Hnrnpl (NM_177301) Mouse Tagged ORF Clone

CAT#: MG208796

Hnrnpl (tGFP-tagged) - Mouse heterogeneous nuclear ribonucleoprotein L (Hnrpl)

Product Images

Circular map for MG208796

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hnrnpl
• Synonyms: C79783; D830027H13Rik; Hnrpl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208796 representing NM_177301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGGAGGCTGCTGCCCCGGGCGGAGAAGCGGCGTCGGCGGCTCGAGCAGAGGCAGCAGCCGGACG
  AGCAGCTGAGGCGGGCGGGAGCGATGGTGAAGATGGCGGCTGCGGGCGGCGGAGGTGGCGGTGGTCGCTA
  TTACGGCGGCGGCAACGAGGGAGGCCGAGCCCCTAAACGGCTGAAGACTGAAAACGCCGGCGACCAGCAC
  GGAGGCGGCGGCGGCGGAGGCTCCGGGGCGGCGGGCGGCGGCGGCGGGGAGAACTACGATGACCCCCACA
  AAACCCCTGCCTCCCCAGTTGTTCACATCAGGGGCCTGATTGACGGAGTAGTGGAAGCTGACCTTGTGGA
  AGCCCTGCAAGAATTTGGACCCATCAGCTATGTGGTGGTGATGCCAAAGAAGAGGCAGGCACTGGTAGAG
  TTTGAAGACGTGCTGGGGGCTTGTAATGCCGTAAACTACGCTGCAGACAACCAGATCTACATTGCCGGTC
  ACCCTGCATTTGTCAATTATTCTACCAGCCAGAAGATCTCTCGCCCGGGCGACTCTGATGACTCCCGGAG
  CGTAAACAGCGTGCTTCTGTTTACCATCCTAAACCCCATCTACTCCATAACCACGGATGTTCTTTACACC
  ATCTGTAACCCTTGTGGCCCTGTCCAGAGAATTGTCATTTTCCGGAAGAACGGAGTTCAGGCTATGGTGG
  AGTTTGATTCTGTGCAAAGTGCCCAGCGCGCCAAGGCCTCACTCAATGGGGCTGACATCTATTCCGGCTG
  TTGTACTCTGAAAATTGAGTACGCAAAGCCTACGCGTTTAAATGTATTCAAGAACGATCAAGATACTTGG
  GACTACACGAACCCCAACCTCAGTGGACAAGGTGACCCTGGCAGTAACCCCAACAAACGCCAGCGGCAGC
  CCCCTCTCCTGGGAGATCATCCCGCAGAATATGGAGGGCCCCATGGTGGGTACCACAGCCATTACCATGA
  TGAGGGCTACGGGCCCCCCCCACCTCACTACGAAGGGAGAAGGATGGGCCCACCTGTGGGGGGTCACCGC
  CGGGGCCCAAGTCGCTATGGCCCCCAGTATGGTCATCCCCCACCCCCTCCCCCACCACCCGACTATGGCC
  CCCACGCTGACAGCCCTGTGCTCATGGTCTATGGCTTGGATCAATCTAAGATGAACTGTGATCGAGTCTT
  CAATGTCTTCTGCTTGTATGGCAATGTGGAGAAGGTGAAATTTATGAAAAGCAAGCCGGGGGCCGCCATG
  GTGGAAATGGCTGATGGCTATGCTGTGGACCGAGCCATTACTCACCTCAACAACAACTTCATGTTTGGGC
  AGAAGATGAATGTCTGTGTCTCCAAGCAACCAGCCATTATGCCTGGTCAGTCATACGGGCTAGAAGATGG
  ATCCTGCAGCTATAAAGACTTCAGCGAGTCAAGGAACAATCGGTTCTCCACTCCAGAGCAGGCAGCTAAG
  AACCGCATCCAGCACCCTAGCAATGTACTGCACTTCTTCAATGCTCCCCTCGAGGTGACTGAGGAGAACT
  TCTTTGAGATCTGCGATGAACTGGGAGTGAAGCGGCCAACCTCTGTCAAAGTATTTTCAGGCAAAAGCGA
  GCGGAGCTCCTCTGGGCTGCTGGAGTGGGACTCGAAGAGCGATGCCCTGGAGACCTTGGGCTTCCTGAAC
  CATTACCAGATGAAAAACCCAAATGGCCCGTACCCATACACTCTGAAGTTGTGCTTCTCCACTGCACAGC
  ACGCCTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208796 representing NM_177301
  Red=Cloning site Green=Tags(s)
  
  MSRRLLPRAEKRRRRLEQRQQPDEQLRRAGAMVKMAAAGGGGGGGRYYGGGNEGGRAPKRLKTENAGDQH
  GGGGGGGSGAAGGGGGENYDDPHKTPASPVVHIRGLIDGVVEADLVEALQEFGPISYVVVMPKKRQALVE
  FEDVLGACNAVNYAADNQIYIAGHPAFVNYSTSQKISRPGDSDDSRSVNSVLLFTILNPIYSITTDVLYT
  ICNPCGPVQRIVIFRKNGVQAMVEFDSVQSAQRAKASLNGADIYSGCCTLKIEYAKPTRLNVFKNDQDTW
  DYTNPNLSGQGDPGSNPNKRQRQPPLLGDHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGRRMGPPVGGHR
  RGPSRYGPQYGHPPPPPPPPDYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAM
  VEMADGYAVDRAITHLNNNFMFGQKMNVCVSKQPAIMPGQSYGLEDGSCSYKDFSESRNNRFSTPEQAAK
  NRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPTSVKVFSGKSERSSSGLLEWDSKSDALETLGFLN
  HYQMKNPNGPYPYTLKLCFSTAQHAS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177301
• ORF Size: 1758 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177301.5, NP_796275.3
• RefSeq Size: 2142 bp
• RefSeq ORF: 1761 bp
• Locus ID: 15388
• UniProt ID: Q8R081
• Cytogenetics: 7 B1
• Gene Summary: Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion (PubMed:22523384). Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.[UniProtKB/Swiss-Prot Function]