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1700007K13Rik (NM_027040) Mouse Tagged ORF Clone

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SKU
MG214546
1700007K13Rik (tGFP-tagged) - Mouse RIKEN cDNA 1700007K13 gene (1700007K13Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 1700007K13Rik
Synonyms Rbest47
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214546 representing NM_027040
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGAAGAAAAACCTCAACAGTCCGCGGAGGAGCCGGAGCCGGGGGAGCCCAAGGCTAAGCCCGCCC
CAGAGGAGCCGGAGCCGGGGGAGCCCAAGGCTAAGCCTGCCCCAGAGGAGCCGGAGCCGGGGGAGCCCAA
GGCTAAGCCTGCCCCAGAGAAGACCAGCGACTACTACCGCATTAGCGAGAAGCTCCCAGTCAGGTTCAAC
AACCCGGGGTGGTTTCATGGCTACGGGACCAAGGAAGCTGTTTCCATGTACAGGACCAGTAACCAAACCT
ACGGAAGCAGAGCCCCCACGGTGCATGAGATGCCAAAAGTATTTTATCCATCTTCAAATAAGTTTTCCAG
ACAACACGCAGCTTTTGGAATGTTCCAGAGTCATAATATTAATGTCACCCTGGAGAAGAGCTTAGTGACC
GGGCCTGACAATCACATCACCCACTATGACCGTTTGAACTTTCACCCCAGTTACAATGTCAACAGGCCGT
CCATCTGTGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214546 representing NM_027040
Red=Cloning site Green=Tags(s)

MSEEKPQQSAEEPEPGEPKAKPAPEEPEPGEPKAKPAPEEPEPGEPKAKPAPEKTSDYYRISEKLPVRFN
NPGWFHGYGTKEAVSMYRTSNQTYGSRAPTVHEMPKVFYPSSNKFSRQHAAFGMFQSHNINVTLEKSLVT
GPDNHITHYDRLNFHPSYNVNRPSICD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027040
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027040.1, NP_081316.1
RefSeq Size 833 bp
RefSeq ORF 504 bp
Locus ID 69327
UniProt ID Q5BN45
Cytogenetics 2 A3
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Documents
Resources
"NM_027040" in other vectors (4)
SKU Description Size Price
MC210899 1700007K13Rik (untagged) - Mouse RIKEN cDNA 1700007K13 gene (1700007K13Rik), (10ug) 10 ug
$330.00
MR214546 1700007K13Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700007K13 gene (1700007K13Rik) 10 ug
$289.00 $300.00
MR214546L3 Lenti ORF clone of 1700007K13Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700007K13 gene (1700007K13Rik) 10 ug
$600.00
MR214546L4 Lenti ORF clone of 1700007K13Rik (mGFP-tagged) - Mouse RIKEN cDNA 1700007K13 gene (1700007K13Rik) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.