Ezr (NM_009510) Mouse Tagged ORF Clone

CAT#: MG209152

Ezr (tGFP-tagged) - Mouse villin 2 (Vil2)

Product Images

Circular map for MG209152

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ezr
• Synonyms: AW146364; p81; R75297; Vil2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209152 representing NM_009510
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAGCCAATCAACGTCCGGGTGACCACCATGGATGCCGAGCTGGAGTTTGCCATCCAGCCAAACA
  CCACCGGGAAGCAGCTCTTTGATCAGGTGGTAAAGACGATTGGCCTCCGGGAAGTGTGGTACTTCGGCCT
  CCAGTATGTAGACAATAAAGGATTTCCTACCTGGCTGAAACTTGATAAAAAGGTCTCCGCACAGGAGGTC
  CGAAAGGAGAACCCTGTCCAGTTTAAATTCCGGGCCAAGTTCTACCCCGAAGACGTGGCCGAGGAACTCA
  TCCAGGACATCACACAGAAGCTCTTCTTCCTGCAAGTCAAGGACGGGATCCTCAGCGACGAGATCTACTG
  CCCCCCAGAGACAGCCGTGCTCCTGGGCTCCTATGCCGTTCAGGCCAAGTTCGGAGATTATAACAAGGAA
  ATGCACAAGTCTGGGTACCTCAGCTCGGAGCGGCTGATCCCCCAGAGAGTCATGGACCAACACAAGCTCA
  GCAGGGACCAGTGGGAGGACCGGATCCAGGTGTGGCACGCGGAACACCGAGGGATGCTCAAGGACAGTGC
  TATGCTAGAATACCTGAAGATTGCCCAGGACCTGGAAATGTATGGGATCAACTATTTCGAGATCAAAAAC
  AAGAAAGGAACAGACCTTTGGCTTGGAGTCGATGCCCTTGGACTTAACATTTATGAGAAAGATGACAAGT
  TGACCCCAAAGATCGGCTTCCCTTGGAGTGAGATCAGGAACATCTCTTTCAACGACAAGAAGTTTGTCAT
  TAAGCCCATCGACAAGAAGGCACCTGACTTTGTGTTCTACGCCCCGCGCCTGAGAATTAACAAGCGGATC
  CTGCAGCTCTGCATGGGGAACCATGAGCTGTACATGCGCCGCAGGAAGCCCGACACCATCGAGGTGCAGC
  AGATGAAGGCCCAGGCTCGGGAGGAGAAGCACCAGAAGCAGCTAGAGCGACAGCAGTTGGAAACCGAGAA
  GAAGAGGCGAGAGACGGTGGAGAGAGAAAAGGAGCAGATGCTCCGGGAGAAGGAGGAGCTGATGCTTCGG
  CTGCAGGACTACGAGCAGAAGACCAAGAGGGCGGAGAAAGAGCTCTCCGAGCAGATTGAGAAGGCCCTCC
  AACTGGAGGAAGAGAGGAGGCGAGCCCAGGAGGAGGCTGAACGTCTGGAGGCCGACCGCATGGCCGCCCT
  GCGGGCCAAGGAAGAACTCGAGAGACAGGCGCAGGATCAGATAAAGAGCCAGGAGCAGCTGGCTGCAGAG
  CTGGCAGAGTACACGGCCAAGATCGCACTGCTGGAGGAGGCGCGGAGGCGCAAGGAGGACGAGGTAGAAG
  AGTGGCAGCACCGGGCTAAAGAAGCCCAGGACGACCTGGTGAAGACCAAAGAGGAGCTGCACCTGGTGAT
  GACGGCCCCACCGCCCCCACCGCCCCCAGTGTATGAGCCTGTGAATTACCACGTGCAGGAGGGACTGCAG
  GACGAGGGAGCAGAGCCTATGGGCTACAGTGCCGAGCTCTCCAGTGAGGGCATCCTGGATGACCGCAACG
  AGGAGAAGCGGATCACAGAGGCAGAGAAGAATGAGCGCGTGCAGCGGCAGCTGCTGACCCTGAGCAATGA
  GTTGTCCCAGGCCCGGGATGAGAACAAGAGGACCCACAATGACATCATCCACAACGAGAACATGCGGCAA
  GGCAGGGACAAGTATAAGACGCTGCGGCAAATCAGGCAGGGCAACACCAAGCAACGCATTGACGAGTTCG
  AGGCCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209152 representing NM_009510
  Red=Cloning site Green=Tags(s)
  
  MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLQYVDNKGFPTWLKLDKKVSAQEV
  RKENPVQFKFRAKFYPEDVAEELIQDITQKLFFLQVKDGILSDEIYCPPETAVLLGSYAVQAKFGDYNKE
  MHKSGYLSSERLIPQRVMDQHKLSRDQWEDRIQVWHAEHRGMLKDSAMLEYLKIAQDLEMYGINYFEIKN
  KKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRI
  LQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMLREKEELMLR
  LQDYEQKTKRAEKELSEQIEKALQLEEERRRAQEEAERLEADRMAALRAKEELERQAQDQIKSQEQLAAE
  LAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVNYHVQEGLQ
  DEGAEPMGYSAELSSEGILDDRNEEKRITEAEKNERVQRQLLTLSNELSQARDENKRTHNDIIHNENMRQ
  GRDKYKTLRQIRQGNTKQRIDEFEAM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009510
• ORF Size: 1758 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009510.2, NP_033536.2
• RefSeq Size: 3070 bp
• RefSeq ORF: 1761 bp
• Locus ID: 22350
• UniProt ID: P26040
• Cytogenetics: 17 A1
• Gene Summary: Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis (By similarity).[UniProtKB/Swiss-Prot Function]