Cactin (NM_027381) Mouse Tagged ORF Clone

CAT#: MG212579

Cactin (tGFP-tagged) - Mouse RIKEN cDNA 2510012J08 gene (2510012J08Rik), (10ug)

Product Images

Circular map for MG212579

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cactin
• Synonyms: 2510012J08Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212579 representing NM_027381
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGCGCGGGCCGCCAAATGGGTCGGGACTCTCGCTCGCGCTCACGGTCTGTGGGTCGCAGGGGTC
  GGAAGCAGCGGAGTCGAAGCCGGGGCCGGAGCCGCAGCCGGAGTCGGAGTCGGAGTCGGAGCCGGAGCCG
  GAGCCGGAGCCGGAGCCGGAGCCATGGGCGGAGCAGCCGAAGGCGACGGGAACACGAGCGGCGGCGCGAA
  CGCAAGCGGCGCAGCCGAGGGCGCAGGTCAGATTCGGAAGGAGAGCAGCGGCAGAAGTCTAGGAGGCGAA
  GCCAGAGCCTTCGACCACCCCGATGGCACTCACAGAACCAGTCCTCATGCTCTGACTCGGGTGAGGAGCG
  GGCTCAGGGCTCCTGGGCCCGTAAGGGTCACAGGCGCTCGTGGTCCCCGGGGTCATCAGCGTCTAGCTTG
  GACTCCCCAAGACGCTCCCGGAGTCCCGGGACAGCTACCCTGGCCCTGAGCCAGCAGCAGAGCCTGCAGG
  AACGGCTGCGGCTTCGTGAGGAGCGGAAGCAGCAAGAAGAGCTGCTCAAGGCCTTCGAGACGCCTGAGGA
  GAAGCGTGCGCGGCGACTGGCCAAGAAGGAAGCTAAGGAACGCAAGAAGCGGGAGAAGATGGGCTGGGGT
  GAGGAGTACATGGGCTACACCAACACCGACAACCCCTTTGGAGACAACAACCTGCTCGGCACCTTCATCT
  GGAACAAGGCCTTGGAGAAGAAGGGCATCAGCCACCTGGAGGAGAAGGAGCTGAAGGAGCGCAACAAGAG
  GATCCAGGAAGACAACCGTCTGGAGCTGCAAAAGGTCAAACAGCTGCGGCTAGAGCGGGAACGGGAGAAA
  GCCATGAGGGAGCAGGAGCTGGAGTTGCTGCAGCGGGAGAAGGAGGCAGAACACTTTAAGACGTGGGAGG
  AGCAAGAGGACAGCTTCCACTTGCGGCAGGCCAAGCTTCGCTCCAAGATCCGAATCCGAGATGGGCGAGC
  TAAGCCCATCGACCTCCTGGCCAAGTACATCAGCGCAGAGGATGACGACCTGGCGGTGGAGATGCACGAG
  CCCTACACCTTCCTCAACGGCCTCACTGTGGCGGACATGGAGGACCTACTAGAAGACATCCAGGTGTACA
  TGGAACTAGAGCAGGGCAAGAATGTGGACTTCTGGCGGGACATGACGACCATCACAGAGGATGAGATTGC
  CAAGCTCCGCAAGCTGGAGGCCTCTGGCAAGGGCCCAGGTGAGCGCCGTGAGGGAGTCAATGCCTCAGTC
  AGCTCCGATGTGCAGTCGGTGTTCAAGGGAAAGACGTACAACCAGCTGCAGGTCATCTTCCAGGGCATCG
  AGGGCAAGATCCGGGCCGGGGGCCCCAACCTGGACATGGGCTATTGGGAGAGCCTGCTGCAGCAGTTGCG
  TGCACACATGGCCAGGGCCAGGCTCCGTGAGCGCCACCAAGATGTTCTTAGACAGAAGCTGTTCAAGCTG
  AAGCAGGAGCAGGGTGTGGAGAGCGAGCCGCTGTTCCCCATCCTCAAGTCGGAGCCCTCGGCCGCCCACA
  GCCCCGAACCTGAGGAGCGACCCCCCAGCCCCGGGACTTCAGTGGACCCAGTGGAGCCTGTGGAACCTGA
  GGAGGCCACAGCACCAGGAGAAGCAGAGGGGGAGGCAGAAGGGGAGGCAGTGCTGATGGAGGAAGACCTG
  ATCCAGCAGAGCCTGGCGGACTATGACGCCGGCCGCTACAGCCCACGGCTGCTCACGGCACACGAGCTGC
  CTCTGGACGCACACGTGCTGGAGCCTCACGAGGACCTGCAGCGCCTGCAGCTGTCTCGCCAGCAGCTTCA
  GGCTACAGGTGATGCGAGTGAGAGCGCTGAGGACATCTTCTTCCGGCGTGCGAGGGAGGGCATGGGGCAG
  GATGAGGCACAGTTCAGCGTGGAGATGCCACTGGGCGGCCGGGCCTATCTGTGGGCCGACAAGTACCGGC
  CGCGAAAACCTCGCTTCTTCAACCGCGTGCACACGGGCTTCGAGTGGAACAAATATAACCAAACGCATTA
  TGACTTCGACAACCCTCCACCCAAGATTGTGCAGGGCTACAAATTCAACATCTTCTACCCAGACCTCATC
  CGTAAGCGCGCCACACCTGAGTACTTCCTGGAGGCTTGTGCAGACAACCGGGACTTTGCCATTCTGCGCT
  TCCATGCGGGACCGCCCTACGAGGACATCGCCTTCAAGATCGTCAGTCGTGAGTGGGAGTATTCGCACCG
  CCATGGCTTCCGCTGCCAGTTCGCCAATGGAATCTTCCAGCTCTGGTTCCACTTCAAGCGCTACCGCTAC
  CGTCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212579 representing NM_027381
  Red=Cloning site Green=Tags(s)
  
