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Plekhg6 (NM_198604) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210656
Plekhg6 (tGFP-tagged) - Mouse pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (Plekhg6)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $921.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Plekhg6
Synonyms BC026778; Gm155
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210656 representing NM_198604
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAGCCTTTGGTCCTCCAAATGAGGGCCCCCTCCAAGGCCTGGTAGCTTCCCGAATTGAGACTTACG
GGGGCCGTCATCAGACCTCTGCCCACAGCACTGCTGGGAATCTATTTCCCAGAGGAGGTCCTGGAGTGGA
CCCTAGTCGCCGACGCCTTCAGCACTATGTCCCCTTTGCCAAGGGCTCTGGCCAGACCCGAGGCATGTCT
CCTCTGGTGCTTCGGGAACCAGACCCAGAGAAGAGGCATGGAAGCTATTTTGGGGTCGGCCCACCTCACT
CCCCCAAACTCAAGGAAGTCACCAGGGCCCATGAGCTAGAAATAAGGCTGCACACATTCAGCATGTTTGG
GATGCCCCGCCTGCCACCAGAGGACCGGAGACACTGGGAGATAGGAGAGGGAGGCGATAGTGCCCTGACC
ATGGAAAAGTCCTGGAAGGAGCTGGTGCTGGAACACAAGGAGATGAACCGCCAGCTTTGCCACCAGCAGG
AAGCACTGTGGGAACTCCTGACCACTGAGCTGATCTACCTGAGAAAGCTCAAGATCATGACTGATCTCCT
GGCAGCTGGTCTGCTGAATTTGCAGCGGGTGGGGCTGCTGACTGAGGTGTCGGCTGAGACCCTGTTTGGA
AATGTCCCCAACCTGATTAGAGCCCACCGGAGATTTTGGGAGGAGGTGCTGCAGCCCATCCTGGAAGACA
CTCGCACCTCTGGCCAACCTTTGGATCCTGTCAGCTTACAAAATGGCTTCCTGACATTTGGCCAGCGGTT
CCAACCTTACGTCCAGTACTGCCTTCGAGTAAAGCAGACCATGGCTTATGCGCGGGAGCAGCAAGACACG
AACCCACTGTTCCACACCTTCGTGCAGTGGTGTGAGAAACACAAGCGCTCCGGAAGACAGACGCTGGGTG
ACTTGCTCATCAAGCCCCACCAGCGCATCACCAAATACCCACTGCTGCTGCAGGCTGTGCGCAAGAGAAG
CCCCGAGCCCCGAGCCCAAGAGGCCCTGAATGCCATGATCGAGGCTGTGGAGTCCTTCCTGCGACACATA
AATGGCCAGGTCCGACAGGGTGAAGAGCAGGAGAGTTTGATGGCTGTGGCCCAGCGCATAGGACCCTATG
AGGTGCTAGAGCCCTCCAGCGAGGAGGTGGAGAAGAACCTGCGTCCATTCTCCACCCTGGATCTGATGAC
TCCTGTACTAGGGGTTGCCCCTGAGTATACCAGGCAGCTGCTGCTGGAAGGACCTGTGCGGGTGAAGGAG
GGACGAGAAGGGAAGATGGATGTGTACCTATTTCTCTTCTCCGATGTGCTCCTGGTAACCAAACCTCAAC
GCAAGGCGGACAGAGCCAAGGTCATCCGTCCTCCCCTCATGCTGGAGAAACTCGTGTGCCGACCACTGCG
AGACCCCAACAGCTTCCTGTTGATCCACCTCACAGAATTCCAGTGTGTCTCCAGTGCCCTCACTGTGCAC
TGTCCCAGTTCTACAGAGCGTGCACGGTGGCTGGAGAAGACCCAGCATGCTCAGACCACTCTGCAGAAGC
TGAAGGGTGAGCAGTACATTCAACAGAAGAGGGAGCTGCTGGCCCTCTATCGCAACCAGGGCACGGAGTC
CCCCAGCACCAGGCCCTCCACACCTTCGCCTTCCCCGGAGGACTCCCAGAGCAGTGCCGAGGGGAGGACT
TTAGAGTTCGCCATCATCCCCCGCCTGGTGGTGACTGAGGATACAGATGAAGACACGCCCTCCATGCCGG
ATGACGCCTCAGACTCTGGCTACGGTACCTTGATTCCAAGCTCCCCGAAGGATTCTCATTCCCCCCTGAA
CAGGCTTCGGTCCAAGGCTCTTCGTAGAGACCCTCGCCTCACCTTCTCCACCCTGGAACTCAGAGACGTC
CCCTTGCGCCCCCAGCCTCCCGACCCCCAAGCTCCCCAGCGGCGAAGCGCCCCAGAGTTGCCAGAAGGGA
TCCTAAGAGGAGGCAGTCTACCCAGGAGAGCCCCACCAATCTGGTCGGAGGAGGAAGACGAGACTTTGGC
ATCAGGGAATGTGGTGGTAGAAACCCTACACAGGGCGCAACGTCGGAGTCCGCTCCCCCACTCTCCCACT
CATACTGACTCTGCTGGGGAAAGCCCCTGGGAGTCCTCAGATGAAGATGAGGGGCTTCTATCCCCTGAAC
TCCGCCCCCGCTCCCTACGGGAGGACATGCTCAGGGAGATCAGGGAGGAGCTGGCCAACCAAAGGATCGA
TGGAGCCTCGGAACCGGAACCAGGGAATGGCAAGCCCCGGAGGCTGACTCTAGCCCAGCTGCAGAGAATG
AGGGTCCCCCACATCATACAGCTGGACACGCCCCTGTCTACATCAGAAGTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210656 representing NM_198604
Red=Cloning site Green=Tags(s)

