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Pcdhb5 (NM_053130) Mouse Tagged ORF Clone

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SKU
MG214395
Pcdhb5 (tGFP-tagged) - Mouse protocadherin beta 5 (Pcdhb5), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $925.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pcdhb5
Synonyms Pcdhb4A; PcdhbE
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214395 representing NM_053130
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGCTGGAGAGAAATCATCGAAACAGGCAAGTGATTCCTTTCCTTTTTATGCTGCTTTGGGATC
AGGTCCTCATGGTGCCTACTCGGTACTCTGTCTTGGAGGAATCAGAAAGTGGCTCCTTTGTAGCCCATCT
GGCCAAGGATCTGGGTCTGGGAGCTGGGGAGCTGGCTGCCAGGTCTGCACGGGTGGTGTCTGACCATGAC
AAACAGCAATTCATTCTAGATTCTGAGACTGGAGATTTGCTTCTGAGGGAGAAACTAGACAGGGAAGAAC
TGTGTGGCTCTGTGGATCCCTGTGTGCTGCATTTCCAAGTGTTCCTAGAAAAGCCAGTGCAGTTTTTTCA
AGGAGAATTATTGATCCAGGATATAAATGACAACTCGCCAGAATTCCCAGATAAAGAATTGCTCTTGAAA
ATACTGGAAAACAGCCAGCCAGGCACACGGTTTTCATTAAAATTAGCCCAGGATTTGGATGTGGGCAGCA
ATGGGCTTCAACAATACACAGTCAACCCCAACTCTCATTTTCATGTCCTCACTCGAAATAATAGCGAGGG
CAAGAAATACCCAGAGTTGGTACAGGACAGAGCCCTGGACAGAGAGGAGCAGGCAGAGCTGAGCTTAATC
CTCACAGCTCTGGATGGCGGGTCTCCACCGAGGTCAGGAACAGCCCTGGTTCGAATCCTGATCATGGACA
TCAATGACAATGCTCCTGAGTTTGTGAACAACCCCTATGAGGTGCAGGTCCTAGAGAGCAGTCCCCCAGA
CTCCCCAGTCCTGACTGTCTTCGCACAGGATGCAGATGCTGGCAACTTTGGGAGAGTTTCCTATGGCTTG
TTCCAAGCATCAGATGAAATCCAGAAAACTTTCTCAATAAACGAATTCACTGGAGAAATACGACTGAAAA
CGAAATTGGATTTTGAAAAAACTAAATCTTACCATGTAGAAATTGAGGCCACAGATGGAGGAGGCCTTTC
TGGGAAGGGCTCTGTGGTGATAGAGGTGTTGGATGTGAACGACAATGCCCCAGAGCTGACCATATCTTCA
CTGACCAGCTCAGTCCCAGAAAATTCTCCAGAAACTGTGGTCGCTATCATCCGAATACGGGATAGAGATT
CTGGAGAGAATGGGAAGATGATTTGTTCAATTTCAGATCATGTGCCATTCATTTTAAAACCCTCTTACAA
GAACTTTTACACCCTGGTGACAGAGAGCCCTCTGGATAGAGAGAGCAGAGCTGAGTACAACATCACCATC
ACAGTCTCCGACCTGGGCACACCCAGGCTCACAACCCAGCACACCATAACAGTGAAGGTGTCCGACATTA
ACGACAACGCCCCTGCCTTCAGCCAAACCTCCTACACCATGTTTGTCCGCGAGAACAACAGCCCTGCCCT
GCACATTGGCACCATCAGTGCCACAGACTCAGACTCAGGCTCCAATGCTCACATCACCTACTCGCTGCTG
CCACCCCACGACCAACAGCTGGCCCTCCACTCGCTCATCTCCATCAATGCTGACAATGGGCAGCTATTTG
CGCTCAGTGCGCTGGACTATGAGGCCCTGCAGGGCTTCGAATTCTACGTGGGTGCCACAGACCGAGGCTC
TCCTGAGCTCAGCAGCCAGGCTCTGGTGCGCGTGGTGGTGCTAGACGACAATGACAATGCGCCCTTCGTG
CTCTACCCACTGCAGAATGCTTCTGCGCCCTGCACTGAGGTGCTGCCCAGGGCTGCGGAGCCTGGATACC
TGGTCACCAAGGTGGTGGCTGTGGACCGCGACTCTGGCCAGAATGCCTGGTTGTCATTCCAGCTGCTCAA
