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4921539E11Rik (NM_027612) Mouse Tagged ORF Clone

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SKU
MG214383
4921539E11Rik (tGFP-tagged) - Mouse RIKEN cDNA 4921539E11 gene (4921539E11Rik) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "4921539E11Rik or NM_027612"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 4921539E11Rik
Synonyms RP23-47A6.2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214383 representing NM_027612
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGAAAAAATCCTGGAAAAGTTTGATGCCCTGGAGGAGAAAGAAAGGATACTTGAAGCCATCAGGG
AAAATAATACTGACCGTCACCACCGACAAAGAAAAAAGCCTTTGATTACACCCCTGCTATTTGACCTTCA
TGTGCAATTTGGAGACACAATTACCCCATCTGCATCTAAGATCATAAAAAATAAACCACATGATCTTAAG
AACCCAAAAAGGTCTGTGTCTTTCAAATATAAGCCTAACAACTCCAGAAGTGATCTTGAAGAGTCAGATC
TAAGACCACCTATTCTTGGAACAATGATAAACTATGAAGAAAGTAAATTAATGGATCACAAAGAAGAAAA
TCTAAAATCAAGACCTATATCTCTTCGTTATCTTAAGGATAAAGATGAGACAGAATACGCCAATCCCCTG
CCTTTTCCACAGCTTTGGTCTAAACACCTGTGTAAAAAATCAGCGGAGTCTTCAGTCCCGACTCCCAAAC
TCACCAATGAATCCAATGCTAGTAAGAAGGAAAACGTCTCTCCTCCATTTACAGATCAACATGAAAGCAG
AACTAAGAAGTCCATGCATTCTACAGACCACTCAGCAGATTCTAGTACAAGCAGGGGGAAATGCCCTCCA
AAGGGTATTACAAAAGAAAGTGAGTTAACCAGAAATGATGAAGCCAGAAAGCCCCATCCGGTCAAACAGA
GCATCATGCTTCCCCTGGACTGTGAGGACCTACTGAAAAATCCAAAAATAAAGACAATTGACCTTCGTCC
AGCAGTGACAGTACATACTTCCATGAAACAATCCCACGCAAATCCAATAATTTTCTATGACACTGAATAT
GTACAAATGCTGTTCCTGACCAAAAGGTTTACCCCCTACGCTATGAAATGTACAGAAAGGAATATTGTAT
TAGAAAAAAATTATGAAGTACTCAAAGCTTTGTTTAGTGATGAACCAAGTGCTGTTTCTGAACCTATCCA
GCAAAAACATTTGCAAGTCTTCTCTGCTGAATATGCCCAAAAGAGTATAAATGAGAAACGAAAGAAGAAA
CATGACCATCTGGTTTCAAAGAAAATATCTCCGAACACTCATTATAATTTATCCCAAACGTTTTCCAGCT
TAAGCAAAAAATTTGTTGGTTACTTTGATAAAGATGTTACTCAAGGAAAGAGTTATAAGGCAAACAGATT
TGAAAGGTTTTCCAAAACAAAGCCACCACCCACACGCAAACTTACTACCTTACCTATCAAGTATGACTCC
AAGCCTCTGAAAAATATATTTGAAATACATAAACTAAACAACATGACCCCATTGGATAACCTGCTAGGCT
TGAGAGCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214383 representing NM_027612
Red=Cloning site Green=Tags(s)

MTEKILEKFDALEEKERILEAIRENNTDRHHRQRKKPLITPLLFDLHVQFGDTITPSASKIIKNKPHDLK
NPKRSVSFKYKPNNSRSDLEESDLRPPILGTMINYEESKLMDHKEENLKSRPISLRYLKDKDETEYANPL
PFPQLWSKHLCKKSAESSVPTPKLTNESNASKKENVSPPFTDQHESRTKKSMHSTDHSADSSTSRGKCPP
KGITKESELTRNDEARKPHPVKQSIMLPLDCEDLLKNPKIKTIDLRPAVTVHTSMKQSHANPIIFYDTEY
VQMLFLTKRFTPYAMKCTERNIVLEKNYEVLKALFSDEPSAVSEPIQQKHLQVFSAEYAQKSINEKRKKK
HDHLVSKKISPNTHYNLSQTFSSLSKKFVGYFDKDVTQGKSYKANRFERFSKTKPPPTRKLTTLPIKYDS
KPLKNIFEIHKLNNMTPLDNLLGLRA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027612
ORF Size 1338 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027612.1, NP_081888.1
RefSeq Size 2448 bp
RefSeq ORF 1341 bp
Locus ID 70941
UniProt ID Q9D5Q8
Cytogenetics 4 C6
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Documents
Resources
"NM_027612" in other vectors (4)
SKU Description Size Price
MC216006 4921539E11Rik (untagged) - Mouse RIKEN cDNA 4921539E11 gene (4921539E11Rik), transcript variant 1, (10ug) 10 ug
$503.00
MR214383 4921539E11Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4921539E11 gene (4921539E11Rik), transcript variant 1 10 ug
$289.00 $457.00
MR214383L3 Lenti ORF clone of 4921539E11Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4921539E11 gene (4921539E11Rik), transcript variant 1 10 ug
$757.00
MR214383L4 Lenti ORF clone of 4921539E11Rik (mGFP-tagged) - Mouse RIKEN cDNA 4921539E11 gene (4921539E11Rik), transcript variant 1 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.