Ptpn11 (NM_011202) Mouse Tagged ORF Clone

CAT#: MG209281

Ptpn11 (tGFP-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 11 (Ptpn11)

Product Images

Circular map for MG209281

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ptpn11
• Synonyms: 2700084A17Rik; AW536184; PTP1D; PTP2C; SAP-2; SH-PTP2; SH-PTP3; SHP-2; Shp2; Syp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209281 representing NM_011202
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATCGCGGAGATGGTTTCACCCCAACATCACTGGTGTGGAGGCAGAGAATCTCCTGCTGACCAGAG
  GAGTCGATGGCAGTTTTTTAGCAAGGCCCAGTAAGAGTAACCCTGGAGACTTCACTCTGTCTGTTAGAAG
  AAATGGAGCTGTTACCCACATCAAGATTCAGAACACTGGGGACTACTATGACCTCTATGGTGGGGAGAAG
  TTTGCCACTTTGGCTGAACTGGTTCAGTATTACATGGAACACCATGGGCAGCTGAAAGAGAAGAATGGAG
  ATGTTATCGAGCTCAAGTACCCGCTGAACTGTGCAGACCCTACCTCTGAAAGGTGGTTCCATGGTCACTT
  GTCTGGAAAAGAAGCAGAGAAGCTGCTGACGGAGAAGGGCAAGCATGGCAGCTTCCTCGTTCGAGAGAGC
  CAGAGCCACCCCGGAGACTTCGTTCTCTCCGTGCGCACTGGTGACGACAAAGGGGAGAGCAACGACGGCA
  AGTCCAAAGTGACCCACGTCATGATCCGCTGTCAGGAGCTGAAATACGACGTTGGTGGGGGAGAGCGCTT
  TGACTCTCTGACAGACCTGGTGGAGCATTACAAGAAGAACCCCATGGTGGAGACGCTGGGCACAGTCCTG
  CAGCTCAAGCAGCCCCTCAACACAACTCGTATCAATGCTGCTGAAATTGAAAGCCGGGTTCGAGAGTTAA
  GCAAGCTGGCTGAGACCACAGATAAAGTCAAGCAGGGCTTTTGGGAAGAGTTTGAGACGCTCCAGCAACA
  GGAATGCAAACTTCTCTATAGCCGAAAAGAAGGACAGAGACAAGAAAATAAAAACAAAAACAGATACAAA
  AACATCCTGCCCTTTGATCATACCAGGGTCGTTCTGCATGATGGGGATCCCAATGAGCCTGTTTCTGATT
  ACATTAATGCAAACATCATCATGCCTGAGTTTGAGACCAAGTGCAACAATTCCAAACCCAAAAAGAGTTA
  CATTGCCACTCAAGGCTGCCTGCAGAACACGGTGAATGACTTCTGGCGGATGGTGTTCCAGGAGAACTCT
  CGAGTCATTGTCATGACCACAAAGGAAGTGGAGAGAGGGAAGAGCAAATGTGTCAAGTACTGGCCTGATG
  AGTATGCGCTCAAAGAATACGGGGTCATGCGTGTTAGGAACGTCAAAGAAAGTGCCGCCCATGACTACAC
  TTTACGAGAGCTCAAACTCTCTAAGGTCGGACAAGCTCTACTCCAGGGAAACACAGAGAGAACCGTCTGG
  CAGTACCACTTTCGGACCTGGCCAGACCATGGCGTGCCTAGTGACCCTGGAGGTGTGCTGGACTTCCTGG
  AGGAGGTCCACCACAAGCAGGAGAGCATCGTGGATGCAGGCCCTGTCGTGGTTCACTGCAGCGCTGGGAT
  TGGCCGGACAGGAACCTTCATTGTGATTGACATCCTTATTGACATCATTCGAGAGAAAGGTGTGGACTGT
  GACATCGACGTTCCTAAAACCATTCAGATGGTGCGGTCCCAGAGGTCGGGGATGGTCCAGACAGAAGCAC
  AGTACCGGTTTATCTACATGGCTGTCCAGCACTACATAGAGACGCTGCAGCGCCGGATCGAGGAGGAGCA
  GAAAAGCAAAAGAAAAGGACATGAATATACCAATATTAAGTATTCCTTGGTGGACCAGACAAGTGGTGAT
  CAGAGTCCCCTGCCACCCTGCACCCCAACGCCACCCTGTGCAGAAATGAGGGAGGACAGCGCCCGAGTCT
  ATGAGAACGTGGGCCTCATGCAGCAGCAGAGGAGTTTCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209281 representing NM_011202
  Red=Cloning site Green=Tags(s)
  
  MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEK
  FATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRES
  QSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVL
  QLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYK
  NILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENS
  RVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQALLQGNTERTVW
  QYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIVDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDC
  DIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLVDQTSGD
  QSPLPPCTPTPPCAEMREDSARVYENVGLMQQQRSFR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011202
• ORF Size: 1791 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011202.3, NP_035332.1
• RefSeq Size: 5628 bp
• RefSeq ORF: 1794 bp
• Locus ID: 19247
• UniProt ID: P35235
• Cytogenetics: 5 F
• Gene Summary: Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73.[UniProtKB/Swiss-Prot Function]