Ret (BC059012) Mouse Tagged ORF Clone

CAT#: MG211589

Ret (tGFP-tagged) - Mouse ret proto-oncogene (cDNA clone MGC:69638 IMAGE:6849696)

Product Images

Circular map for MG211589

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ret
• Synonyms: RET9, RET51, PTC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211589 representing BC059012
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAAGCGACGTCCGGCGCCGCAGGGCTGGGGCTGAAGCTGATTTTGCTCCTGCCGCTGCTAGGAG
  AAGCCCCACTGGGCCTCTATTTCTCAAGGGATGCTTACTGGGAGAGGCTGTATGTAGACCAGCCAGCTGG
  CACACCTCTGCTCTATGTCCATGCCCTACGGGATGCCCCTGGAGAAGTGCCGAGCTTCCGCCTGGGCCAG
  CATCTCTATGGCGTCTACCGTACACGGCTGCATGAGAATGACTGGATCCGCATCAATGAGACTACTGGCC
  TTCTCTACCTCAATCAGAGCCTGGACCACAGTTCCTGGGAACAGCTCAGCATCCGCAATGGTGGTTTCCC
  CCTGCTCACCATCTTCCTCCAGGTCTTTCTGGGGTCCACAGCCCAGAGAGAGGGAGAATGCCATTGGCCA
  GGCTGTACCCGTGTGTACTTCTCCTTCATCAACGACACCTTCCCAAATTGTAGCTCCTTCAAAGCCCAGG
  ATCTCTGCATCCCAGAGACAGCCGTGTCCTTCCGAGTCAGGGAGAACAGGCCTCCTGGCACCTTCTACCA
  CTTCCACATGTTACCCGTGCAGTTCCTTTGTCCCAACATCAGTGTGAAGTACAGTCTCTTAGGAGGGGAT
  AGTCTGCCCTTCCGTTGTGACCCAGACTGCCTGGAGGTGAGCACTCGCTGGGCCCTGGATCGAGAGCTCC
  GGGAGAAGTATGTGCTGGAGGCTTTGTGCATAGTGGCAGGCCCTGGTGCCAACAAAGAGACGGTGACTCT
  GTCCTTCCCAGTGACAGTGTATGATGAGGACGACTCGGCGCCCACCTTCTCTGGAGGTGTGGGCACTGCC
  AGCGCGGTGGTGGAGTTTAAGCGGAAGGAGGGCACTGTGGTGGCCACCCTGCAGGTGTTCGATGCAGATG
  TGGTGCCAGCGTCTGGGGAGCTGGTGAGACGGTACACAAACACACTCCTCTCAGGGGACTCCTGGGCCCA
  GCAGACCTTCCGGGTGGAGCATTCGCCCATCGAGACCTTGGTCCAGGTCAACAACAACTCCGTTCGGGCA
  ACCATGCACAATTACAAGCTGATTCTCAACAGGAGCCTGTCTATCTCAGAGAGCCGAGTCCTGCAGCTCG
  CGGTCCTGGTCAACGACTCAGACTTCCAGGGGCCTGGGGCAGGTGGTATCCTCGTCCTCCATTTCAACGT
  GTCTGTACTGCCCGTCACCCTGAACCTACCCAGGGCCTACTCCTTCCCAGTGAATAAGAGGGCCCGCCGC
  TATGCCCAGATCGGGAAAGTCTGTGTGGAAAACTGCCAGGAGTTCAGCGGTGTCTCCATCCAGTACAAGC
  TGCAGCCTTCCAGCATCAACTGCACTGCCCTAGGTGTGGTCACCTCACCCGAGGACACCTCGGGGACCCT
  ATTTGTAAATGACACAGAGGCCCTGCGGCGACCTGAGTGCACCAAGCTTCAGTACACGGTGGTAGCCACT
  GACCGGCAGACCCGCAGACAGACCCAGGCTTCGCTAGTGGTCACTGTGGAGGGGACATCCATTACTGAAG
  AAGTAGGCTGCCCCAAGTCCTGTGCAGTAAACAAGAGGCGCCCCGAGTGTGAGGAATGTGGTGGCCTGGG
  TTCTCCAACTGGCAGGTGCGAGTGGCGCCAGGGAGATGGTAAAGGGATCACCAGGAACTTCTCCACCTGC
  TCCCCCAGTACCAGGACCTGCCCCGACGGCCACTGTGATGCTGTGGAGAGCCGGGATGCCAACATTTGCC
  CCCAGGACTGTCTCCGTGCCGACATTGTTGGAGGACACGAGCGAGGGGAGCGCCAGGGCATTAAAGCAGG
  CTACGGCATCTGCAACTGTTTCCCTGATGAGAAGAAATGCTTCTGCGAGCCAGAGGACAGCCAGGGCCCA
  CTGTGTGATGCGCTGTGCCGCACGATCATCACAGCTGCCCTCTTCTCCCTTATCATCTCCATCCTGCTGT
  CCATCTTCTGTGTCTGCCACCACCACAAGCATGGGCACAAGCCGCCCATTGCATCAGCGGAAATGACCTT
  CTGCCGGCCGGCCCAGGGCTTCCCAATCAGTTATTCCTCCTCAGGCACCCGCCGGCCCTCACTGGATTCC
  ACGGAGAACCAGGTTCCTGTGGACTCTTTCAAGATCCCGGAGGATCCAAAGTGGGAATTTCCTCGGAAGA
