Xrcc6 (NM_010247) Mouse Tagged ORF Clone

CAT#: MG209374

Xrcc6 (tGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 6 (Xrcc6)

Product Images

Circular map for MG209374

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Xrcc6
• Synonyms: 70kDa; G22p1; Ku70
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209374 representing NM_010247
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGTGGGAGTCCTACTACAAAACTGAGGGCGAGGAAGAGGAAGAGGAGGAGGAGAGCCCCGACA
  CAGGTGGAGAATATAAATATTCAGGAAGAGATAGCTTGATTTTTCTGGTTGATGCCTCCAGGGCTATGTT
  CGAATCTCAGGGTGAAGATGAACTCACACCTTTTGATATGAGCATCCAGTGTATCCAGAGTGTGTACACC
  AGTAAGATCATAAGCAGCGATCGGGATCTCCTGGCAGTGGTGTTCTATGGAACCGAGAAAGATAAAAATT
  CAGTGAATTTCAAAAATATTTATGTCTTACAAGATTTGGACAACCCAGGCGCTAAGCGAGTGCTAGAGCT
  CGACCAGTTTAAGGGACAACAGGGGAAGAAGCACTTCCGAGACACGGTTGGCCATGGGTCTGACTACTCT
  TTGAGTGAAGTGCTCTGGGTCTGTGCCAACCTCTTCAGCGACGTCCAGCTCAAGATGAGTCACAAGAGGA
  TCATGCTGTTCACCAATGAAGACGACCCCCATGGCCGTGACAGTGCTAAAGCCAGCCGGGCCAGGACCAA
  AGCCAGCGACCTCCGGGACACTGGGATCTTCCTTGACTTGATGCATCTGAAGAAGCCAGGAGGCTTTGAT
  GTATCCGTGTTCTACAGGGACATCATCACCACCGCTGAGGACGAGGACCTTGGGGTTCACTTCGAGGAGT
  CAAGCAAGCTGGAAGACCTGCTAAGGAAGGTTCGAGCCAAGGAGACCAAAAAGCGAGTTCTGTCCAGGTT
  AAAGTTTAAGCTCGGTGAAGACGTAGTACTCATGGTGGGCATTTATAACTTGGTCCAGAAAGCTAACAAG
  CCTTTTCCAGTGAGACTCTATCGGGAAACAAATGAACCAGTGAAAACCAAGACAAGGACTTTTAATGTAA
  ACACCGGCAGTCTACTCCTGCCTAGTGACACCAAGCGGTCTCTGACTTACGGGACACGTCAGATTGTGCT
  GGAGAAAGAGGAGACAGAGGAGCTGAAGCGGTTTGATGAGCCAGGTTTGATCCTCATGGGCTTTAAGCCC
  ACGGTGATGCTGAAGAAGCAGCACTACCTGAGGCCCTCTCTGTTCGTGTACCCAGAGGAGTCCCTGGTCA
  GTGGGAGCTCAACCTTGTTCAGCGCTCTGCTCACCAAGTGTGTGGAGAAGAAGGTCATAGCAGTGTGTAG
  ATACACACCCCGGAAGAACGTCTCCCCGTATTTTGTGGCTTTGGTGCCCCAGGAAGAGGAGCTGGATGAT
  CAGAACATTCAGGTGACTCCAGGAGGCTTCCAGCTTGTCTTCCTCCCTTATGCCGATGACAAGCGGAAGG
  TGCCCTTTACTGAGAAGGTGACGGCCAACCAGGAGCAGATAGACAAGATGAAGGCCATTGTTCAAAAGCT
  CCGCTTCACATACAGGAGCGACAGTTTTGAGAATCCAGTCCTGCAGCAGCACTTCCGCAACCTGGAGGCC
  CTAGCTTTGGACATGATGGAGTCGGAGCAAGTGGTAGATCTGACACTACCCAAGGTTGAAGCCATAAAGA
  AAAGACTGGGTTCCCTGGCAGATGAGTTTAAAGAACTTGTCTATCCTCCAGGTTATAATCCCGAGGGAAA
  AGTTGCCAAGAGAAAACAAGATGATGAAGGTTCTACGAGTAAAAAGCCCAAGGTAGAGTTATCAGAAGAA
  GAGCTGAAGGCCCATTTTCGTAAGGGCACACTGGGTAAGCTCACTGTACCTACACTGAAGGACATATGCA
  AGGCTCATGGGCTTAAGAGTGGGCCGAAGAAGCAGGAACTGCTAGATGCTCTTATCAGACACTTGGAGAA
  GAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209374 representing NM_010247
  Red=Cloning site Green=Tags(s)
  
  MSEWESYYKTEGEEEEEEEESPDTGGEYKYSGRDSLIFLVDASRAMFESQGEDELTPFDMSIQCIQSVYT
  SKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQDLDNPGAKRVLELDQFKGQQGKKHFRDTVGHGSDYS
  LSEVLWVCANLFSDVQLKMSHKRIMLFTNEDDPHGRDSAKASRARTKASDLRDTGIFLDLMHLKKPGGFD
  VSVFYRDIITTAEDEDLGVHFEESSKLEDLLRKVRAKETKKRVLSRLKFKLGEDVVLMVGIYNLVQKANK
  PFPVRLYRETNEPVKTKTRTFNVNTGSLLLPSDTKRSLTYGTRQIVLEKEETEELKRFDEPGLILMGFKP
  TVMLKKQHYLRPSLFVYPEESLVSGSSTLFSALLTKCVEKKVIAVCRYTPRKNVSPYFVALVPQEEELDD
  QNIQVTPGGFQLVFLPYADDKRKVPFTEKVTANQEQIDKMKAIVQKLRFTYRSDSFENPVLQQHFRNLEA
  LALDMMESEQVVDLTLPKVEAIKKRLGSLADEFKELVYPPGYNPEGKVAKRKQDDEGSTSKKPKVELSEE
  ELKAHFRKGTLGKLTVPTLKDICKAHGLKSGPKKQELLDALIRHLEKN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010247
• ORF Size: 1824 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010247.1, NP_034377.1
• RefSeq Size: 2113 bp
• RefSeq ORF: 1827 bp
• Locus ID: 14375
• UniProt ID: P23475
• Cytogenetics: 15 38.33 cM
• Gene Summary: Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (By similarity).[UniProtKB/Swiss-Prot Function]