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Fam186b (NM_001081254) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214431
Fam186b (tGFP-tagged) - Mouse family with sequence similarity 186 member B (Fam186b), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,063.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Fam186b or NM_001081254"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fam186b
Synonyms EG545136
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214431 representing NM_001081254
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGGACAACCCCCCACACTTGGTGACTCCCACATCAGTGAAGGCTATTATCTCAAAGATTGAGA
CTGCCCAGCTAATTCGGACTCAGGAGGATATTTCCACCCAGCTCTCGGACATCTTGGACAATGTCAACTG
TGCCATCAACCGCTTCCAGGAAGAACTGGGATATGACTTAAAAGAAAGAGCGAAGCCTCACCAACCAGAG
CAGAAAGGCAAGAAGAGGTTCATCTTGCTGGAGAAACTCGCCGCCTTCTCTGATGACGCCAAGGCCAAGG
AGAAGCACCTGTATGAGATCCTCCACTGGCTGGGCGAATGGGGTGACAGTCTGACTTATGAGATAAAGGA
CAGGAGTTCGGAGGAAGACGAAGCCCTAGACGAATGGATCGAAGTGATGGAGAAGGTGTTGCCTCTTTCC
CTCATGGCCACCAAAGGAGGCATCGAGTCTCTCATCTCTCTGTGCTCGACTCTCATCGAAGACCAGAAGA
AAATGACACAAAGGTACAAACATAACTTCTGGCAGGACTGGCATGAGAAGAGCCCACAGAATAAGGAGCA
GGGAATCCCTGTGCCACCGAGCCCGGAGCAGATGCTCCAGGACAAGAACACCGCCCACCTGAACGTCTCG
GAGGTCAGGTCCATGCTACAGGAGCTCCTGGACTCCACGATGTTCAACAAAGGGGAGGTTAAAGCCATCA
GGTACATGTCCGCCGTGGTGGAGAACCTCAACAAGGCCTTGACCCTCCAGCACAAGGAGAACAAGAGTCT
GGAGACAAAGTACAAGTACCTGCAGGTGGAAAAGAACAAAGAGCTCAGCACCCAGAGGCTGCACTTCCAA
AAATCCATCCAAGTCCTGGAGAGCAAAAGGGACGCGCTGCTGAAACAGGTAAAGATTCTAGGGGACAAAT
ACCATCACCTCCTCATGTTAAAGCACACCCTGGAGTTCCAGCTGAATATAGCCCAGCCTGCTAGTGGTCC
AGAAGAACCCACCAAGCATTTCATTCATTACCTGGAACCCCCTGAAAAAGAGCCCCTTCCAGGGCAGGGT
ACAGCCCTGGAGAAGGAGAAGCAGGAGCCCAAGGAGAAAGGACAGGCAGTTTCAGCTCTCTTTTCAAGTC
CCCTGACCAAGGCCTGGCACAGTGGCACTACACCTGCAGCCCCAAAGCCAATCTTCGGCACGGACACGGA
TTCGAGGGTGAGGGATGTGTTCGTTGACCGCACAGAGGCCCTGGAGCCTGTGCTGCTGCACTCAGAGGTC
CCCCAGATTCCCACACAATGGGAGAAACTAGGGGACATGAAAGACAAAGACCAGGGGGATCACATCCAAG
AGGAGGGAGCCCAAAGCAAGTCCCTGTCCCCAGGGAGCATCAGGAGGAGGCTTCTGGAGAGCCACATGGG
GCACTGGGAGGAAGAGCTCAACTGGGAGACCAGAAGGCAGCAATGGCTGCAGGAGGAAGAGATGTGGCTT
CAGCGGCAGAAGAAGTGGGCCCTGCTGGAGCAGGAGCACCAGGAAAAGGTGAGGCAGTGGGAGGCAGAGG
CGGCTGCCAGGCAGCAGTGGCAGAGGCTCACCCAGCCAGAGGAGGAGAAGCCCAGCCCCAGGAAGGAGCA
GAAGGCAAGCTCGGAGATGATCTTCACCACCACCAGTCGGTGGAAGAGCCTGAAGAAGTCGGAGTCCACG
CCGACGCCCCACTGCAGGGCCCAGTCCGCGCATCAGAGCAGGTCGTACATACTCGCATCCCCACACACCC
AGCAGCCTGGGCAGGGGACCCAGAGGACCCTGGGTTCAGCTGAGTTTCTGCAAAGGCCACCGACCCGCCA
GGTTTCTGCGAAGCCCAAGAAATCCACCTCCTTGCCAGTCACTGGGACGTCCATCCTCCGAAGGGTGTCC
CAGGTCTCTCTGCAGCAAGCCCCGGGGACACCTAAGGACAAGGTCTACCACATAGACATGGAGGCCCAGC
TGAAGAACCTGCAGGTCCTGGGCGGTTCGGAGTCAGAGGTGGCACTGCCACAGTACCTGCGCAGCAAAGC
CCTGGAGGTCATTGCCATCGCCATGGACCTGAGCATTCTCCGGCTGCAGTACCTATGCAAGAAGTATATC
