Zscan18 (NM_001017955) Mouse Tagged ORF Clone

CAT#: MG212458

Zscan18 (tGFP-tagged) - Mouse zinc finger and SCAN domain containing 18 (Zscan18), (10ug)

Product Images

Circular map for MG212458

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zscan18
• Synonyms: EG232875
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212458 representing NM_001017955
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCTTTGGAGAGGGTGTTAGCCTCTCCTAGAAGCTCTCCACCCCAACCAGAACCATCTACTCTGC
  GGTCAAAAGATACAGTACAGCAGGAAGAACCTGGCCAGCAGGAGGAACCCATCCAGCAGGAAGGACTCAT
  TAAGCAACAAGAACCTAGCCAGCAGGAGGAATGCAGGAGGCAGGATGAACTCATCCGGAAAGAAGAACCC
  ATCCAGCTGCTGGAAACTAGTCAGAGGGAAGAACTCAGTGAGCAGCAAGAACCTAGTCAACAGGATGAGC
  CCAGTCAAGAGGAAGAGTCTGTCCAACTGCAAGAACCCAGCCAGTGGGGAGAACCTGGCCAAAAGGATGT
  ACCCATGCAGCAGGAAGAACCTATGCAGCAGGATGAGCCCAGCCAGTGGGAAGAACCCATGCAGCAAGAT
  GAACCCATGCAGCAGGAAGAACTCATGCAGCAGACAGAGCCCATTGAACAGGATAAGCCCATCCAACAGG
  AAGAACTCACCCAGCAGGAGGAGCCCATTCAGCAGGATGCTTCCATCCAACGTGAACTCATTCATCAGGT
  GAGGCCCATCCAGAAGGAATTGATTCTGCGGGAGGAGTTCATTCAGCAGGATAAGTGCACCCAAAAAGAA
  GAATTTATTCAGCAAGAGAAGCCAATTCAGCAGGTAAGGTTCAGTGAACAGGAGGAACCCAGCCAGCAGG
  AGGAATCCAGCCAGCAGGAGGAATCCAGCCAGCAGGAGGAGCTCAGTCAACAGAAGGAGTCTAGCCAGAA
  GGCTCAACCATACCAACAGGAGGAACCCAGCCAACAGCAAGAGCCTAGCCAGAAGGATCAACCCAGCCAG
  CAGGAGGAACCCAGCCAACAGCAAGAGCCTAGTCAGAAAGATCAACCCAGCCAGCAGGAGGAGCTCAGCC
  AACAGGAAGAGCCTAGCCAGAAGGATCAATGCAACCAACAGGAGGAACCCAGCCAACAACAAGAGCCTAG
  CCAGAAGGATCAACCCAACCAACAGGCAGAACTCAGCCAACAGGAAGAGCCTAGCCAGAAGGATCAATGC
  AACCAACAGGAAGAGCCTAGCCAGAAGGATCAATGCAAACAACAGGAAGAGCCTAGCCAGAAGGATCAAC
  CCAACCAACAGGAAGAGCCTAGCCAGCTGAAGGAAACAGAAACCATCACTGAGAGATCTGCTGCTGACCT
  CGAGTTTTCCCGCCTCCGCTTCCGGGAGTTTGTCTACCAGGAGGCAGCTGGGCCTCACCAGACTTTGGCC
  AGGCTTCATGAGCTATGCCGCCAGTGGCTGAGGCCCGAGGCCTGCTCCAAAGAGCAGATCCTGGAGCTGC
  TCGTTCTGGAGCAGTTCCTGGGCATCTTGCCAGACAGGGTCCGTCCCTGGGTGGTAGCCCAGTATCCCGA
  GAATTGCAAGAAAGCTGCCTCCCTGGTGGAGGGTATCTCTGACATCCTGGAGGAGCCAGGAATGATGTTG
  TGTTCCCCGGGTGGGTCATCATCGTCATTCAGCGAGGGAGACTATGAGAGGTGTCCTGAGCCCCTGCTGC
  TACCATGTGGGTTGCTAAGTCCTAACAGAAACTTAAGACCTAGGGACATCCTGGTACGCCGTGATACTTC
  TCCCCTTCTACCAGCTTGGCCTACTCTGGAATCTGTGCATTTGGATCAAGAGCGTACAGAGGGAGAAAAG
  AGTAAAGAGGCCAACGCTGCCACAGCTGAGCTGAAGGGGCCTCTACAGTCGGAATGGGACCACCTGGATC
  CCACAGAGGATAACCTGACGAGTTACAGCAAGCTGCTCCTGTGGGGGTGTCAGTTTTCCCAGGCTGGTCC
  AACTTCCATGCTGGAGACAGAGGAACCTTGTGCGGTGGAAAACCTGCCAGGGGGCAGCCTCCCAGACAGC
  ACCAGGCAGCAGGAAAGCAAAGGAAATGTGTGTGAGGAAGTCTCCCTGGGGGAGACACTGATGGAGAGTC
  TTTCAGGGGATGTTCCTGTTAGTGCTTCAATAGATACTGCCCTGGAGGAGGAACAGCAACCTCAGAAGGC
  TCTGGATGCCGAGGGTGAAGATTCAAGCCCTGCCAGTCCCCAGAGGCAATCAGTCATCCAGCAGTCAGCC
  CAGGACAAGGATAGGCCACATCAAGGCACTGGGACAAAGAGGCCCCATCCAGATGACGAGGATGAAATGG
  CCCCTGAGTGTCTTTCCAGCACCAGCAGCTACCAGCTGCCATTTGATGCAGGGAAAGTGCTTCATGTGGA
  TGGTCATGATTCTGGGCAGGACTCAGGAACTTCTGCTGGGGGATGCTCTTCAGCTGATAAGTCTGATGCA
  TCTCAAGGGAAGCCCTACACATGCAGTGAATGTGGGGAGGCCTTTGCATGGATCTCCAACCTTATGGAGC
  ATCACAAGAGTCATGGCAGTGAGACATGCTATGTGAGCCAGGGCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212458 representing NM_001017955
  Red=Cloning site Green=Tags(s)
  
