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Rasgef1a (NM_027526) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214551
Rasgef1a (tGFP-tagged) - Mouse RasGEF domain family member 1A (Rasgef1a), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rasgef1a
Synonyms 6330404M18Rik; AI835194
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214551 representing NM_027526
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCAGACATCTGTGGTCTTCTCCAGCATCCTTGGGCCCAACTGTAACAGACAGGTGCAGCCTGGCA
TGGGGGAGAGAGGAGGTGGGGCCAGCAGCGGCTCCAAGGACCTCATTTTTCAAGATGGACGCCTCACTTC
TGGATCCTTGGAGGCCTTGATGGAGCATCTGGTTCCAACGGTGGACTATTATCCTGATCGGACATACATC
TTCACGTTTCTCCTGAGTTCCCGGGTCTTCATGCCCCCTCATGACCTTCTGGCTCGAGTAGGGCAGATCT
GCCTGGAGCAGAGGCAACAGCTAGAGGCTGGGCCTGAGAAGGCCAAGCTGAAGTCCTTCTCGACCAAGAT
TGTGCAGCTGTTGAAGGAGTGGACGGAGGCCTTCCCTTATGACTTCCAGGACGAGAAGGTCATGGCAGAG
CTGAAGGCCATCGCACACCGCGTCACTCAGTGTGATGAGGAGAATGGCACAGTGAAGAAAGCCATTGCCC
AAATGATACAAAGCTTGCTGCTGTCCCTGGCTGCCCGAAGTCAGCTCCAAGAGCTTCGGGAGAAGCTCCG
GTCACCCGTTGTGGACAAAGGGCCTGTCCTTAAGGCCAAGCCACCAGCTGCCCAGAAAGACATCCTGGGT
GTGTGCAGCGACCCCCTGGTGCTGGCCCAGCAGCTGACTCACATTGAACTGGAACGGGTCAACAGCATCC
GCCCTGAGGACCTGATGCAGATCATCAGCCACATGGACTCTCTGGACAATCACAGGTGCCGTGGAGACAT
GACTAAGACCTTCAGTTTAGAAGCGTATGACAACTGGTTCAACTGCTTGAGCATGCTCGTGGCTACAGAG
GTGTGCCGGGTGGTAAAGAAGAAACACCGGACCCGCATGCTGGAGTTCTTCATTGATGTGGCCCGAGAGT
GTTTCAACATCGGGAACTTCAACTCTATGATGGCCATCATATCTGGCATGAACCTCAGCCCAGTGGCACG
GCTGAAGAAAACGTGGTCCAAAGTCAAGACAGCCAAGTTTGATGTCTTGGAGCATCACATGGACCCCTCC
AGCAACTTCTGCAACTACCGGACAGCCCTGCAGGGGGCGACACAGAGATCCCAGACAGCCAACAGCAGCC
GTGAGAAGATTGTCATCCCCGTGTTCAACCTCTTCGTTAAGGACATCTACTTTCTACACAAAATCCACAC
CAACCACCTACCCAATGGGCACATTAACTTCAAAAAATTTTGGGAAATTTCCAGACAGATCCACGAGTTC
ATGACATGGACACAGGTAGAATGTCCCTTTGAGAAGGACAAGAAGATTCAGAGTTACGTGCTCACGGCGC
CCATCTACAGCGAGGAAGCTCTCTTCATCGCCTCCTTTGAAAGTGAAGGTCCTGAAAATCACATGGAGAA
GGACAGCTGGAAGGCACTCAGGACCACTCTCCTTAACAGAGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214551 representing NM_027526
Red=Cloning site Green=Tags(s)

MPQTSVVFSSILGPNCNRQVQPGMGERGGGASSGSKDLIFQDGRLTSGSLEALMEHLVPTVDYYPDRTYI
FTFLLSSRVFMPPHDLLARVGQICLEQRQQLEAGPEKAKLKSFSTKIVQLLKEWTEAFPYDFQDEKVMAE
LKAIAHRVTQCDEENGTVKKAIAQMIQSLLLSLAARSQLQELREKLRSPVVDKGPVLKAKPPAAQKDILG
VCSDPLVLAQQLTHIELERVNSIRPEDLMQIISHMDSLDNHRCRGDMTKTFSLEAYDNWFNCLSMLVATE
VCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPS
SNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEF
MTWTQVECPFEKDKKIQSYVLTAPIYSEEALFIASFESEGPENHMEKDSWKALRTTLLNRA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027526
ORF Size 1443 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027526.1, NP_081802.1
RefSeq Size 3236 bp
RefSeq ORF 1470 bp
Locus ID 70727
Cytogenetics 6 F1
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Documents
Resources
"NM_027526" in other vectors (4)
SKU Description Size Price
MC216843 Rasgef1a (untagged) - Mouse RasGEF domain family, member 1A (Rasgef1a), (10ug) 10 ug
$503.00
MR214551 Rasgef1a (Myc-DDK-tagged) - Mouse RasGEF domain family, member 1A (Rasgef1a) 10 ug
$289.00 $457.00
MR214551L3 Lenti ORF clone of Rasgef1a (Myc-DDK-tagged) - Mouse RasGEF domain family, member 1A (Rasgef1a) 10 ug
$757.00
MR214551L4 Lenti ORF clone of Rasgef1a (mGFP-tagged) - Mouse RasGEF domain family, member 1A (Rasgef1a) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.