Ddb1 (NM_015735) Mouse Tagged ORF Clone

CAT#: MG211698

Ddb1 (tGFP-tagged) - Mouse damage specific DNA binding protein 1 (Ddb1)

Product Images

Circular map for MG211698

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ddb1
• Synonyms: 127kDa; AA408517; p127-Ddb1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211698 representing NM_015735
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTACAACTACGTCGTAACGGCGCAGAAGCCTACTGCAGTGAACGGCTGTGTGACCGGACACTTTA
  CTTCAGCGGAAGATCTGAACCTGTTGATTGCTAAAAACACAAGATTAGAGATCTATGTGGTCACTGCGGA
  GGGTCTTCGGCCAGTCAAAGAGGTGGGAATGTACGGGAAGATTGCAGTCATGGAGCTCTTCAGGCCCAAG
  GGGGAGAGCAAAGACCTGCTGTTCATCTTGACTGCTAAATACAATGCCTGTATCTTGGAGTATAAGCAGA
  GTGGCGAAAGCATTGACATCATTACAAGAGCCCATGGCAATGTCCAGGACCGCATTGGTCGCCCCTCGGA
  GACTGGCATCATTGGCATCATTGACCCTGAGTGCCGTATGATTGGCCTTCGACTCTACGATGGCCTTTTC
  AAGGTCATTCCACTAGACCGAGACAATAAAGAGCTCAAGGCCTTTAACATCCGCCTAGAGGAACTGCACG
  TCATTGATGTCAAGTTCCTGTATGGTTGTCAAGCACCTACCATTTGCTTTGTCTACCAGGATCCTCAGGG
  GCGGCATGTGAAAACCTATGAGGTGTCTCTTCGAGAGAAGGAGTTCAACAAGGGCCCTTGGAAACAGGAG
  AATGTAGAAGCCGAAGCTTCAATGGTGATAGCAGTCCCAGAGCCTTTTGGAGGTGCCATCATCATTGGAC
  AGGAATCAATCACCTATCATAACGGTGATAAATACCTGGCAATTGCTCCTCCTATCATTAAGCAAAGCAC
  TATTGTGTGCCATAACCGGGTGGATCCTAATGGCTCGCGGTACCTTCTGGGAGATATGGAGGGACGGCTT
  TTCATGCTGCTCTTAGAGAAGGAGGAGCAGATGGATGGCACTGTCACCCTCAAGGACCTTCGAGTGGAAC
  TCCTCGGAGAGACCTCCATTGCTGAGTGCCTAACTTATCTTGATAATGGTGTTGTATTTGTCGGGTCTCG
  CCTAGGTGACTCCCAGCTCGTAAAGCTCAATGTCGACAGCAACGAACAAGGCTCCTATGTAGTGGCCATG
  GAAACCTTTACCAATTTAGGACCCATTGTGGATATGTGTGTGGTGGACCTGGAGAGGCAGGGACAGGGTC
  AGCTGGTCACTTGCTCTGGAGCTTTCAAGGAAGGTTCCTTGCGGATCATCCGGAACGGCATTGGAATCCA
  TGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGTTTATGGCCTCTGCGCTCTGACCCCGGTCGGGAG
  ACGGATGACACTTTGGTGCTCTCTTTTGTGGGCCAGACAAGAGTTCTCATGCTAAACGGAGAGGAAGTGG
  AAGAGACAGAACTGATGGGCTTTGTGGACGATCAGCAGACTTTCTTTTGTGGCAACGTGGCTCATCAGCA
  ACTTATCCAGATCACCTCAGCATCTGTGAGGTTGGTGTCTCAAGAGCCCAAAGCTTTGGTCAGCGAGTGG
  AAAGAGCCTCAGGGCAAGAACATCAGTGTGGCCTCCTGTAACAGCAGCCAGGTGGTTGTTGCCGTGGGAA
  GGGCACTATATTACCTTCAGATCCACCCTCAGGAGCTCCGGCAAATCAGCCACACAGAGATGGAACATGA
  AGTGGCATGCTTGGATATCACCCCATTAGGGGACAGCAATGGCCTCTCCCCACTTTGTGCCATTGGACTT
  TGGACAGACATCTCAGCTCGTATCCTGAAGCTCCCATCCTTTGAACTACTACACAAGGAGATGCTGGGTG
  GAGAGATCATTCCTCGATCCATCCTGATGACCACTTTTGAGAGTAGCCACTACCTCCTTTGTGCCTTGGG
  AGATGGGGCTCTTTTCTACTTTGGGCTCAACATCGAGACAGGTTTACTGAGTGACCGTAAAAAGGTGACT
  TTGGGCACCCAGCCCACGGTGTTGAGGACATTCCGTTCTCTTTCTACCACCAACGTCTTTGCTTGCTCTG
  ACCGGCCCACTGTCATCTATAGCAGCAATCACAAGTTGGTCTTCTCCAATGTCAACCTCAAGGAGGTGAA
  CTATATGTGTCCTCTCAACTCAGATGGCTATCCTGACAGTCTGGCATTGGCCAATAACAGCACTCTCACC
  ATTGGCACCATCGATGAGATCCAGAAGCTCCATATTCGCACAGTTCCCCTCTATGAGTCTCCCAGGAAGA
  TCTGCTATCAGGAAGTGTCTCAGTGCTTTGGGGTCCTTTCCAGCCGCATTGAAGTCCAAGATAGCAGTGG
  AGGCACTACTGCTTTGAGGCCCAGTGCCAGCACCCAGGCCCTGTCCAGCAGCGTCAGCTCCAGCAAACTT
