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Zfp677 (NM_172486) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209632
Zfp677 (tGFP-tagged) - Mouse RIKEN cDNA A830058L05 gene (A830058L05Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $791.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zfp677
Synonyms A830058L05Rik; Tce8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209632 representing NM_172486
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGATTCTCCTGGAAATATGTCTCAGGGTCTGTTGGCATTCAAAGATGTGGCTGTGGAATTTTCCC
AAGAGGAGTGGGGATGCCTTGACTCTGCTCAGAGGTCTTTGTACATCAATGTGATGTTGGAGAATTACAA
CAACCTGGTCTCTGTGGAGAACTATTGTATACGTGATACTGTTGATCAACATGTGAATACAGAAAAAGAG
TCTTGTCAGTATAATGAGCTTGGCAAAATTCTTCATGAACACTTCAAATGTGCACTTTATAAAACAAGTA
TAACTATAGAAACCTCTAAAAACTACAGATGCTGTAGTGACAGGGATGTGTCTAATGACTCACCAAACGC
AGATAGACACAGAAGCACGCACACTGGAGAAGAACCGTACGAATCTAAACACTGTGAGAAAACTTTCAGT
TTGCGTTCCAACATTAGTCAAGATCAGAGACTCTATACTGCAAAGAAAGAGCACAGACAAGAAGAATATG
ATGAGCATTTCAGCTCTACACACAGACTGATGCAACAAATAAACTACATTGGAGAAAAACCACACCAGTG
TGGGAAATGTGGAAAATGCTTCAGTACTTCCTCGAATCTCACTGTACACCAGAGAATTCATACTGGAGAG
AAACCCTACAAGTGCAACCTTTGTGACAAATCTTTTTCCCAGAGCACAACTCTTAAAACACATCAAAGAC
TTCATACTGGGGAGAAGCCTTACAAATGCAGAGAATGTGGAATGTCGTTTCCTTACTTGTCATCCCTTAA
AGTCCATCAGAATTTGCATCCTGGAGCGAAGCCATACAAATGTAAGGAATGTGACAAATCCTTTACTATA
AAGTCAACTCTTATAAAACATCAGAGAAATCATACTGGAGAGAAACCCTACACGTGCAATGTTTGTGACA
GATCCTTTAGACAATGTGCAAATCTTAAAACACACCAAAGACTTCATACAGGAGAAAAACCTTACAAGTG
CAAGGAATGTGGAAAGGCATTTACTCAGTCCTCATCCCTTAAAAAACATCTGAATTTGCATACTGGAAAG
AAACCTTACAAATGTGAGGAATGTGACAAATCTTTTACCGAGAAGTCAACACTTACAACGCATAAAAGAA
TTCATACTGGAAAGAAACCCTATAAGTGCAACATTTGTGGCAAATCCTTTAACAGTTGTACAAATCTTAA
AACACATCAAACACTTCATACTGGGGAGAAACCTTACAAGTGCAAGGAATGTGGAAAGGCATTTCCTTAC
ATGTCATCCCTTAAAATACATCAGAATTTGCATCCTGGAGAGAAGCCTTATAAATGTAAGGAATGTGACA
AATCCTTTACTCTGAAGTCAAGTCTTAGAACACATCAGATAATTCATACTGGAGAGAAACCCTACAAGTG
CAACATTTGTGGCAAATCCTTTAACCAATGTACAAATCTTAAAACACATCAAAGACTTCATACTGGGGAG
AAACCTTATAAATGCAAAGAATGTGGAAAGTCATTTCATTACTGGTCATCCCTTAAAAGCCATCAGAATT
TGCATAGTGGAGAGAAGCCTTATAAATGTAAGGAATGTGACAAATCCTTTACTGAGAAGTCAACTCTTAT
AAAACATCAGAGAATTCACACAGGAAAGAAGCTCTACAAGTGCAACATTTGTGACAAATCCTTCACCTGG
TGTGCATCCCTTAAAACACATAAAAAGTTCCATACTGGAGAGAAACCTTACAAATGCAGAAAATGTGGAA
AGTCTTTTCCTCAGTTATCAACCCTTAAAAGACATCAGAAAATTCATGAAGACAAGGGACATACTGGGGA
GAAGCTTTACAAGTGCAATGACTGTGACAGGTCCTATTCCTACCATTCGTCATTTAGAAGGCATCAGAAA
ATTCATTCTTCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209632 representing NM_172486
Red=Cloning site Green=Tags(s)

MTDSPGNMSQGLLAFKDVAVEFSQEEWGCLDSAQRSLYINVMLENYNNLVSVENYCIRDTVDQHVNTEKE
SCQYNELGKILHEHFKCALYKTSITIETSKNYRCCSDRDVSNDSPNADRHRSTHTGEEPYESKHCEKTFS
LRSNISQDQRLYTAKKEHRQEEYDEHFSSTHRLMQQINYIGEKPHQCGKCGKCFSTSSNLTVHQRIHTGE
KPYKCNLCDKSFSQSTTLKTHQRLHTGEKPYKCRECGMSFPYLSSLKVHQNLHPGAKPYKCKECDKSFTI
KSTLIKHQRNHTGEKPYTCNVCDRSFRQCANLKTHQRLHTGEKPYKCKECGKAFTQSSSLKKHLNLHTGK
KPYKCEECDKSFTEKSTLTTHKRIHTGKKPYKCNICGKSFNSCTNLKTHQTLHTGEKPYKCKECGKAFPY
MSSLKIHQNLHPGEKPYKCKECDKSFTLKSSLRTHQIIHTGEKPYKCNICGKSFNQCTNLKTHQRLHTGE
KPYKCKECGKSFHYWSSLKSHQNLHSGEKPYKCKECDKSFTEKSTLIKHQRIHTGKKLYKCNICDKSFTW
CASLKTHKKFHTGEKPYKCRKCGKSFPQLSTLKRHQKIHEDKGHTGEKLYKCNDCDRSYSYHSSFRRHQK
IHSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172486
ORF Size 1902 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172486.2, NP_766074.2
RefSeq Size 2916 bp
RefSeq ORF 1905 bp
Locus ID 210503
Cytogenetics 17 A3.2
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Documents
Resources
"NM_172486" in other vectors (4)
SKU Description Size Price
MC202382 Zfp677 (untagged) - Mouse zinc finger protein 677 (Zfp677), (10ug) 10 ug
$591.00
MR209632 Zfp677 (Myc-DDK-tagged) - Mouse zinc finger protein 677 (Zfp677) 10 ug
$289.00 $591.00
MR209632L3 Lenti ORF clone of Zfp677 (Myc-DDK-tagged) - Mouse zinc finger protein 677 (Zfp677) 10 ug
$891.00
MR209632L4 Lenti ORF clone of Zfp677 (mGFP-tagged) - Mouse zinc finger protein 677 (Zfp677) 10 ug
$891.00

Plasmid Map for MG209632

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209632
Circular map for MG209632

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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