Egfr (BC023729) Mouse Tagged ORF Clone

CAT#: MG209803

Egfr (tGFP-tagged) - Mouse epidermal growth factor receptor (cDNA clone MGC:36101 IMAGE:5351807)

Product Images

Circular map for MG209803

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Egfr
• Synonyms: 9030024J15Rik; AI552599; Erbb; Errb1; Errp; wa-2; wa2; Wa5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209803 representing BC023729
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACCCTCAGGGACCGCGAGAACCACACTGCTGGTGTTGCTGACCGCGCTCTGCGCCGCAGGTGGGG
  CGTTGGAGGAAAAGAAAGTCTGCCAAGGCACAAGTAACAGGCTCACCCAACTGGGCACTTTTGAAGACCA
  CTTTCTGAGCCTGCAGAGGATGTACAACAACTGTGAAGTGGTCCTTGGGAACTTGGAAATTACCTATGTG
  CAAAGGAATTACGACCTTTCCTTCTTAAAGACCATCCAGGAGGTGGCCGGCTATGTCCTCATTGCCCTCA
  ACACCGTGGAGAGAATCCCTTTGGAGAACCTGCAGATCATCAGGGGAAATGCTCTTTATGAAAACACCTA
  TGCCTTAGCCATCCTGTCCAACTATGGGACAAACAGAACTGGGCTTAGGGAACTGCCCATGCGGAACTTA
  CAGGAAATCCTGATTGGTGCTGTGCGATTCAGCAACAACCCCATCCTCTGCAATATGGATACTATCCAGT
  GGAGGGACATCGTCCAAAACGTCTTTATGAGCAACATGTCAATGGACTTACAGAGCCATCCGAGCAGTTG
  CCCCAAATGTGATCCAAGCTGTCCCAATGGAAGCTGCTGGGGAGGAGGAGAGGAGAACTGCCAGAAATTG
  ACCAAAATCATCTGTGCCCAGCAATGTTCCCATCGCTGTCGTGGCAGGTCCCCCAGTGACTGCTGCCACA
  ACCAATGTGCTGCGGGGTGTACAGGGCCCCGAGAGAGTGACTGTCTGGTCTGCCAAAAGTTCCAAGATGA
  GGCCACATGCAAAGACACCTGCCCACCACTCATGCTGTACAACCCCACCACCTATCAGATGGATGTCAAC
  CCTGAAGGGAAGTACAGCTTTGGTGCCACCTGTGTGAAGAAGTGCCCCCGAAACTACGTGGTGACAGATC
  ATGGCTCATGTGTCCGAGCCTGTGGGCCTGACTACTACGAAGTGGAAGAAGATGGCATCCGCAAGTGTAA
  AAAATGTGATGGGCCCTGTCGCAAAGTTTGTAATGGCATAGGCATTGGTGAATTTAAAGACACACTCTCC
  ATAAATGCTACAAACATCAAACACTTCAAATACTGCACTGCCATCAGCGGGGACCTTCACATCCTGCCAG
  TGGCCTTTAAGGGGGATTCTTTCACGCGCACTCCTCCTCTAGACCCACGAGAACTAGAAATTCTAAAAAC
  CGTAAAGGAAATAACAGGCTTTTTGCTGATTCAGGCTTGGCCTGATAACTGGACTGACCTCCATGCTTTC
  GAGAACCTAGAAATAATACGTGGCAGAACAAAGCAACATGGTCAGTTTTCTTTGGCGGTCGTTGGCCTGA
  ACATCACATCACTGGGGCTGCGTTCCCTCAAGGAGATCAGTGATGGGGATGTGATCATTTCTGGAAACCG
  AAATTTGTGCTACGCAAACACAATAAACTGGAAAAAACTCTTCGGGACACCCAATCAGAAAACCAAAATC
  ATGAACAACAGAGCTGAGAAAGACTGCAAGGCCGTGAACCACGTCTGCAATCCTTTATGCTCCTCGGAAG
  GCTGCTGGGGCCCTGAGCCCAGGGACTGTGTCTCCTGCCAGAATGTGAGCAGAGGCAGGGAGTGCGTGGA
  GAAATGCAACATCCTGGAGGGGGAACCAAGGGAGTTTGTGGAAAATTCTGAATGCATCCAGTGCCATCCA
  GAATGTCTGCCCCAGGCCATGAACATCACCTGTACAGGCAGGGGACCAGACAACTGCATCCAGTGTGCCC
  ACTACATTGATGGCCCACACTGTGTCAAGACCTGCCCAGCTGGCATCATGGGAGAGAACAACACTCTGGT
  CTGGAAGTATGCAGATGCCAATAATGTCTGCCACCTATGCCACGCCAACTGTACCTATGGATGTGCTGGG
  CCAGGTCTTCAAGGATGTGAAGTGTGGCCATCTGGGTACGTTCAATGGCAGTGGATCTTAAAGACCTTTT
  GGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209803 representing BC023729
  Red=Cloning site Green=Tags(s)
  
  MRPSGTARTTLLVLLTALCAAGGALEEKKVCQGTSNRLTQLGTFEDHFLSLQRMYNNCEVVLGNLEITYV
  QRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAILSNYGTNRTGLRELPMRNL
  QEILIGAVRFSNNPILCNMDTIQWRDIVQNVFMSNMSMDLQSHPSSCPKCDPSCPNGSCWGGGEENCQKL
  TKIICAQQCSHRCRGRSPSDCCHNQCAAGCTGPRESDCLVCQKFQDEATCKDTCPPLMLYNPTTYQMDVN
  PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGIRKCKKCDGPCRKVCNGIGIGEFKDTLS
  INATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPDNWTDLHAF
  ENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKI
  MNNRAEKDCKAVNHVCNPLCSSEGCWGPEPRDCVSCQNVSRGRECVEKCNILEGEPREFVENSECIQCHP
  ECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGIMGENNTLVWKYADANNVCHLCHANCTYGCAG
  PGLQGCEVWPSGYVQWQWILKTFWI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC023729
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023729, AAH23729
• RefSeq Size: 2666 bp
• RefSeq ORF: 1967 bp
• Locus ID: 13649
• Cytogenetics: 11 9.41 cM
• Gene Summary: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Plays a role in enhancing learning and memory performance (PubMed:20639532).[UniProtKB/Swiss-Prot Function]