Gm14525 (NM_001162364) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG214518
Gm14525 (tGFP-tagged) - Mouse predicted gene 14525 (Gm14525), (10ug)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm14525
Synonyms OTTMUSG00000017011
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214518 representing NM_001162364
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTATTAAGAAACTGTGGGTGATACCAAAGGATGGTTACTTATTACTTCTTGACTATGATTCAGATG
AAGAAGAAGAGCAGGCTCATTCTGAAGTAAAGAGGCCAGCATTTGGCAAACATGAGAATATGCCGCCTCA
CGTAGAAGCAGATGAAGATATAAGAGATGAACAAGACAGTATGTTGGATAAATCTGGAGAAAACGTAAGT
TTCTCAGAGGAATGGCAGCGTTTTGCACGTTCTGTAGAGACACCAATGGAAAACTGGAATTTGTTAAGTG
GTGAACAGCAAGTTAGGAATGCTTCAGAATTGGACCTTATGGAAGTACAGAATCCAGTAACTCATGATGA
TGGGAATGCAAATCCTGAAGAAGTAGTTGGAGATACACGAAAGAAGATCAACAACAAATTGTGTGAGCAG
AAGTTTGATATGGATATACAGAAATTCAATGAAGAACAAGAAAAATCAGTGAACAATTATCAAAAAGAAC
AACAAGCATTAAAACTGTCCGAATGTAGTCAGAGTCCGACCATGGAAGCAATTGAAGACATGCATGAGAA
GTCCATGGAGGGTTTGATGAACATGGAGACCAACAACTACGATATGCTTTTTGATGTAGATGGTGAAGAG
ACATTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214518 representing NM_001162364
Red=Cloning site Green=Tags(s)

MSIKKLWVIPKDGYLLLLDYDSDEEEEQAHSEVKRPAFGKHENMPPHVEADEDIRDEQDSMLDKSGENVS
FSEEWQRFARSVETPMENWNLLSGEQQVRNASELDLMEVQNPVTHDDGNANPEEVVGDTRKKINNKLCEQ
KFDMDIQKFNEEQEKSVNNYQKEQQALKLSECSQSPTMEAIEDMHEKSMEGLMNMETNNYDMLFDVDGEE
TL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001162364
ORF Size 636 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001162364.1
RefSeq Size 893 bp
RefSeq ORF 639 bp
Locus ID 100039120
Cytogenetics X A2
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Documents
Resources
"NM_001162364" in other vectors (5)
SKU Description Size Price
MC207217 Gm14525 (untagged) - Mouse predicted gene 14525 (Gm14525), (10ug) 10 ug
$330.00
MC215489 Gm14525 (untagged) - Mouse predicted gene 14525 (Gm14525), (10ug) 10 ug
$330.00
MR214518 Gm14525 (Myc-DDK-tagged) - Mouse predicted gene 14525 (Gm14525) 10 ug
$289.00 $300.00
MR214518L3 Lenti ORF clone of Gm14525 (Myc-DDK-tagged) - Mouse predicted gene 14525 (Gm14525) 10 ug
$600.00
MR214518L4 Lenti ORF clone of Gm14525 (mGFP-tagged) - Mouse predicted gene 14525 (Gm14525) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.