Arx (NM_007492) Mouse Tagged ORF Clone

CAT#: MG208926

Arx (tGFP-tagged) - Mouse aristaless related homeobox gene (Drosophila) (Arx)

Product Images

Circular map for MG208926

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arx
• Synonyms: Ar; Arx1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208926 representing NM_007492
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAATCAGTACCAGGAAGAGGGCTGCTCTGAGAGGCCGGAGTGCAAGAGTAAATCTCCAACTTTGC
  TCTCCTCCTACTGCATCGACAGCATCCTGGGCCGGAGAAGCCCATGCAAAATGAGGCTTCTGGGAGCCGC
  GCAGAGCTTGCCTGCCCCGCTGGCCAGCCGCGCTGACCAGGAAAAAGCCATGCAAGGCTCCCCCAAGAGC
  AGCAGCGCCCCGTTCGAGGCTGAGCTGCACCTGCCACCCAAGCTGCGGCGCCTCTACGGCCCGGGCGGGG
  GCCGCCTCCTCCAGGGCGCGGCGGCGGCGGCTGCAGCGGCGGCGGCGGCCGCAGCGGCGGCCACGGCCAC
  TGGCACCGCAGGTCCCCGTGGAGAGGTTCCTCCACCGCCACCACCAGCTGCACGGCCAGGGGAGCGTCAG
  GACAGCGCAGGGGCCGTGGCTGCCGCCGCTGCCGCCGCTGCCTGGGACACGCTCAAGATCAGCCAGGCGC
  CGCAGGTGAGCATCAGTCGTAGCAAGTCGTACCGCGAGAACGGGGCGCCCTTCGTGCCCCCGCCGCCAGC
  CCTGGACGAGCTGAGTGGCCCGGGGGGCGTCGCGCACCCCGAGGAGCGCCTCAGCGCGGCCAGCGGACCC
  GGCAGTGCCCCCGCGGCGGGTGGTGGCACGGGCGCCGAGGACGACGAGGAGGAGCTGTTGGAGGATGAGG
  AAGATGAAGAGGAGGAAGAGGAGCTGCTGGAGGACGACGACGAGGAGCTGCTGGAGGACGATGCCCGCGC
  GCTGCTCAAGGAGCCCCGGCGCTGCTCGGTGGCCACCACCGGCACAGTGGCCGCCGCCGCCGCCGCTGCT
  GCCGCCGCCGTGGCCACCGAGGGCGGGGAGCTGTCGCCCAAGGAAGAGCTGTTGCTGCACCCGGAGGACG
  CCGAGGGCAAGGATGGTGAGGACAGCGTGTGCCTGTCGGCCGGCAGCGACTCGGAGGAGGGGCTGCTGAA
  GCGCAAACAGAGGCGCTACCGCACCACGTTCACCAGTTACCAGCTGGAGGAACTGGAGCGGGCTTTCCAG
  AAGACGCACTACCCTGACGTCTTCACCAGGGAGGAGCTGGCCATGAGGCTGGACCTGACAGAGGCCCGAG
  TCCAGGTGTGGTTCCAGAACCGTCGGGCCAAGTGGCGCAAGCGGGAGAAGGCTGGCGCACAGACCCACCC
  CCCGGGGCTGCCCTTTCCAGGGCCGCTCTCGGCTACCCACCCTCTCAGCCCCTACCTGGACGCCAGCCCC
  TTTCCCCCGCATCACCCTGCGCTTGATTCGGCCTGGACCGCCGCGGCGGCGGCGGCTGCAGCTGCCTTCC
  CGAGCCTACCTCCGCCCCCAGGCTCTGCTAGCCTGCCACCCAGCGGGGCGCCGCTGGGTCTGAGCACTTT
  TCTAGGAGCAGCGGTGTTCCGCCACCCAGCCTTCATCAGCCCAGCATTTGGCAGGCTCTTTTCCACTATG
  GCCCCCCTGACCAGCGCGTCGACTGCGGCTGCGCTCCTGAGACAGCCCACACCCGCGGTGGAGGGCGCAG
  TGGCCTCGGGCGCGCTGGCCGACCCGGCCACCGCCGCGGCAGACAGACGCGCGTCAAGCATAGCCGCGCT
  GAGGCTCAAGGCTAAGGAGCACGCCGCGCAGCTCACGCAGCTCAACATCCTGCCGGGCACCAGCACGGGG
  AAGGAGGTGTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208926 representing NM_007492
  Red=Cloning site Green=Tags(s)
  
  MSNQYQEEGCSERPECKSKSPTLLSSYCIDSILGRRSPCKMRLLGAAQSLPAPLASRADQEKAMQGSPKS
  SSAPFEAELHLPPKLRRLYGPGGGRLLQGAAAAAAAAAAAAAAATATGTAGPRGEVPPPPPPAARPGERQ
  DSAGAVAAAAAAAAWDTLKISQAPQVSISRSKSYRENGAPFVPPPPALDELSGPGGVAHPEERLSAASGP
  GSAPAAGGGTGAEDDEEELLEDEEDEEEEEELLEDDDEELLEDDARALLKEPRRCSVATTGTVAAAAAAA
  AAAVATEGGELSPKEELLLHPEDAEGKDGEDSVCLSAGSDSEEGLLKRKQRRYRTTFTSYQLEELERAFQ
  KTHYPDVFTREELAMRLDLTEARVQVWFQNRRAKWRKREKAGAQTHPPGLPFPGPLSATHPLSPYLDASP
  FPPHHPALDSAWTAAAAAAAAAFPSLPPPPGSASLPPSGAPLGLSTFLGAAVFRHPAFISPAFGRLFSTM
  APLTSASTAAALLRQPTPAVEGAVASGALADPATAAADRRASSIAALRLKAKEHAAQLTQLNILPGTSTG
  KEVC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007492
• ORF Size: 1692 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_007492.4, NP_031518.2
• RefSeq Size: 2759 bp
• RefSeq ORF: 1695 bp
• Locus ID: 11878
• UniProt ID: O35085
• Cytogenetics: X 41.05 cM
• Gene Summary: This gene encodes a transcription factor that plays an important role in the development of forebrain. Male mice lacking this gene have smaller brains, olfactory bulbs and testes, and die within half a day after birth. Mice lacking this gene specifically in ganglionic eminence-derived neurons, including cortical interneurons, develop seizures. Mutations in this gene have been demonstrated to cause mouse phenotypes resembling human X-linked lissencephaly and cognitive disability with epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]