Flrt2 (BC096471) Mouse Tagged ORF Clone

CAT#: MG209836

Flrt2 (tGFP-tagged) - Mouse fibronectin leucine rich transmembrane protein 2 (cDNA clone MGC:106049 IMAGE:3582155)

Product Images

Circular map for MG209836

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Flrt2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209836 representing BC096471
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCTACAGACTACAAAGTGGCCCGGCCGTGGGGCTTTCATCCTCAAATTTTGGCTCATCATTTCCC
  TGGGACTCTACTTACAAGTGTCAAAACTCCTGGCCTGCCCTAGTGTATGCCGCTGTGACAGGAACTTTGT
  CTACTGTAACGAGAGAAGCTTGACCTCAGTGCCTCTTGGGATCCCGGAGGGCGTAACCGTACTCTACCTC
  CACAACAACCAAATTAATAATGCTGGCTTTCCTGCAGAGCTGCACAATGTCCAGTCAGTGCACACTGTCT
  ACCTTTATGGCAACCAGTTGGATGAGTTCCCCATGAACCTTCCCAAGAATGTCAGAGTTCTCCATCTGCA
  GGAAAACAACATTCAGACCATCTCGCGGGCTGCTCTCGCTCAGCTCCTCAAGCTGGAAGAACTCCACCTG
  GACGACAACTCCATATCTACAGTGGGAGTAGAAGACGGAGCGTTCCGGGAGGCGATTAGCCTCAAACTGT
  TGTTTTTATCGAAGAATCACCTGAGTAGCGTGCCTGTTGGGCTTCCTGTAGACTTGCAAGAGCTGAGAGT
  GGATGAAAACCGAATTGCCGTCATATCAGACATGGCCTTTCAGAACCTCACAAGCTTGGAGCGCCTGATC
  GTGGATGGGAATCTTCTGACCAACAAGGGCATTGCTGAGGGTACCTTCAGTCATCTCACCAAGCTCAAGG
  AATTTTCTATAGTCCGGAACTCGCTCTCCCACCCACCTCCTGATCTCCCAGGTACGCATCTGATCAGGCT
  CTACTTGCAGGATAACCAGATAAACCATATCCCATTGACAGCCTTCGCAAACCTCCGTAAGCTGGAAAGG
  CTAGATATATCCAACAACCAGCTACGAATGTTAACTCAAGGAGTCTTCGATCATCTCTCCAACCTGAAAC
  AACTCACTGCGCGGAATAACCCTTGGTTTTGTGACTGCAGCATTAAATGGGTCACAGAATGGCTCAAGTA
  TATCCCTTCTTCTCTCAACGTGCGTGGTTTCATGTGCCAAGGTCCTGAGCAAGTCCGCGGGATGGCTGTC
  AGGGAGTTGAATATGAATCTTTTGTCTTGTCCCACCACGACTCCTGGCCTACCTGTCTTTACCCCAGCTC
  CAAGTACCGTTTCTCCAACAACTCAGTCTCCTACTCTCTCTGTTCCAAGCCCCAGCAGAGGCTCTGTGCC
  TCCAGCTCCTACCCCATCGAAACTTCCCACCATCCCTGATTGGGATGGCAGAGAAAGAGTGACCCCACCT
  ATTTCTGAAAGGATCCAACTTTCCATCCACTTTGTTAATGATACTTCGATTCAAGTCAGCTGGCTGTCTC
  TCTTTACTGTGATGGCCTACAAACTGACATGGGTGAAAATGGGCCACAGTCTCGTAGGGGGCATCGTTCA
  GGAACGAATTGTCAGTGGTGAGAAGCAACACCTGAGCTTGGTTAATTTAGAGCCCAGATCCACGTATAGG
  ATTTGTTTAGTGCCGCTGGATGCGTTCAACTACCGCACTGTGGAAGATACCATCTGTTCGGAGGCTACCA
  CCCATGCCTCTTATTTGAACAACGGCAGCAACACTGCTTCTAGCCATGAGCAGACGACTTCCCACAGTAT
  GGGCTCCCCTTTTCTGCTCGCAGGCTTGATTGGGGGCGCAGTGATTTTTGTGCTCGTTGTCTTGCTCAGC
  GTCTTTTGCTGGCACATGCACAAAAAGGGACGCTACACCTCTCAGAAGTGGAAATACAACCGGGGCCGAC
  GGAAAGACGACTATTGTGAAGCGGGTACCAAAAAAGACAACTCCATCTTGGAGATGACAGAAACAAGTTT
  TCAGATTGTCTCCTTAAATAACGATCAGCTCCTTAAAGGAGATTTCAGACTGCAGCCCATTTATACCCCA
  AATGGGGGCATTAATTACACAGACTGCCACATCCCCAACAACATGAGATACTGCAACAGCAGTGTGCCAG
  ATCTAGAGCATTGCCATACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209836 representing BC096471
  Red=Cloning site Green=Tags(s)
  
  MGLQTTKWPGRGAFILKFWLIISLGLYLQVSKLLACPSVCRCDRNFVYCNERSLTSVPLGIPEGVTVLYL
  HNNQINNAGFPAELHNVQSVHTVYLYGNQLDEFPMNLPKNVRVLHLQENNIQTISRAALAQLLKLEELHL
  DDNSISTVGVEDGAFREAISLKLLFLSKNHLSSVPVGLPVDLQELRVDENRIAVISDMAFQNLTSLERLI
  VDGNLLTNKGIAEGTFSHLTKLKEFSIVRNSLSHPPPDLPGTHLIRLYLQDNQINHIPLTAFANLRKLER
  LDISNNQLRMLTQGVFDHLSNLKQLTARNNPWFCDCSIKWVTEWLKYIPSSLNVRGFMCQGPEQVRGMAV
  RELNMNLLSCPTTTPGLPVFTPAPSTVSPTTQSPTLSVPSPSRGSVPPAPTPSKLPTIPDWDGRERVTPP
  ISERIQLSIHFVNDTSIQVSWLSLFTVMAYKLTWVKMGHSLVGGIVQERIVSGEKQHLSLVNLEPRSTYR
  ICLVPLDAFNYRTVEDTICSEATTHASYLNNGSNTASSHEQTTSHSMGSPFLLAGLIGGAVIFVLVVLLS
  VFCWHMHKKGRYTSQKWKYNRGRRKDDYCEAGTKKDNSILEMTETSFQIVSLNNDQLLKGDFRLQPIYTP
  NGGINYTDCHIPNNMRYCNSSVPDLEHCHT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC096471
• ORF Size: 1982 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC096471, AAH96471
• RefSeq Size: 2968 bp
• RefSeq ORF: 1982 bp
• Locus ID: 399558
• Cytogenetics: 12 E
• Gene Summary: Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:21350012, PubMed:25728924 PubMed:25374360). May play a role in the migration of cortical neurons during brain development via its interaction with UNC5D (PubMed:21673655). Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5D, and possibly also other UNC-5 family members (PubMed:21673655, PubMed:25728924). Plays a role in fibroblast growth factor-mediated signaling cascades (PubMed:16872596). Required for normal organization of the cardiac basement membrane during embryogenesis, and for normal embryonic epicardium and heart morphogenesis (PubMed:21350012).[UniProtKB/Swiss-Prot Function]