CAATACAGGCGCTTCCAGAGCCTCCGACTAGAAATGATCAAACACATAGGAGCCATTCGACAAACAAGAG
TTACCAGTAAGGCCCAGAACCTCTATCTGTTCCTGGAGAACATTGACCACCAGCAGAGCCTCAGGCTGCA
GGCCTGGGCAGACAAGCAGAGAGACCTGGAGGAAAGGCACCAGGAATGCCTGCACACCATGGTGGCCATG
TTCCCTAAGCTCCAGCAAGAGTGGAACGTCCACCTGAGCGTCCCCGTGCTCACCCATGTGAAGCCAGTGA
AGAGCAAGTCATCTTCAGTCTTGCTTCAGAGAGTCCGCTCCAGCCACTCCACCACCAAGCACCGTCCTCC
ACCTAAGCCCCGGGAGTCTGTGCCCCTGCCGATAACTGGCCAACAAGGGAGCCAGATGGAAGCCATATGG
AAGGCTGATGTGGCCTCTTCGAGTCACCCCATAGAAAAGAAGACCCCTACAAACATGTGCTGGGAACAAC
TAGGGGGCTACCCAGACATCCCCAGGTTACTGGTGGTGGACGAGCATCCCTCCTGCCCCAGAAGCTCGGT
GTCCTTCAAGGCTCGCTCTTTCAGTGCTTCCTCGGTCCAAAGGAAGGAAGGCCAGGAACCCTCAGAGGAG
CTAGCTGGGCCTGTCTGCAAAATGTCCAGCCAGTCACTACCCGGATCGATCTGTACAAAGCAGGAAGGAT
CGGGCAGCTCCAGTACCCACTCCATCCCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214431 representing NM_001081254
Red=Cloning site Green=Tags(s)

MEKDNPPHLVTPTSVKAIISKIETAQLIRTQEDISTQLSDILDNVNCAINRFQEELGYDLKERAKPHQPE
QKGKKRFILLEKLAAFSDDAKAKEKHLYEILHWLGEWGDSLTYEIKDRSSEEDEALDEWIEVMEKVLPLS
LMATKGGIESLISLCSTLIEDQKKMTQRYKHNFWQDWHEKSPQNKEQGIPVPPSPEQMLQDKNTAHLNVS
EVRSMLQELLDSTMFNKGEVKAIRYMSAVVENLNKALTLQHKENKSLETKYKYLQVEKNKELSTQRLHFQ
KSIQVLESKRDALLKQVKILGDKYHHLLMLKHTLEFQLNIAQPASGPEEPTKHFIHYLEPPEKEPLPGQG
TALEKEKQEPKEKGQAVSALFSSPLTKAWHSGTTPAAPKPIFGTDTDSRVRDVFVDRTEALEPVLLHSEV
PQIPTQWEKLGDMKDKDQGDHIQEEGAQSKSLSPGSIRRRLLESHMGHWEEELNWETRRQQWLQEEEMWL
QRQKKWALLEQEHQEKVRQWEAEAAARQQWQRLTQPEEEKPSPRKEQKASSEMIFTTTSRWKSLKKSEST
PTPHCRAQSAHQSRSYILASPHTQQPGQGTQRTLGSAEFLQRPPTRQVSAKPKKSTSLPVTGTSILRRVS
QVSLQQAPGTPKDKVYHIDMEAQLKNLQVLGGSESEVALPQYLRSKALEVIAIAMDLSILRLQYLCKKYI
QYRRFQSLRLEMIKHIGAIRQTRVTSKAQNLYLFLENIDHQQSLRLQAWADKQRDLEERHQECLHTMVAM
FPKLQQEWNVHLSVPVLTHVKPVKSKSSSVLLQRVRSSHSTTKHRPPPKPRESVPLPITGQQGSQMEAIW
KADVASSSHPIEKKTPTNMCWEQLGGYPDIPRLLVVDEHPSCPRSSVSFKARSFSASSVQRKEGQEPSEE
LAGPVCKMSSQSLPGSICTKQEGSGSSSTHSIP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081254
ORF Size 2829 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081254.1, NP_001074723.1
RefSeq Size 3187 bp
RefSeq ORF 2832 bp
Locus ID 545136
Cytogenetics 15 F1
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Documents
Resources
"NM_001081254" in other vectors (4)
SKU Description Size Price
MC222769 Fam186b (untagged) - Mouse family with sequence similarity 186, member B (Fam186b), (10ug) 10 ug
$951.00
MR214431 Fam186b (Myc-DDK-tagged) - Mouse family with sequence similarity 186, member B (Fam186b) 10 ug
$589.00 $863.00
MR214431L3 Lenti ORF clone of Fam186b (Myc-DDK-tagged) - Mouse family with sequence similarity 186, member B (Fam186b) 10 ug
$1,163.00
MR214431L4 Lenti ORF clone of Fam186b (mGFP-tagged) - Mouse family with sequence similarity 186, member B (Fam186b) 10 ug
$1,163.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.