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Pcdhb16 (NM_053141) Mouse Tagged ORF Clone

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SKU
MG214463
Pcdhb16 (tGFP-tagged) - Mouse protocadherin beta 16 (Pcdhb16), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $935.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pcdhb16
Synonyms Pcdhb8; PcdhbP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214463 representing NM_053141
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCAGCAGGAAGCTCATTTGCAGACAAAGGCAAGTCCTTTTTTTCTTTCTCCTATTAGGCTTCC
CTCTAACGACTGCAGGGGAACTGAGGCGCTATTCTGTGGTGGAGGAAAAGGAGGGTAGGTCCTTTGTAAC
CAATTTAGTAAAAGACCTGGGTCTTGGGCAGCTGGAACTCTCCCGGAGAGGAGCTAAGGTCATTTCTAAA
GGTAACAAACTACATTTGCAGCTGGATCAGGAGACAGGGGATTTGCTGTTAAATGAGAAAGTGGACAGGG
AAAAACTGTGTGGGCATACAGAGCCATGCTTGCTAAGCTTCCAAGTGTTGCTAGACAACCCCTTGGATAT
TTTTCAAGCTGAGCTAGAAGTCACAGACATAAATGACCACTCACCAGAATTCCTGGACAAAGAGATCATG
CTAAAGATATCAGAAAGCAGTCTTCCTGGAGCTACATTTCCTCTGAAGAATGCTCAGGACATGGATGTAG
GCCAAAACGGTATTGACAAGTATCTGATCAGTTCCAATTCCTATTTTCGAGTGCTCACTCGGAAACGAAG
CGATGGCAAGAAATACCCTGAACTGGTACTAGACAGAGCACTGGATAGAGAGGAGGAAGCTGAGCTCAGG
CTAACCCTCACAGCACAGGATGGTGGCTCTCTGCCTAGGTCTGGAACCACTGAAGTCCACATTGAAGTCC
TGGACATCAATGATAACGCTCCTCAATTTGAGCAGCTTTTTTACAAAGCACAGATCCCTGAGGATAGTCC
AATAGGCTTTCTGATCATCACTGTCTCTGCTACAGACAAGGACATTGGAGTCAATGGACAGATCTCCTAT
TCACTTTTCCAGGTTTCAGATGATATTAGCAAAACATTTTCAATCCACCCCTTGACAGGGGAAGTGCGAT
TGAAAGAACACCTTGATTTTGAAAAGACTCAGTCCTATGAAATCAATATTGAAGCAAGAGACGCTGGGAC
CTTTTCTGGAAAATGCACGATTCTGGCCCAAGTCTTGGATGTGAATGACCATGCTCCAGAGATTATCCTG
TCTGCATTTACCAACTCCATTCTTGAAAACTTACCAGAAACCATGGTGGCAGTTTTTAGCGTTTCTGATC
TAGATTCCGGAGAAAATGGGAAAGTAAGTTGTTCCATTCAGGACGATCTACCCTTCTTCCTAAAGCCTTC
TGGGGAAAACTTTTATACGCTGTTATCACAAAAACCACTGGACAGAGAGAGTGTAGCAGAATACAACATC
ACCATCACCGTTGCAGACATGGGGAGTCCCATCCTGAAAACACAAGTCAACTTAACAGTGCAGGTATCTG
ACATCAACGACAACGCCCCCATCTTCACCCAAACCTCCTATACCATGTTCATCCGTGAGAACAACAGCCC
TGCCCTGCACATAGGCACCATCAGTGCCACAGACTCAGACTCAGGCTCCAATGCTCACATCACTTACTCG
CTGCTGCCGCCCCATGACCACCAGCTGGCCCTCGCCTCGTTCATCTCCATCAACGCAGACAATGGGCAGC
TGTTCGTGCTCAGGGCACTGGACTATGAGGCCCTGCAGGCCTTTGAATTCCATGTGGGTGCCACAGACGG
AGGTTCGCCCGCTCTCAGCAGCCAGGCGCTGGTGCGCATGGTGGTGCTGGACGACAATGACAATGCGCCC
TTCGTGCTCTACCCGATGCAGAACGCCTCTGCACCCTACACTGAGCTGCTGCCCAGGGCGGCAGAGCCCG
GATACCTGGTCACCAAGGTGGTGGCTGTGGACCGCGACTCTGGACAGAATGCCTGGCTGTCGTTCCAGCT
