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Rgs14 (NM_016758) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208709
Rgs14 (tGFP-tagged) - Mouse regulator of G-protein signaling 14 (Rgs14)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $710.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rgs14
Synonyms RPIP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208709 representing NM_016758
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGGGAAGCCCAAGCACTTGGGTGTCCCCAACGGGCGCATGGTTCTGGCTGTCTCAGATGGAGAAC
TGACCAGCACTGCAGGTTCCCAGGCCCAGGGTGAGGGCAGAGGCAGCTCTCTCAGCATCCACAGCCTCCC
TAGTGGCCCCAGCAGCCCCTTCTCCACCGAGGAGCAGCCTGTGGCCAGCTGGGCCCAGTCCTTTGAGCGG
CTGCTGCAAGACCCACGGGGTCTGGCTTACTTCACTGAGTTCCTGAAGAAGGAATTCAGCGCAGAGAACG
TAACTTTCTGGAAAGCCTGCGAACGTTTCCAGCAGATCCCAGCCAGCGACACCAAGCAGCTAGCTCAGGA
GGCCCACAACATCTACCACGAGTTCCTATCCAGCCAGGCGCTGAGCCCAGTGAACATCGACCGACAGGCC
TGGCTTAGTGAGGAGGTGCTGGCCCAGCCCCGGCCAGATATGTTCCGAGCACAGCAGCTTCAGATCTTCA
ATTTGATGAAGTTCGACAGCTATGCGCGCTTCGTCAAATCCCCGCTGTACCAAGAGTGCCTGCTGGCGGA
GGCCGAGGGACGCCCCCTGCGGGAACCTGGCTCCTCACACCTCGGGAGCCCGGACACAGCGAGGAAGAAG
CCAAAGCTGAAGCCTGGGAAGTCACTGCCGCTGGGCGTGGAAGAGTTGGGGCAGCTGCCACTCGCTGAGG
GCCCTTGTGGCCGCCCTCTCCGCAAGTCCTTTCGTAGAGAGATGACAGGTGGAGCCATGAATTCGGCCCT
GCGACGAGAGTCTCAAGGGTCCCTGAATTCTTCTGCCAGTCTGGACCTGGGTTTCCTTGCCTTTGTGAGC
AGCAAATCTGAGAGTCACCGGAAGAGCCTTGGAAGTGGAGAGAGTGAGAGCGAGAGTCGGCCGGGGAAGT
ATTGCTGCGTGTATCTACCTGACGGCACGGCTTCCTTGGCCCTGGCTCGACCTGGCCTCACCATCCGAGA
CATGCTGGCAGGCATCTGTGAGAAGAGAGGCCTCTCTCTGCCTGACATTAAGGTCTACCTGGTGGGCAAT
GAACAGAAGGCCCTGGTCCTGGATCAGGATTGCACCGTGCTGGCAGACCAGGAAGTGCGACTGGAAAACA
GGATCACCTTCCAGCTGGAGTTGGTCGGCCTGGAGCGAGTGGTCCGGATCTCAGCTAAGCCCACCAAGCG
TCTGCAAGAGGCGCTGCAGCCCATCCTGGCTAAGCATGGCCTGAGCCTGGACCAGGTGGTACTGCACAGG
CCAGGAGAGAAGCAGCCCATGGATTTGGAGAATCCAGTGAGCTCAGTGGCCTCACAGACACTGGTTTTGG
ACACTCCTCCGGATGCAAAGATGAGTGAAGCCAGAAGCATATCCCCCTGCCGCAGTCAGGGATGCCTCCC
AAGAACCCAGACCAAGGACAGTCACCTTCCCCCATCGTCCTCCAGTTTGCTGGTAGAAGATGCCAGTAGT
TCTACTGGGAACCGGCAGACCTGTGACATTGAAGGCCTGGTGGAGCTGCTGAATCGGGTGCAGAGCAGCG
GGGCCCACGATCAGAGAGGACTTCTTCGCAAAGAGGACCTGGTCCTTCCAGAATTTCTGCAGCTTCCTTC
CCAAAGACCAGGCTCTCGGGAGGCTCCACCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208709 representing NM_016758
Red=Cloning site Green=Tags(s)

MPGKPKHLGVPNGRMVLAVSDGELTSTAGSQAQGEGRGSSLSIHSLPSGPSSPFSTEEQPVASWAQSFER
LLQDPRGLAYFTEFLKKEFSAENVTFWKACERFQQIPASDTKQLAQEAHNIYHEFLSSQALSPVNIDRQA
WLSEEVLAQPRPDMFRAQQLQIFNLMKFDSYARFVKSPLYQECLLAEAEGRPLREPGSSHLGSPDTARKK
PKLKPGKSLPLGVEELGQLPLAEGPCGRPLRKSFRREMTGGAMNSALRRESQGSLNSSASLDLGFLAFVS
SKSESHRKSLGSGESESESRPGKYCCVYLPDGTASLALARPGLTIRDMLAGICEKRGLSLPDIKVYLVGN
EQKALVLDQDCTVLADQEVRLENRITFQLELVGLERVVRISAKPTKRLQEALQPILAKHGLSLDQVVLHR
PGEKQPMDLENPVSSVASQTLVLDTPPDAKMSEARSISPCRSQGCLPRTQTKDSHLPPSSSSLLVEDASS
STGNRQTCDIEGLVELLNRVQSSGAHDQRGLLRKEDLVLPEFLQLPSQRPGSREAPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016758
ORF Size 1641 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016758.3
RefSeq Size 2437 bp
RefSeq ORF 1644 bp
Locus ID 51791
UniProt ID P97492
Cytogenetics 13 29.8 cM
Summary Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Besides, modulates signal transduction via G protein alpha subunits by functioning as a GDP-dissociation inhibitor (GDI). Has GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not on GNAI2 and G(o) alpha subunit GNAO1. Has GAP activity on GNAI0, GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division; required for completion of the first mitotic division of the embryo. Involved in visual memory processing capacity; when overexpressed in the V2 secondary visual cortex area. Involved in hippocampal-based learning and memory; acts as a suppressor of synaptic plasticity in CA2 neurons. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance.UniProtKB/Swiss-Prot Function
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"NM_016758" in other vectors (4)
SKU Description Size Price
MC204443 Rgs14 (untagged) - Mouse regulator of G-protein signaling 14 (Rgs14), (10ug) 10 ug
$510.00
MR208709 Rgs14 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 14 (Rgs14) 10 ug
$289.00 $510.00
MR208709L3 Lenti ORF clone of Rgs14 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 14 (Rgs14) 10 ug
$810.00
MR208709L4 Lenti ORF clone of Rgs14 (mGFP-tagged) - Mouse regulator of G-protein signaling 14 (Rgs14) 10 ug
$810.00

Plasmid Map for MG208709

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208709
Circular map for MG208709

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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