Wdr86 (NM_001081441) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG214330
Wdr86 (tGFP-tagged) - Mouse WD repeat domain 86 (Wdr86), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wdr86
Synonyms 2810046M22Rik; BC059069
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214330 representing NM_001081441
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGCAGCGGGTCGGCTCTGAGGGTCTGTGCGGACCACCGCGGGGGCATCAACTGGCTGAGCCTGA
GCCCCGACGGGCAGCGCCTGCTGACGGGCAGCGAGGACGGCACAGCCCGCCTCTGGAGCACCGTGGACGG
CCAGTGCTGCGCGCTCCTTCAAGGACATGAGAGCTATGTGACCTTCTGTCACCTGGAGGATGAAGCTGCC
TTCACCTGTAGTGCTGACTGCACCATCCGGAGGTGGGATGTGAGGACGGGGCAGTGTCTGCAGGTGTACA
GAGGACACACATCCATTGTGAACAGGATCCTAGTTGCCAATGACCAGCTCTTCAGCAGTTCCTATGATCG
GACAGCTCGTGTGTGGACTGTGGACAAAGGGCAAGTGTCCCAAGAGTTCCGGGGCCACCGTAACTGTGTA
CTGACACTAGCCTACTCTGCTCCCGAGGACCTGCCCCAAGATCCCTGCATGGAGGCAGCCATGGGTGGTG
GGCTCCTAGTGACTGGCAGCTCAGATGACACAGCCAAAGTGTGGCAGGTAGCCAGTGGCTGCTGCCACCA
GACTCTTCGGGGCCACACAGGTGCAGTGCTGTGCCTGGTGCTGCATGTGTCCAGCCACACAGCCTTCACA
GGCAGCACTGATGCCACCGTTCGCGCTTGGGACATCCTGAGTGGGGAGCAGCTGCGAGTTTTCCGGGAGC
ACCAGGGCTCTGTCATCTGTTTGGAGCTCACAGATCGGCTCCTGTACTCGGGCAGTGCGGACAGAACTGT
CAAGTGCTGGCTAGCTGATACTGGGGAGAGGGTGCTCACCTTTCCAGCACATAGACACAGCGTGAGTGCC
CTCAAGTACCACGCAGGCACCTTGTTCACAGGCAGTGGGGATGCCTGCGCCCGGGCCTTTGATGCCCAGT
CAGGAGTGCTGCAGAGGGTGTTCCGAGGCCACACCTTTGTCATCAACTGTCTCCAGGTGCACGGCCAGGT
GCTCTACACAGCTTCTCATGATGGTGCTCTGCGCCTCTGGGACGTACGTGGTCTCCAGTCGGTGCCACCT
TTGCCACGCAGGCCAGCAGCCAAGCGTAGCCTCTCACGCCTCTTTAGCAACAAGGTGGCCTGTGCAGCCC
CAGTGCCCCTACAGCCAGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214330 representing NM_001081441
Red=Cloning site Green=Tags(s)

MGSSGSALRVCADHRGGINWLSLSPDGQRLLTGSEDGTARLWSTVDGQCCALLQGHESYVTFCHLEDEAA
FTCSADCTIRRWDVRTGQCLQVYRGHTSIVNRILVANDQLFSSSYDRTARVWTVDKGQVSQEFRGHRNCV
LTLAYSAPEDLPQDPCMEAAMGGGLLVTGSSDDTAKVWQVASGCCHQTLRGHTGAVLCLVLHVSSHTAFT
GSTDATVRAWDILSGEQLRVFREHQGSVICLELTDRLLYSGSADRTVKCWLADTGERVLTFPAHRHSVSA
LKYHAGTLFTGSGDACARAFDAQSGVLQRVFRGHTFVINCLQVHGQVLYTASHDGALRLWDVRGLQSVPP
LPRRPAAKRSLSRLFSNKVACAAPVPLQPA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081441
ORF Size 1140 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081441.1, NP_001074910.1
RefSeq Size 1805 bp
RefSeq ORF 1143 bp
Locus ID 269633
Cytogenetics 5 A3
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Documents
Resources
"NM_001081441" in other vectors (4)
SKU Description Size Price
MC214289 Wdr86 (untagged) - Mouse WD repeat domain 86 (Wdr86), (10ug) 10 ug
$503.00
MR214330 Wdr86 (Myc-DDK-tagged) - Mouse WD repeat domain 86 (Wdr86) 10 ug
$289.00 $457.00
MR214330L3 Lenti ORF clone of Wdr86 (Myc-DDK-tagged) - Mouse WD repeat domain 86 (Wdr86) 10 ug
$757.00
MR214330L4 Lenti ORF clone of Wdr86 (mGFP-tagged) - Mouse WD repeat domain 86 (Wdr86) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.