GCTCAAAGCTACGGAGCCCGGGCTGTTCAGTGTGTGGGCACACAATGGAGAGGTACATACCTCCAGGCTG
CTGAGCGAGCGCGATGTGCACAAGCACAGGCTGATACTGCTAGTCAAGGACAATGGCGATCCTCCGCGCT
CTGCCAGTGTCACACTGCATGTGCTGCTGGTGGATGGCTTCTCTCAGCCCTACCTGCCTCTGCCAGAGGT
GGCACGTGATCCTGATCATGAGGACAGTGAGCTCACATTGTACCTGGTCATAGCCTTGGCTTCTGTGTCT
TCTCTCTTCCTGGTGTCTGTGCTGCTGTTTGTGGGGGTGAGGCTGTGCAGGAGAGCCAGGGAGGTCTCTC
TGGGTGGCTGCTCTGTGCCTGAGGGACACTTTCCTGGCCATCTGGTGGATGTCAGTGGCACAGGGACCCT
GTCTCAGAGCTACCAATATGAGGTGTGTCTTACTGGAGACTCTGGGACTGGTGAGTTCAAATATCTGAAA
CCAATTTTACCTAATTTTCAAGACCATTCTTTTAGACCAGAAATGGGTGAAAATCCCAACTCTAGGAATG
ACTGGAGTTTTGGTATTCAGTTAAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214463 representing NM_053141
Red=Cloning site Green=Tags(s)

MEASRKLICRQRQVLFFFLLLGFPLTTAGELRRYSVVEEKEGRSFVTNLVKDLGLGQLELSRRGAKVISK
GNKLHLQLDQETGDLLLNEKVDREKLCGHTEPCLLSFQVLLDNPLDIFQAELEVTDINDHSPEFLDKEIM
LKISESSLPGATFPLKNAQDMDVGQNGIDKYLISSNSYFRVLTRKRSDGKKYPELVLDRALDREEEAELR
LTLTAQDGGSLPRSGTTEVHIEVLDINDNAPQFEQLFYKAQIPEDSPIGFLIITVSATDKDIGVNGQISY
SLFQVSDDISKTFSIHPLTGEVRLKEHLDFEKTQSYEINIEARDAGTFSGKCTILAQVLDVNDHAPEIIL
SAFTNSILENLPETMVAVFSVSDLDSGENGKVSCSIQDDLPFFLKPSGENFYTLLSQKPLDRESVAEYNI
TITVADMGSPILKTQVNLTVQVSDINDNAPIFTQTSYTMFIRENNSPALHIGTISATDSDSGSNAHITYS
LLPPHDHQLALASFISINADNGQLFVLRALDYEALQAFEFHVGATDGGSPALSSQALVRMVVLDDNDNAP
FVLYPMQNASAPYTELLPRAAEPGYLVTKVVAVDRDSGQNAWLSFQLLKATEPGLFSVWAHNGEVHTSRL
LSERDVHKHRLILLVKDNGDPPRSASVTLHVLLVDGFSQPYLPLPEVARDPDHEDSELTLYLVIALASVS
SLFLVSVLLFVGVRLCRRAREVSLGGCSVPEGHFPGHLVDVSGTGTLSQSYQYEVCLTGDSGTGEFKYLK
PILPNFQDHSFRPEMGENPNSRNDWSFGIQLK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053141
ORF Size 2406 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053141.3, NP_444371.3
RefSeq Size 5278 bp
RefSeq ORF 2409 bp
Locus ID 93887
Cytogenetics 18 B3
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Documents
Resources
"NM_053141" in other vectors (4)
SKU Description Size Price
MC221719 Pcdhb16 (untagged) - Mouse protocadherin beta 16 (Pcdhb16), (10ug) 10 ug
$809.00
MR214463 Pcdhb16 (Myc-DDK-tagged) - Mouse protocadherin beta 16 (Pcdhb16) 10 ug
$589.00 $735.00
MR214463L3 Lenti ORF clone of Pcdhb16 (Myc-DDK-tagged) - Mouse protocadherin beta 16 (Pcdhb16) 10 ug
$1,035.00
MR214463L4 Lenti ORF clone of Pcdhb16 (mGFP-tagged) - Mouse protocadherin beta 16 (Pcdhb16) 10 ug
$1,035.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.