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Cklf (NM_029295) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214563
Cklf (tGFP-tagged) - Mouse chemokine-like factor (Cklf) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Cklf or NM_029295"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cklf
Synonyms 1700001C14Rik; 1810018M11Rik; A730028G07Rik; C32; CKLF1; Cklf2; CKLF3; CKLF4; CKLF5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214563 representing NM_029295
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACTCCACGGCCGGTCGTAAGCCGTCGGCCCTTCTGCTGTACTCTGAAATGCTTTGTGAAGTTCC
TGCGGTTGGTGGTAACTGTGACATCTATGATCTTTTTCATCGTGGGACAAGCCCCTGAACCATATATCGT
CATCACTGGGTTTGAAGTCACCGTTATTTTCTGTTTCCTAGTCTTATACACGTGTGGACTTGACAAGATA
ATGAGATCTTTCTTTTGGCCTTTGCTTGATGTTATTAACTCAATGGTAACAGCATTATGCATGCTCATTG
TGTCTGTGTTGGCTCTAATACCAGAAACCTCAACAAAGACAATCCTTGGAGGGGTGTTTGGTTTCCTGAC
AGTAACATGTACTATTGCCGACTGTGCCCTTATGTGCCAGAAACTTCGGTTTCGTCCACGTCAACCTTAT
CAGAAAAAGTCTACTAATGACATCGATGACAGAGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214563 representing NM_029295
Red=Cloning site Green=Tags(s)

METPRPVVSRRPFCCTLKCFVKFLRLVVTVTSMIFFIVGQAPEPYIVITGFEVTVIFCFLVLYTCGLDKI
MRSFFWPLLDVINSMVTALCMLIVSVLALIPETSTKTILGGVFGFLTVTCTIADCALMCQKLRFRPRQPY
QKKSTNDIDDRE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029295
ORF Size 456 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029295.3, NP_083571.1
RefSeq Size 621 bp
RefSeq ORF 459 bp
Locus ID 75458
UniProt ID Q9DAS1
Cytogenetics 8 D3
Summary May play an important role in inflammation and regeneration of skeletal muscle.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029295" in other vectors (4)
SKU Description Size Price
MC211631 Cklf (untagged) - Mouse chemokine-like factor (Cklf), transcript variant 1, (10ug) 10 ug
$165.00
MR214563 Cklf (Myc-DDK-tagged) - Mouse chemokine-like factor (Cklf), transcript variant 1 10 ug
$289.00
MR214563L3 Lenti ORF clone of Cklf (Myc-DDK-tagged) - Mouse chemokine-like factor (Cklf), transcript variant 1 10 ug
$450.00
MR214563L4 Lenti ORF clone of Cklf (mGFP-tagged) - Mouse chemokine-like factor (Cklf), transcript variant 1 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.