  MRGAGRQMGRDSRSRSRSVGRRGRKQRSRSRGRSRSRSRSRSRSRSRSRSRSRSHGRSSRRRREHERRRE
  RKRRSRGRRSDSEGEQRQKSRRRSQSLRPPRWHSQNQSSCSDSGEERAQGSWARKGHRRSWSPGSSASSL
  DSPRRSRSPGTATLALSQQQSLQERLRLREERKQQEELLKAFETPEEKRARRLAKKEAKERKKREKMGWG
  EEYMGYTNTDNPFGDNNLLGTFIWNKALEKKGISHLEEKELKERNKRIQEDNRLELQKVKQLRLEREREK
  AMREQELELLQREKEAEHFKTWEEQEDSFHLRQAKLRSKIRIRDGRAKPIDLLAKYISAEDDDLAVEMHE
  PYTFLNGLTVADMEDLLEDIQVYMELEQGKNVDFWRDMTTITEDEIAKLRKLEASGKGPGERREGVNASV
  SSDVQSVFKGKTYNQLQVIFQGIEGKIRAGGPNLDMGYWESLLQQLRAHMARARLRERHQDVLRQKLFKL
  KQEQGVESEPLFPILKSEPSAAHSPEPEERPPSPGTSVDPVEPVEPEEATAPGEAEGEAEGEAVLMEEDL
  IQQSLADYDAGRYSPRLLTAHELPLDAHVLEPHEDLQRLQLSRQQLQATGDASESAEDIFFRRAREGMGQ
  DEAQFSVEMPLGGRAYLWADKYRPRKPRFFNRVHTGFEWNKYNQTHYDFDNPPPKIVQGYKFNIFYPDLI
  RKRATPEYFLEACADNRDFAILRFHAGPPYEDIAFKIVSREWEYSHRHGFRCQFANGIFQLWFHFKRYRY
  RR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027381
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027381.2, NP_081657.1
• RefSeq Size: 2724 bp
• RefSeq ORF: 2319 bp
• Locus ID: 70312
• UniProt ID: Q9CS00
• Cytogenetics: 10 C1
• Gene Summary: Involved in the regulation of innate immune response. Acts as negative regulator of Toll-like receptor and interferon-regulatory factor (IRF) signaling pathways. Contributes to the regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous proinflammatory stimuli. May play a role during early embryonic development. Probably involved in pre-mRNA splicing (By similarity).[UniProtKB/Swiss-Prot Function]