  MLPLERVLASPRSSPPQPEPSTLRSKDTVQQEEPGQQEEPIQQEGLIKQQEPSQQEECRRQDELIRKEEP
  IQLLETSQREELSEQQEPSQQDEPSQEEESVQLQEPSQWGEPGQKDVPMQQEEPMQQDEPSQWEEPMQQD
  EPMQQEELMQQTEPIEQDKPIQQEELTQQEEPIQQDASIQRELIHQVRPIQKELILREEFIQQDKCTQKE
  EFIQQEKPIQQVRFSEQEEPSQQEESSQQEESSQQEELSQQKESSQKAQPYQQEEPSQQQEPSQKDQPSQ
  QEEPSQQQEPSQKDQPSQQEELSQQEEPSQKDQCNQQEEPSQQQEPSQKDQPNQQAELSQQEEPSQKDQC
  NQQEEPSQKDQCKQQEEPSQKDQPNQQEEPSQLKETETITERSAADLEFSRLRFREFVYQEAAGPHQTLA
  RLHELCRQWLRPEACSKEQILELLVLEQFLGILPDRVRPWVVAQYPENCKKAASLVEGISDILEEPGMML
  CSPGGSSSSFSEGDYERCPEPLLLPCGLLSPNRNLRPRDILVRRDTSPLLPAWPTLESVHLDQERTEGEK
  SKEANAATAELKGPLQSEWDHLDPTEDNLTSYSKLLLWGCQFSQAGPTSMLETEEPCAVENLPGGSLPDS
  TRQQESKGNVCEEVSLGETLMESLSGDVPVSASIDTALEEEQQPQKALDAEGEDSSPASPQRQSVIQQSA
  QDKDRPHQGTGTKRPHPDDEDEMAPECLSSTSSYQLPFDAGKVLHVDGHDSGQDSGTSAGGCSSADKSDA
  SQGKPYTCSECGEAFAWISNLMEHHKSHGSETCYVSQGC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017955
• ORF Size: 2427 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017955.3
• RefSeq Size: 3626 bp
• RefSeq ORF: 2430 bp
• Locus ID: 232875
• Cytogenetics: 7