Gns (NM_029364) Mouse Tagged ORF Clone

CAT#: MG208675

Gns (tGFP-tagged) - Mouse glucosamine (N-acetyl)-6-sulfatase (Gns)

Product Images

Circular map for MG208675

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gns
• Synonyms: 2610016K11Rik; AU042285; C87209; G6S; N28088
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208675 representing NM_029364
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTCCCTTCGGCAGCCGGGCCCCGGCCGGGCCGTCCCCGCCGCCTGCCGGCGCTGCTGCTGCTGC
  CGCTGCTGGGAGGCTGCCTGGGGCTCGTGGGGGCGGCTCGCAGGCCCAACGTGCTGCTGCTGCTCACCGA
  CGACCAGGACGCGGAGCTCGGCGGCATGACGCCACTGAAGAAAACCAAGGCCCTCATCGGAGAGAAGGGG
  ATGACTTTCTCTAGCGCCTATGTGCCCAGTGCTCTTTGCTGCCCCAGCAGAGCCAGCATCCTGACTGGAA
  AGTACCCCCACAACCACCACGTCGTTAACAACACTTTGGAAGGGAACTGCAGTAGCAAGGCCTGGCAGAA
  GATCCAGGAGCCGTACACCTTCCCGGCCATCCTCAAGTCGGTGTGCGGCTATCAGACCTTTTTTGCTGGG
  AAGTATTTAAACGAGTATGGAGCCCCAGACGCAGGCGGACTGGAGCACATCCCCCTGGGCTGGAGTTACT
  GGTATGCCCTGGAAAAGAATTCTAAATACTATAACTACACCCTCTCCATCAACGGGAAGGCACGGAAGCA
  CGGCGAGAACTACAGCGTGGACTACCTGACAGATGTTCTGGCCAACCTCTCCCTGGACTTCCTGGACTAC
  AAGTCCAACTCTGAGCCATTCTTCATGATGATCTCCACTCCAGCGCCCCACTCACCGTGGACGGCTGCTC
  CGCAGTACCAGAAGGCTTTCCAGAATGTCATCGCACCAAGAAACAAGAACTTCAACATCCACGGGACGAA
  CAAGCACTGGCTAATCAGGCAAGCCAAGACTCCCATGACGAACTCGTCAATACGGTTCTTAGATGACGCC
  TTCAGGAGGAGGTGGCAAACCCTGCTCTCAGTTGACGACCTTGTGGAGAAACTGGTGAAGAGACTGGACT
  CGACCGGAGAGCTGGACAACACGTATATCTTCTATACCTCAGACAACGGCTACCACACAGGGCAGTTCTC
  TTTGCCAATAGACAAACGGCAGCTGTATGAGTTTGATATCAAAGTTCCACTGTTGGTTCGAGGGCCCGGC
  ATCAAACCAAACCAGACCAGCAAGATGCTGGTCTCAAACATCGACTTGGGTCCCACCATCCTGGACCTCG
  CTGGCTATGACCTGAACAAGACACAGATGGATGGGATGTCCCTGCTGCCCATTCTGAAAGGAGACAGAAA
  CTTAACCTGGAGGTCAGACGTGCTGGTGGAATATCAAGGAGAAGGCCGGAATGTCACCGACCCGACCTGC
  CCTTCTTTGAGTCCTGGAGTGTCTCAATGTTTCCCGGACTGTGTGTGTGAAGACGCGTACAACAATACCT
  ACGCCTGCGTGCGGACACTGTCATCATTGTGGAACCTCCAGTACTGCGAGTTCGACGACCAGGAGGTATT
  TGTCGAAGTCTACAATATCACTGCAGATCCAGACCAGATCACCAACATTGCCAAGAGTATAGACCCTGAG
  CTTTTGGGAAAGATGAACTATCGTCTGATGATGCTGCAGTCCTGTTCTGGGCCGACCTGTCGCACTCCAG
  GGGTCTTTGACCCTGGGTACAGGTTTGACCTGCGTCTCATGTTCAACAGTCACGGCAGCGTCAGAACGAG
  AAGGTTCTCGAAGCATCCGCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208675 representing NM_029364
  Red=Cloning site Green=Tags(s)
  
  MRLPSAAGPRPGRPRRLPALLLLPLLGGCLGLVGAARRPNVLLLLTDDQDAELGGMTPLKKTKALIGEKG
  MTFSSAYVPSALCCPSRASILTGKYPHNHHVVNNTLEGNCSSKAWQKIQEPYTFPAILKSVCGYQTFFAG
  KYLNEYGAPDAGGLEHIPLGWSYWYALEKNSKYYNYTLSINGKARKHGENYSVDYLTDVLANLSLDFLDY
  KSNSEPFFMMISTPAPHSPWTAAPQYQKAFQNVIAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIRFLDDA
  FRRRWQTLLSVDDLVEKLVKRLDSTGELDNTYIFYTSDNGYHTGQFSLPIDKRQLYEFDIKVPLLVRGPG
  IKPNQTSKMLVSNIDLGPTILDLAGYDLNKTQMDGMSLLPILKGDRNLTWRSDVLVEYQGEGRNVTDPTC
  PSLSPGVSQCFPDCVCEDAYNNTYACVRTLSSLWNLQYCEFDDQEVFVEVYNITADPDQITNIAKSIDPE
  LLGKMNYRLMMLQSCSGPTCRTPGVFDPGYRFDLRLMFNSHGSVRTRRFSKHPL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029364
• ORF Size: 1632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029364.4
• RefSeq Size: 3855 bp
• RefSeq ORF: 1635 bp
• Locus ID: 75612
• UniProt ID: Q8BFR4
• Cytogenetics: 10 D2