  TTCTCCAGCAGTACGGCTCCTCACGAGACCTCCTTTGGAGAAGAAGTGGAGGTGCACAACCTCCTCATCA
  TTGACCAGCACACCTTTGAAGTGCTTCACGCCCATCAGTTTCTACAAAACGAGTATGCCCTCAGCCTGGT
  CTCCTGCAAGTTGGGTAAAGACCCCAACACTTACTTCATTGTGGGCACAGCCATGGTATATCCTGAAGAG
  GCAGAGCCCAAGCAGGGTCGTATTGTGGTCTTCCAGTATTCAGATGGAAAACTACAGACTGTGGCTGAAA
  AGGAAGTAAAAGGAGCTGTGTACTCTATGGTGGAGTTTAATGGGAAGCTGCTTGCCAGCATCAATAGCAC
  GGTGCGGCTCTATGAGTGGACCACAGAAAAGGAGCTTCGCACTGAGTGTAATCACTACAACAACATCATG
  GCCCTGTACCTGAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTCATGCGCTCAGTGCTGCTGCTTG
  CCTACAAGCCTATGGAAGGAAACTTTGAAGAGATTGCCCGAGACTTTAATCCCAACTGGATGAGTGCAGT
  GGAGATCTTGGATGATGACAATTTTCTGGGAGCTGAAAATGCCTTTAACTTGTTTGTGTGTCAGAAGGAC
  AGTGCTGCCACCACTGATGAGGAGCGGCAGCACCTACAGGAGGTTGGTCTCTTCCACCTGGGCGAGTTTG
  TCAATGTGTTCTGCCATGGCTCCCTAGTTATGCAGAATCTGGGCGAGGCTTCTACCCCTACACAGGGCTC
  GGTGCTCTTTGGCACAGTCAACGGCATGATAGGGCTGGTGACCTCGCTGTCAGAGAGCTGGTATAACCTC
  CTGTTGGACATGCAGAACCGACTCAATAAGGTCATCAAAAGCGTGGGCAAGATTGAGCACTCCTTCTGGA
  GATCCTTTCACACTGAGCGAAAGACAGAACCAGCCACAGGCTTCATCGATGGTGATCTGATTGAAAGTTT
  CCTAGATATCAGCCGCCCTAAGATGCAGGAGGTTGTGGCAAACTTGCAGTATGATGATGGCAGTGGTATG
  AAGCGGGAGGCAACTGCAGATGACCTCATCAAAGTCGTGGAGGAGCTAACTCGGATCCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211698 representing NM_015735
  Red=Cloning site Green=Tags(s)
  
  MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPK
  GESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLF
  KVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQE
  NVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRL
  FMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAM
  ETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPGRE
  TDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEW
  KEPQGKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGL
  WTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVT
  LGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLT
  IGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDSSGGTTALRPSASTQALSSSVSSSKL
  FSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEE
  AEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIM
  ALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKD
  SAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGEASTPTQGSVLFGTVNGMIGLVTSLSESWYNL
  LLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGM
  KREATADDLIKVVEELTRIH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015735
• ORF Size: 3420 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015735.2
• RefSeq Size: 4171 bp
• RefSeq ORF: 3423 bp
• Locus ID: 13194
• UniProt ID: Q3U1J4
• Cytogenetics: 19 6.66 cM
• Gene Summary: Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2 (By similarity). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207). DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (PubMed:28790135).[UniProtKB/Swiss-Prot Function]