Gusb (NM_010368) Mouse Tagged ORF Clone

CAT#: MG209741

Gusb (tGFP-tagged) - Mouse glucuronidase, beta (Gusb)

Product Images

Circular map for MG209741

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gusb
• Synonyms: AI747421; asd; g; Gur; Gus; Gus-r; Gus-s; Gus-t; Gus-u; Gut
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209741 representing NM_010368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTAAAATGGAGTGCGTGTTGGGTCGCGCTGGGCCAGCTGCTGTGCAGCTGCGCGCTGGCTCTGA
  AGGGCGGGATGCTGTTCCCGAAGGAGAGCCCGTCGCGGGAGCTCAAGGCGCTGGACGGACTGTGGCACTT
  CCGCGCCGACCTCTCGAACAACCGGCTGCAGGGTTTCGAGCAGCAATGGTACCGGCAGCCGCTACGGGAG
  TCGGGCCCAGTCTTGGACATGCCTGTCCCTTCTAGCTTCAATGACATCACCCAAGAAGCAGCCCTTCGGG
  ACTTTATTGGCTGGGTGTGGTATGAACGGGAAGCAATCCTGCCACGGCGATGGACCCAAGATACCGACAT
  GAGAGTGGTGTTGAGGATCAACAGTGCCCATTATTATGCAGTTGTGTGGGTGAATGGGATTCATGTGGTG
  GAACATGAGGGAGGTCACCTCCCCTTTGAGGCTGACATTAGCAAGCTGGTCCAGAGTGGGCCCCTGACCA
  CCTGCCGGATCACGATTGCCATCAACAACACACTGACCCCTCATACCCTTCCGCCGGGGACCATCGTCTA
  CAAGACTGACACCTCCATGTATCCCAAGGGTTACTTTGTCCAGGACACAAGCTTTGACTTCTTCAACTAT
  GCGGGACTGCATCGATCTGTGGTCCTCTATACCACCCCTACCACTTACATCGATGATATCACTGTGATCA
  CTAATGTGGAGCAAGACATCGGGCTGGTGACCTACTGGATTTCTGTGCAGGGCAGTGAACATTTCCAGCT
  AGAAGTGCAACTTTTGGATGAGGGTGGCAAAGTCGTGGCCCATGGGACAGGGAACCAGGGTCAACTTCAG
  GTTCCCAGTGCCAACCTCTGGTGGCCTTACCTGATGCATGAGCATCCAGCCTACATGTACTCCTTGGAGG
  TGAAGGTGACAACAACTGAGTCTGTGACTGACTACTACACCCTTCCTATCGGGATTCGAACAGTGGCTGT
  CACAAAGAGCAAGTTCCTCATAAACGGGAAGCCCTTCTATTTCCAAGGGGTCAATAAGCACGAGGATTCA
  GATATCCGAGGGAAAGGCTTCGACTGGCCGCTGCTGGTAAAGGATTTCAACCTGCTCCGTTGGCTCGGGG
  CAAATTCCTTTCGTACCAGCCACTATCCCTACTCAGAGGAGGTACTTCAGCTCTGTGACCGATACGGGAT
  TGTGGTCATCGATGAGTGTCCCGGTGTGGGCATTGTGCTACCTCAGAGTTTTGGCAACGAGTCACTTCGG
  CACCACCTAGAGGTGATGGAGGAGCTGGTTCGCCGGGACAAAAATCACCCTGCGGTTGTGATGTGGTCTG
  TGGCCAATGAGCCTTCCTCTGCTCTGAAACCCGCCGCATATTACTTTAAGACGCTGATCACCCACACCAA
  AGCCCTGGACCTCACCCGTCCCGTGACCTTTGTGAGCAACGCCAAATATGATGCAGACCTGGGGGCCCCG
  TACGTGGATGTTATCTGTGTAAACAGCTACTTTTCTTGGTATCATGACTATGGGCATTTGGAGGTGATTC
  AGCCACAGCTGAATAGCCAGTTTGAGAACTGGTATAAGACGCATCAGAAGCCGATTATCCAGAGCGAGTA
  TGGAGCAGACGCAATCCCAGGGATCCACGAGGACCCGCCTCGCATGTTCAGTGAGGAGTACCAGAAGGCT
  GTTCTGGAGAATTACCATTCAGTTCTGGATCAGAAACGTAAAGAATACGTGGTCGGAGAGCTCATCTGGA
  ATTTCGCCGACTTCATGACGAACCAGTCACCGCTGAGAGTAATCGGAAACAAGAAGGGGATCTTCACTCG
  CCAGAGACAGCCCAAAACTTCGGCCTTTATTTTGCGAGAGAGATACTGGAGGATTGCCAACGAAACCGGA
  GGTCACGGTTCAGGGCCGAGAACCCAGTGTTTCGGAAGCAGACCGTTCACGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209741 representing NM_010368
  Red=Cloning site Green=Tags(s)
  
  MSLKWSACWVALGQLLCSCALALKGGMLFPKESPSRELKALDGLWHFRADLSNNRLQGFEQQWYRQPLRE
  SGPVLDMPVPSSFNDITQEAALRDFIGWVWYEREAILPRRWTQDTDMRVVLRINSAHYYAVVWVNGIHVV
  EHEGGHLPFEADISKLVQSGPLTTCRITIAINNTLTPHTLPPGTIVYKTDTSMYPKGYFVQDTSFDFFNY
  AGLHRSVVLYTTPTTYIDDITVITNVEQDIGLVTYWISVQGSEHFQLEVQLLDEGGKVVAHGTGNQGQLQ
  VPSANLWWPYLMHEHPAYMYSLEVKVTTTESVTDYYTLPIGIRTVAVTKSKFLINGKPFYFQGVNKHEDS
  DIRGKGFDWPLLVKDFNLLRWLGANSFRTSHYPYSEEVLQLCDRYGIVVIDECPGVGIVLPQSFGNESLR
  HHLEVMEELVRRDKNHPAVVMWSVANEPSSALKPAAYYFKTLITHTKALDLTRPVTFVSNAKYDADLGAP
  YVDVICVNSYFSWYHDYGHLEVIQPQLNSQFENWYKTHQKPIIQSEYGADAIPGIHEDPPRMFSEEYQKA
  VLENYHSVLDQKRKEYVVGELIWNFADFMTNQSPLRVIGNKKGIFTRQRQPKTSAFILRERYWRIANETG
  GHGSGPRTQCFGSRPFTF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010368
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010368.2
• RefSeq Size: 2456 bp
• RefSeq ORF: 1947 bp
• Locus ID: 110006
• UniProt ID: P12265
• Cytogenetics: 5 68.32 cM
• Gene Summary: Plays an important role in the degradation of dermatan and keratan sulfates.[UniProtKB/Swiss-Prot Function]