MQAFGPPNEGPLQGLVASRIETYGGRHQTSAHSTAGNLFPRGGPGVDPSRRRLQHYVPFAKGSGQTRGMS
PLVLREPDPEKRHGSYFGVGPPHSPKLKEVTRAHELEIRLHTFSMFGMPRLPPEDRRHWEIGEGGDSALT
MEKSWKELVLEHKEMNRQLCHQQEALWELLTTELIYLRKLKIMTDLLAAGLLNLQRVGLLTEVSAETLFG
NVPNLIRAHRRFWEEVLQPILEDTRTSGQPLDPVSLQNGFLTFGQRFQPYVQYCLRVKQTMAYAREQQDT
NPLFHTFVQWCEKHKRSGRQTLGDLLIKPHQRITKYPLLLQAVRKRSPEPRAQEALNAMIEAVESFLRHI
NGQVRQGEEQESLMAVAQRIGPYEVLEPSSEEVEKNLRPFSTLDLMTPVLGVAPEYTRQLLLEGPVRVKE
GREGKMDVYLFLFSDVLLVTKPQRKADRAKVIRPPLMLEKLVCRPLRDPNSFLLIHLTEFQCVSSALTVH
CPSSTERARWLEKTQHAQTTLQKLKGEQYIQQKRELLALYRNQGTESPSTRPSTPSPSPEDSQSSAEGRT
LEFAIIPRLVVTEDTDEDTPSMPDDASDSGYGTLIPSSPKDSHSPLNRLRSKALRRDPRLTFSTLELRDV
PLRPQPPDPQAPQRRSAPELPEGILRGGSLPRRAPPIWSEEEDETLASGNVVVETLHRAQRRSPLPHSPT
HTDSAGESPWESSDEDEGLLSPELRPRSLREDMLREIREELANQRIDGASEPEPGNGKPRRLTLAQLQRM
RVPHIIQLDTPLSTSEV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198604
ORF Size 2361 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198604.2, NP_941006.2
RefSeq Size 3019 bp
RefSeq ORF 2364 bp
Locus ID 213522
UniProt ID Q8R0J1
Cytogenetics 6 F3
Summary Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198604" in other vectors (4)
SKU Description Size Price
MC204061 Plekhg6 (untagged) - Mouse pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (Plekhg6), (10ug) 10 ug
$722.00
MR210656 Plekhg6 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (Plekhg6) 10 ug
$589.00 $721.00
MR210656L3 Lenti ORF clone of Plekhg6 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (Plekhg6) 10 ug
$1,021.00
MR210656L4 Lenti ORF clone of Plekhg6 (mGFP-tagged) - Mouse pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (Plekhg6) 10 ug
$1,021.00

Plasmid Map for MG210656

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210656
Circular map for MG210656

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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