Vps35 (BC005469) Mouse Tagged ORF Clone

CAT#: MG210707

Vps35 (tGFP-tagged) - Mouse vacuolar protein sorting 35 (cDNA clone MGC:6633 IMAGE:3492842)

Product Images

Circular map for MG210707

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vps35
• Synonyms: AI647796; Mem3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210707 representing BC005469
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTACAACACAGCAGTCACCCCAGGATGAGCAGGAAAAACTTTTGGATGAAGCCATCCAGGCTGTGA
  AGGTTCAGTCATTCCAGATGAAAAGATGCCTGGACAAAAACAAGCTGATGGATGCTCTGAAGCATGCCTC
  CAATATGCTTGGAGAGCTCCGGACCTCTATGCTGTCACCAAAGAGTTACTATGAACTTTATATGGCTATT
  TCTGATGAACTGCACTACTTGGAGGTCTACCTGACTGATGAATTTGCTAAAGGAAGAAAGGTGGCAGATC
  TCTATGAACTTGTACAGTACGCGGGAAACATTATTCCAAGGCTTTATCTCTTGATCACAGTTGGAGTTGT
  ATATGTCAAGTCATTTCCTCAATCCAGGAAAGATATTTTGAAAGATTTGGTAGAAATGTGCCGTGGTGTG
  CAGCATCCGCTAAGGGGTTTGTTTCTTCGAAATTACCTTCTTCAGTGTACTAGGAACATTTTACCTGATG
  AAGGAGAGCCAACAGATGAAGAAACAACTGGTGATATCAGTGATTCCATGGATTTTGTACTACTCAACTT
  TGCAGAAATGAATAAGCTCTGGGTGCGGATGCAGCATCAAGGACATAGTCGAGATAGAGAAAAAAGAGAA
  CGAGAGAGACAAGAACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTTAGTCAGTTGGAAGGTGTAA
  ATGTGGAACGTTACAAACAGATTGTTCTAACAGGCATTCTGGAGCAGGTTGTGAATTGTAGGGATGCTCT
  GGCTCAGGAGTATCTCATGGAGTGCATCATTCAGGTTTTTCCTGATGAGTTTCATCTTCAGACTTTGAAT
  CCTTTTCTTAGAGCTTGTGCTGAATTACACCAAAATGTAAATGTGAAAAACATAATCATTGCTTTAATTG
  ACAGATTAGCTTTATTTGCTCATCGTGAAGATGGACCCGGAATTCCAGCTGAGATTAAACTTTTTGACAT
  ATTTTCACAACAGGTGGCTACAGTGATACAGTCCAGACAAGACATGCCATCAGAGGATGTTGTATCTTTA
  CAAGTCTCTCTCATTAATCTTGCTATGAAATGTTACCCTGATCGTGTGGACTACGTCGATAAAGTTCTAG
  AAACAACAGTGGAGATATTCAATAAGCTTAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGA
  GCTTACCAGACTTTTGAAGATACCTGTTGATACTTACAACAATATCTTAACAGTATTAAAGTTAAAGCAT
  TTCCACCCACTTTTTGAGTACTTTGACTACGAGTCCAGAAAGAGCATGAGCTGTTATGTGCTTAGTAATG
  TTCTGGATTATAACACAGAAATCGTCTCTCAGGACCAGGTAGATTCCATAATGAATTTGGTGTCCACATT
  GATTCAGGATCAGCCAGACCAACCTGTAGAAGACCCTGATCCAGAAGACTTCGCTGATGAACAGAGCCTT
  GTTGGCCGATTTATTCATCTGCTACGTTCTGATGATCCTGACCAGCAGTATTTGATTTTGAATACGGCAC
  GAAAACATTTTGGGGCTGGTGGAAATCAGCGGATTCGCTTCACACTGCCACCTTTGGTATTTGCAGCTTA
  CCAGTTGGCTTTTCGATACAAAGAGAATTCCCAAATGGATGACAAGTGGGAAAAGAAATGCCAGAAGATA
  TTTTCATTTGCTCACCAGACTATCAGTGCTTTGATTAAAGCTGAGCTGGCTGAATTACCACTGAGACTTT
  TTCTTCAAGGAGCATTAGCTGCTGGAGAAATTGGCTTTGAAAATCATGAAACAGTAGCATATGAATTTAT
  GTCCCAGGCATTTTCTCTATATGAAGATGAAATCAGTGATTCTAAAGCACAGCTGGCTGCTATCACCTTG
  ATCATCGGTACTTTTGAGAGGATGAAATGCTTCAGTGAAGAGAATCATGAACCCTTGAGAACTCAGTGTG
  CACTTGCTGCATCAAAACTTCTGAAAAAACCAGATCAAGGCCGAGCCGTGAGCACATGTGCACATCTCTT
  TTGGTCTGGCCGAAACACAGACAAAAATGGGGAAGAGCTTCATGGAGGTAAAAGGGTCATGGAGTGCCTA
  AAGAAGGCACTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTTCAGCTCTTTATAGAGATTC
  TGAACAGGTACATCTATTTCTATGAAAAAGAAAATGATGCGGTAACCATTCAAGTCTTGAACCAACTTAT
  TCAAAAGATTCGAGAAGATCTCCCAAATCTTGAGTCCAGTGAAGAAACAGAACAAATAAACAAGCATTTT
  CACAACACATTGGAGCACTTGCGCTCAAGACGGGAATCACCAGAGTCTGAGGGGCCAATCTATGAAGGTC
  TCATCCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210707 representing BC005469
  Red=Cloning site Green=Tags(s)
  
  MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAI
  SDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGV
  QHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRE
  RERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLN
  PFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSL
  QVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKH
  FHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSL
  VGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSQMDDKWEKKCQKI
  FSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITL
  IIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECL
  KKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHF
  HNTLEHLRSRRESPESEGPIYEGLIL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005469
• ORF Size: 2390 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005469, AAH05469
• RefSeq Size: 3295 bp
• RefSeq ORF: 2390 bp
• Locus ID: 65114
• Cytogenetics: 8 41.61 cM
• Gene Summary: Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of WASHC2 and mediates the association of the CSC with the WASH complex (By similarity).[UniProtKB/Swiss-Prot Function]