  ACTTAGTTCTTGGGAAAACTCTGGGAGAAGGCGAGTTTGGAAAAGTTGTCAAGGCCACAGCCTTCCGTCT
  GAAAGGCCGGGCAGGATACACCACAGTGGCTGTGAAAATGCTGAAAGAAAACGCCTCCCAGAGTGAGTTA
  CGAGACCTGCTGTCTGAGTTCAACCTTCTGAAACAAGTCAACCATCCACATGTCATCAAGTTGTATGGGG
  CCTGCAGCCAGGATGGGCCACTTCTTCTCATTGTGGAGTATGCCAAGTATGGCTCCCTGCGGGGATTCCT
  CCGTGACAGCCGCAAGATTGGGCCTGCCTATGTGAGCGGTGGAGGCAGCCGCAACTCCAGCTCGCTGGAC
  CACCCAGATGAAAGGGTACTGACCATGGGTGACCTCATCTCCTTCGCCTGGCAGATCTCGAGGGGTATGC
  AGTACTTGGCAGAAATGAAGCTTGTACATCGGGACTTAGCTGCCAGGAACATCTTGGTGGCTGAGGGACG
  GAAGATGAAGATTTCCGACTTTGGGCTGTCCCGAGATGTTTATGAGGAAGATTCCTATGTGAAGAAAAGC
  AAGGGCCGGATTCCCGTCAAGTGGATGGCAATTGAGTCCCTTTTCGATCACATCTATACTACTCAAAGTG
  ATGTGTGGTCCTTTGGAGTGCTGCTCTGGGAGATTGTGACCCTGGGAGGCAACCCCTACCCTGGAATTCC
  TCCTGAACGACTCTTCAACCTTCTGAAGACAGGCCACAGGATGGAGAGGCCAGACAACTGCAGCGAGGAA
  ATGTACCGTCTGATGCTGCAGTGCTGGAAGCAGGAGCCAGACAAGAGGCCAGTGTTTGCTGACATCAGCA
  AGGATCTGGAGAAGATGATGGTCAAGAGCAGAGACTACTTGGACCTGGCTGCATCCACACCTTCGGACTC
  ACTGCTGTATGACGATGGGCTCTCAGAAGAGGAGACACCCCTGGTGGACTGTAACAATGCTCCCCTCCCG
  CGCTCCCTCCCTTCCACATGGATTGAAAACAAACTCTATGGTAGAATTTCACATGCATTTACTAGATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211589 representing BC059012
  Red=Cloning site Green=Tags(s)
  
  MAKATSGAAGLGLKLILLLPLLGEAPLGLYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQ
  HLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWP
  GCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSFRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGD
  SLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTA
  SAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRA
  TMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARR
  YAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVAT
  DRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTC
  SPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGP
  LCDALCRTIITAALFSLIISILLSIFCVCHHHKHGHKPPIASAEMTFCRPAQGFPISYSSSGTRRPSLDS
  TENQVPVDSFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSEL
  RDLLSEFNLLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRDSRKIGPAYVSGGGSRNSSSLD
  HPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKKS
  KGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEE
  MYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNNAPLP
  RSLPSTWIENKLYGRISHAFTRF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC059012
• ORF Size: 3221 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059012, AAH59012
• RefSeq Size: 4517 bp
• RefSeq ORF: 3221 bp
• Locus ID: 19713
• Cytogenetics: 6 55.86 cM
• Gene Summary: Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which induces inhibition of food-intake. Activates MAPK- and AKT-signaling pathways (PubMed:28846099).[UniProtKB/Swiss-Prot Function]