Bud23 (NM_025375) Mouse Tagged ORF Clone

CAT#: MG212791

Wbscr22 (tGFP-tagged) - Mouse Williams Beuren syndrome chromosome region 22 (Wbscr22), (10ug)

Product Images

Circular map for MG212791

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bud23
• Synonyms: 1110003N24Rik; Wbscr22
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212791 representing NM_025375
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTCGTAGCCGGAGACCCGAACACAGCGGACCGCCGGAGCTGTTTTATGACCAGAATGAAGCCC
  GGAAATACGTTCGCAACTCACGGATGATTGACATCCAGACCAAGATGACTGAGCGAGCGCTGGAGCTCCT
  CTGTTTACCAGAGGGTCAGCCTTCTTACCTGTTAGACATTGGCTGCGGTTCTGGGCTGAGTGGAGATTAT
  ATCTCAGAAGAGGGACACTACTGGGTGGGCATTGACATCAGCCCTGCCATGTTGGATGCCGCCTTGGACC
  GAGATACAGAGGGGGACCTGCTGCTAGGGGACATGGGCCAGGGCGTCCCTTTCAGACCGGGCTCTTTTGA
  TGGCTGCATCAGCATCTCTGCTGTGCAGTGGCTCTGCAACGCCAACAAGAAGTCGGACGTCCCTGCCAGG
  CGCCTGTACTGCTTCTTTTCTTCCTTGTACTCTGCCCTTGTCCGTGGGGCCCGAGCTGTCCTGCAGCTGT
  ACCCTGAGAACTCGGAGCAGCTGGAGCTGATCACAACCCAGGCCACGAGGGCAGGCTTCACTGGCGGCGT
  GGTGGTAGACTTCCCCAACAGTGCCAAAGCAAAGAAGTTCTACCTCTGTCTGTTTTCTGGGCCTTCCACC
  TCCCTGCCAAAGGGGCTGACTGAAAGTCAGGATGCAGACCAGGCCTCCGAGTCCATGTTCACCAGTGAGC
  GGGCCCCACACAAGAAGGCACGGAGGGACCTGGTGAAGAAGAGCCGGGAATGGGTCCTAGAGAAGAAGGA
  GAGGCGCAGGCGCCAGGGCAAGGAGGTCAGACCTGACACCCAGTACACCGGCCGAAAGCGCAAGCCCCGC
  TTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212791 representing NM_025375
  Red=Cloning site Green=Tags(s)
  
  MASRSRRPEHSGPPELFYDQNEARKYVRNSRMIDIQTKMTERALELLCLPEGQPSYLLDIGCGSGLSGDY
  ISEEGHYWVGIDISPAMLDAALDRDTEGDLLLGDMGQGVPFRPGSFDGCISISAVQWLCNANKKSDVPAR
  RLYCFFSSLYSALVRGARAVLQLYPENSEQLELITTQATRAGFTGGVVVDFPNSAKAKKFYLCLFSGPST
  SLPKGLTESQDADQASESMFTSERAPHKKARRDLVKKSREWVLEKKERRRRQGKEVRPDTQYTGRKRKPR
  F
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025375
• ORF Size: 843 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025375.4
• RefSeq Size: 1323 bp
• RefSeq ORF: 846 bp
• Locus ID: 66138
• UniProt ID: Q9CY21
• Cytogenetics: 5 G2
• Gene Summary: S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(7) position of a guanine in 18S rRNA. Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity. Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity. Locus-specific steroid receptor coactivator. Potentiates transactivation by glucocorticoid (NR3C1), mineralocorticoid (NR3C2), androgen (AR) and progesterone (PGR) receptors. Required for the maintenance of open chromatin at the TSC22D3/GILZ locus to facilitate NR3C1 loading on the response elements. Required for maintenance of dimethylation on histone H3 'Lys-79' (H3K79me2), although direct histone methyltransferase activity is not observed in vitro.[UniProtKB/Swiss-Prot Function]