Plk1 (NM_011121) Mouse Tagged ORF Clone

CAT#: MG209333

Plk1 (tGFP-tagged) - Mouse polo-like kinase 1 (Drosophila) (Plk1)

Product Images

Circular map for MG209333

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plk1
• Synonyms: P; Plk; STPK; STPK13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209333 representing NM_011121
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCAGCGGCCAAAGCTGGAAAGCTGGCTCGAGCACCAGCCGACCTCGGGAAAGGTGGGGTTCCGG
  GAGATGCAGTTCCCGGTGCCCCAGTGGCCGCCCCGCTGGCGAAAGAAATTCCGGAGGTCCTAGTGGACCC
  ACGCAGCCGGCGGCAGTATGTACGGGGGCGCTTTCTGGGTAAAGGAGGCTTCGCCAAATGCTTCGAGATC
  TCAGACGCAGACACAAAAGAGGTGTTCGCAGGCAAGATCGTGCCTAAGTCTTTGCTGCTCAAGCCCCACC
  AGAAGGAGAAGATGTCTATGGAGATCTCAATTCACCGCAGCCTAGCACACCAACACGTCGTAGGCTTCCA
  TGACTTTTTTGAGGACAGCGACTTTGTATTTGTAGTTTTGGAGCTCTGTCGCAGGAGGTCCCTCCTGGAG
  CTGCACAAGAGGAGGAAGGCACTGACCGAGCCTGAGGCCCGCTACTACCTGCGACAGATAGTCCTGGGCT
  GCCAGTACCTGCACCGCAATCAGGTCATTCACAGGGACCTCAAGCTGGGCAACCTCTTCCTGAACGAGGA
  TCTGGAGGTGAAAATAGGGGATTTTGGCTTGGCAACCAAAGTGGAATATGAAGGGGAACGAAAGAAGACC
  TTGTGTGGCACTCCTAACTACATAGCTCCTGAGGTGCTGAGCAAGAAGGGACACAGTTTTGAGGTGGATG
  TGTGGTCCATTGGGTGCATCATGTATACCTTGCTAGTGGGCAAGCCTCCCTTTGAGACCTCGTGCCTAAA
  AGAGACCTACCTCCGGATCAAGAAAAATGAATACAGTATTCCCAAGCACATCAACCCAGTGGCCGCCTCC
  CTCATCCAGAAGATGCTTCAGACAGACCCCACTGCCCGCCCCACCATTCACGAGTTGCTCAATGACGAGT
  TCTTCACTTCTGGCTACATCCCCGCCCGTCTCCCTATTACCTGCCTCACCATCCCACCAAGGTTTTCAAT
  CGCTCCCAGCAGCCTGGACCCCAGCAGCAGGAAACCTCTCAAAGTCCTCAATAAAGGTGTGGAGAACCCC
  CTGCCTGACCGTCCCCGGGAGAAAGAGGAACCGGTGGTCCGGGAGACAAATGAGGCCATTGAGTGCCACC
  TTAGTGACTTGCTACAGCAGCTGACCAGTGTCAACGCCTCCAAGCCCTCGGAGCGCGGGCTGGTGCGGCA
  AGAGGAGGCTGAGGATCCTGCCTGCATCCCCATCTTCTGGGTCAGCAAGTGGGTGGACTATTCGGACAAG
  TATGGCCTTGGGTATCAGCTGTGTGACAACAGTGTGGGGGTGCTTTTTAATGACTCAACACGCCTGATTC
  TCTACAATGACGGGGACAGCCTGCAGTACATAGAGCGTGATGGCACGGAGTCCTATCTCACTGTGAGCTC
  CCATCCCAATTCCTTGATGAAGAAGATCACTCTCCTCAACTATTTCCGCAATTACATGAGTGAGCACCTG
  CTGAAGGCAGGGGCCAACATCACACCCCGGGAAGGCGACGAGCTGGCCCGGCTGCCCTACCTACGAACGT
  GGTTCCGCACACGCAGCGCCATCATCCTGCACCTCAGCAACGGCACCGTGCAGATTAACTTCTTCCAGGA
  CCACACCAAACTTATCCTGTGCCCCCTGATGGCAGCGGTGACCTACATCAACGAGAAGAGGGACTTCCAA
  ACGTACCGCCTGAGCCTCCTGGAGGAGTATGGCTGCTGCAAGGAGCTGGCCAGCCGCCTCCGCTACGCCC
  GCACCATGGTAGACAAGCTGCTGAGCTCGCGCTCCGCCAGCAACCGCCTCAAGGCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209333 representing NM_011121
  Red=Cloning site Green=Tags(s)
  
  MNAAAKAGKLARAPADLGKGGVPGDAVPGAPVAAPLAKEIPEVLVDPRSRRQYVRGRFLGKGGFAKCFEI
  SDADTKEVFAGKIVPKSLLLKPHQKEKMSMEISIHRSLAHQHVVGFHDFFEDSDFVFVVLELCRRRSLLE
  LHKRRKALTEPEARYYLRQIVLGCQYLHRNQVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYEGERKKT
  LCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAAS
  LIQKMLQTDPTARPTIHELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSSRKPLKVLNKGVENP
  LPDRPREKEEPVVRETNEAIECHLSDLLQQLTSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDK
  YGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLNYFRNYMSEHL
  LKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGTVQINFFQDHTKLILCPLMAAVTYINEKRDFQ
  TYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011121
• ORF Size: 1809 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011121.4
• RefSeq Size: 2203 bp
• RefSeq ORF: 1812 bp
• Locus ID: 18817
• UniProt ID: Q07832
• Cytogenetics: 7 65.52 cM
• Gene Summary: The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and may play a role in DNA replication during S phase. This gene is expressed in all embryonic tissues, but restricted to thymus and ovaries in adult tissues. Homozygous knockout mice were embryonic lethal, suggesting that this gene is important for early embryonic development. This gene is thought to be a potential oncogene because it is overexpressed in a variety of tumors and tumor cell lines. Depletion of this protein in cancer cells has been shown to inhibit cell proliferation and suppress oncogenic transformation; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]