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Medag (NM_027519) Mouse Tagged ORF Clone

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SKU
MG214305
Medag (tGFP-tagged) - Mouse RIKEN cDNA 6330406I15 gene (6330406I15Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Medag or NM_027519"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Medag
Synonyms 6330406I15Rik; Awms3; mAWMS3; MEDA-4; Meda4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214305 representing NM_027519
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTGCAGCGTGCGAGCCTGTGGCCAGACCCAGCCTGACCTCCATTTCATCCGGGGAGCTGCGCA
GTCTGTGGACGTGCGACTGCGAGCTGGCCCTGCTGCCCCTGTCGCAGCTGCTCCGCCTACAGCCTGGTGC
CTTCCAACTGCGCGGTGAGCAGCTCCTGGTGCCCGGGCCCGGGGAACCCGCGGCCGAACGTGGGGGCTTC
AACGTCTTCGGGGACGGCCTTGTGCGCCTAGACGGGCAGCTCTATCGCCTCAGCAGCTACATCAAGAGAT
ATGTGGAACTGACCAACTACTGTGATTATAAAGACTACAGGGAAACGATACTGAGCAAACCTATGGTATT
CTTTATTAACGTGCAGACCAAGAAGGACATTTCAAAAGAAAGGACCTATGCGTTTCTTGTGAACACAAGG
CACCCCAAGATAAGAAGACAGATAGAGCAAGGGATGGACATGGTCATTTCCTCAGTGATTGGAGAAAGCT
ATAGACTTCAGTTTGATTTCCAGGAAGTAGTGAAGAATTTCTTCCCTCCAGGAACGATTGTGCTGAATGG
AGAGAATTTGAGCTTCACCTACGAATTCAAGGCAGATGCCCTGTTTGATTTCTTCTACTGGTTTGGGCTC
AGCAATTCCACCGTAAAAGTGCACGGAAAGGTTCTGAACTTGACAAGTACGAACCCAGAAAAGAAAGAAA
CAATCAAGTTATTTCTGGAAAAAATGAGTGAACCTCTAATACGAAGGAGTAGTTTCTCTGATCGGAAATT
TAGCGTCACATCTAGAGGTTCAATAGATGACGTTTTCAACTGCAATCTGTCACCCAGATCATCGGTTACA
GAACCACTTTTGGCAGAATTCTCATTTCCAAGTCTTCTGGAATGTGAAGAGACATCCAGCCAACTTATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214305 representing NM_027519
Red=Cloning site Green=Tags(s)

MATAACEPVARPSLTSISSGELRSLWTCDCELALLPLSQLLRLQPGAFQLRGEQLLVPGPGEPAAERGGF
NVFGDGLVRLDGQLYRLSSYIKRYVELTNYCDYKDYRETILSKPMVFFINVQTKKDISKERTYAFLVNTR
HPKIRRQIEQGMDMVISSVIGESYRLQFDFQEVVKNFFPPGTIVLNGENLSFTYEFKADALFDFFYWFGL
SNSTVKVHGKVLNLTSTNPEKKETIKLFLEKMSEPLIRRSSFSDRKFSVTSRGSIDDVFNCNLSPRSSVT
EPLLAEFSFPSLLECEETSSQLI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027519
ORF Size 909 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027519.3, NP_081795.1
RefSeq Size 2876 bp
RefSeq ORF 912 bp
Locus ID 70717
UniProt ID Q14BA6
Cytogenetics 5 G3
Summary Involved in processes that promote adipocyte differentiation, lipid accumulation, and glucose uptake in mature adipocytes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027519" in other vectors (4)
SKU Description Size Price
MC211096 Medag (untagged) - Mouse RIKEN cDNA 6330406I15 gene (6330406I15Rik), (10ug) 10 ug
$330.00
MR214305 Medag (Myc-DDK-tagged) - Mouse RIKEN cDNA 6330406I15 gene (6330406I15Rik) 10 ug
$289.00 $300.00
MR214305L3 Lenti ORF clone of Medag (Myc-DDK-tagged) - Mouse RIKEN cDNA 6330406I15 gene (6330406I15Rik) 10 ug
$600.00
MR214305L4 Lenti ORF clone of Medag (mGFP-tagged) - Mouse RIKEN cDNA 6330406I15 gene (6330406I15Rik) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.