GGCCACGGAGCCCGGACTGTTCAGCGTGTGGGCTCACAATGGCGAGGTGCGCACCACCAGGCTGCTGAGT
GAGCGAGATGCACCCAAGCACAGGCTGCTGCTGCTGGTCAAGGACAATGGAGAGCCTCAGCGCTCTGCCA
GCGTCACGCTGCACGTGTTGCTGGTGGATGGCTTCTCTCAACCTTACCTGCCCCTGCCTGAAGTGGCGCG
CGACCCCGCACAGGATGATCCGGATGTGCTCACGCTCTACCTTGTCATTGCCTTGGCCTCTGTGTCTTCT
CTCTTTCTTTTGTCTGTGCTGATGTTCGTGGGAGTGAGGCTGTGCAGGAGGGCCAGGGAACCCTCTCTGG
GTGGCTGCTCTGTGCCTGAGGGCCACTTTCCTGGCCACCTGGTGGATGTGAGCCATGCAGGGACCCTGTC
CCAGAGCTACCAGTATGACATGTGTCTAACAGGAGAATCTGGGACTGGTGAGTTCAAATTCCTCAAGCCC
ATAGTCCCCAACCTAATGGTTCAGGATACGGGAAATCTTCACTGCAGGGATAGCTTTGTATTCAGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214395 representing NM_053130
Red=Cloning site Green=Tags(s)

MEKLERNHRNRQVIPFLFMLLWDQVLMVPTRYSVLEESESGSFVAHLAKDLGLGAGELAARSARVVSDHD
KQQFILDSETGDLLLREKLDREELCGSVDPCVLHFQVFLEKPVQFFQGELLIQDINDNSPEFPDKELLLK
ILENSQPGTRFSLKLAQDLDVGSNGLQQYTVNPNSHFHVLTRNNSEGKKYPELVQDRALDREEQAELSLI
LTALDGGSPPRSGTALVRILIMDINDNAPEFVNNPYEVQVLESSPPDSPVLTVFAQDADAGNFGRVSYGL
FQASDEIQKTFSINEFTGEIRLKTKLDFEKTKSYHVEIEATDGGGLSGKGSVVIEVLDVNDNAPELTISS
LTSSVPENSPETVVAIIRIRDRDSGENGKMICSISDHVPFILKPSYKNFYTLVTESPLDRESRAEYNITI
TVSDLGTPRLTTQHTITVKVSDINDNAPAFSQTSYTMFVRENNSPALHIGTISATDSDSGSNAHITYSLL
PPHDQQLALHSLISINADNGQLFALSALDYEALQGFEFYVGATDRGSPELSSQALVRVVVLDDNDNAPFV
LYPLQNASAPCTEVLPRAAEPGYLVTKVVAVDRDSGQNAWLSFQLLKATEPGLFSVWAHNGEVRTTRLLS
ERDAPKHRLLLLVKDNGEPQRSASVTLHVLLVDGFSQPYLPLPEVARDPAQDDPDVLTLYLVIALASVSS
LFLLSVLMFVGVRLCRRAREPSLGGCSVPEGHFPGHLVDVSHAGTLSQSYQYDMCLTGESGTGEFKFLKP
IVPNLMVQDTGNLHCRDSFVFS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053130
ORF Size 2376 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053130.3, NP_444360.1
RefSeq Size 3533 bp
RefSeq ORF 2379 bp
Locus ID 93876
Cytogenetics 18 B3
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Documents
Resources
"NM_053130" in other vectors (4)
SKU Description Size Price
MC221647 Pcdhb5 (untagged) - Mouse protocadherin beta 5 (Pcdhb5), (10ug) 10 ug
$799.00
MR214395 Pcdhb5 (Myc-DDK-tagged) - Mouse protocadherin beta 5 (Pcdhb5) 10 ug
$589.00 $725.00
MR214395L3 Lenti ORF clone of Pcdhb5 (Myc-DDK-tagged) - Mouse protocadherin beta 5 (Pcdhb5) 10 ug
$1,025.00
MR214395L4 Lenti ORF clone of Pcdhb5 (mGFP-tagged) - Mouse protocadherin beta 5 (Pcdhb5) 10 ug
$1,025.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.