCTTGAGACTCTGTGTGGACCCTGTTCATGTCATTGT
CACCTGTAACAATGGCTCTTTGGATGTGTACGTGTGGGAGGAGAGAAGCCCACTGCTTAGGAGGTGTTAT
CGACTACGAAAAAGGGGATATCTGCCACTGTCTGGCTTCATTATCAAAACATTATGTGATGAATCAAGCA
TAATTTTAGTGATGACTAGTAGTCCCATTCCCTGCTTTCTGATGGCATATACCTTGAAAGTCTGCTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214572 representing NM_177703
Red=Cloning site Green=Tags(s)

MEIHLPSLPMMKILSYLDAYSLLQAAKVNKNWNELASSDVLWRKLCQKRWLYCDRVTLQLHGLETWKQFF
ISRTWQEHAKTRAKPEDFNYKEIPVAFEFRAHPCYISRHGLIRNGQGKSAVCMVTSTNRISTWDVHEGAM
TWVSPVQPSYITRMTTLPEMHIAVTIDMQSNIKLWDCHNRKVLATTGLLSSCQLLQAVFTNDSPIVLVGD
ILGNLYIFRIPDLHLISKVNVFPYGIDELHCSPQKKWVLLIGKQRHVLTKVFYMSSLLRTSEFSAPVSTD
LKFSLCQRAFWTPRREDRITLMSSTIPPDPTKFATFDMKLEEIENKVTIQGHLVASFSLQDCKERAEWMG
VSDKDVIVCSTGSSLLLFDINGLRLQTFQYCPEEILRLCVDPVHVIVTCNNGSLDVYVWEERSPLLRRCY
RLRKRGYLPLSGFIIKTLCDESSIILVMTSSPIPCFLMAYTLKVCS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177703
ORF Size 1398 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177703.3, NP_808371.1
RefSeq Size 1462 bp
RefSeq ORF 1401 bp
Locus ID 235612
Cytogenetics 9 F2
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Documents
Resources
"NM_177703" in other vectors (4)
SKU Description Size Price
MC216372 Fbxw19 (untagged) - Mouse F-box and WD-40 domain protein 19 (Fbxw19), (10ug) 10 ug
$503.00
MR214572 Fbxw19 (Myc-DDK-tagged) - Mouse F-box and WD-40 domain protein 19 (Fbxw19) 10 ug
$289.00 $457.00
MR214572L3 Lenti ORF clone of Fbxw19 (Myc-DDK-tagged) - Mouse F-box and WD-40 domain protein 19 (Fbxw19) 10 ug
$757.00
MR214572L4 Lenti ORF clone of Fbxw19 (mGFP-tagged) - Mouse F-box and WD-40 domain protein 19 (Fbxw19) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.