Wrap53 (NM_144824) Mouse Tagged ORF Clone

CAT#: MG208531

Wrap53 (tGFP-tagged) - Mouse WD repeat domain 79 (Wdr79)

Product Images

Circular map for MG208531

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wrap53
• Synonyms: BC021790; Wdr79
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208531 representing NM_144824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACGTCGGAGGAGCGACTCTTAGCTCCGGATAGCCTCCCTCCTGACCTGGCCCCAGCTCCGGTAC
  CCCAAGGTTCCCCAGCGGAGAAAAATACAGACTTTGAACCGGTGCCTCCGCCATGCGGTGGTGATGACCA
  ACCCCAGCTAGCCACAGATCCCGTGGCTAGCTTGGTTGTGTCCCAGGAGCTTCAACAAGGGGACTCTGTA
  CCTCTGGAAGTAGAATTTAATACTTCCAGTGAGTTGAGTCCTGGAATCGAGGAGCAAGATGTTTCTGAAC
  ATGCGAGTCTTCCCGGAGAAGAAACGAATCTCCCCGAGTTGGAGTCAGGAGAAGCCACGGAAGGTGTGTC
  TGAGGAACGCGCTGAAGTGGATGAGGGGGACACCTTTTGGACCTACAGCTTCTCGCAGGTACCCCGATAC
  CTCAGTGGTTCCTGGTCCGAGTTCAGCACCCGCTCCGAGAACTTCTTGAAAGGTTGCAAGTGGGCCCCTG
  ACGGTTCCTGTATCTTGACCAATAGTGCTGATAATGTCCTTCGGATTTACAACCTGCCCCCAGAATTGTA
  CAGTGAACAAGAGCAGGTGGATTACGCCGAAATGGTTCCTGTCCTTCGAATGGTGGAGGGCGACACCATC
  TATGATTACTGCTGGTATTCTCTGATGTCCTCCACCCAGCCAGACACCTCCTATGTGGCCAGCAGCAGCC
  GGGAGAACCCGATTCACATCTGGGATGCATTCACTGGAGAACTACGGGCTTCCTTTCGCGCTTACAACCA
  CCTGGATGAGTTGACAGCAGCCCACTCGCTCTGTTTCTCCCCGGATGGTTCCCAACTCTTCTGTGGCTTC
  AACCGGACTGTGCGTGTCTTTTCCACATCCAGGCCTGGCAGAGACTGTGAGGTGCGAGCCACATTTGCCA
  AAAAGCAGGGCCAGAGCGGTATCATCTCCTGTATAGCCTTCAGTCCATCCCAGCCCCTCTATGCCTGTGG
  CTCCTACGGCCGCACCATAGGCCTGTATGCCTGGGATGATGGTTCGCCTCTTGCCTTGCTGGGAGGCCAC
  CAAGGGGGTATCACCCACCTTTGCTTTCACCCTGATGGCAATCTCTTCTTCTCGGGAGCCCGAAAGGATG
  CTGAACTTCTGTGCTGGGATCTCCGGCAGCCAGGCCACCTCCTGTGGTCTCTGAGTCGGGAAGTGACCAC
  TAATCAGCGCATCTACTTTGATCTGGACCCGAGCGGTCAGTTCCTAGTGAGCGGCAACACCAGTGGAGTT
  GTCTCTGTGTGGGATATCAGTGGAGCTCTTAGTGACGACAGCAAGCTGGAACCCGTGGTGACTTTTCTGC
  CTCAGAAGGACTGCACCAACGGAGTCAGCCTTCACCCTACCTTGCCTCTACTGGCCACTGCTTCTGGCCA
  GCGCGTGTTCCCTGAGCCCACCAATAGCGGGGATGAAGGTGAGCTGGAGCTGGAGCTTCCTCTGCTCTCC
  CTGTGCCACGCCCATCCCGAATGTCAGCTTCAGCTCTGGTGGTGTGGAGGGGGACCTGACCCCAGTAGCC
  CTGTTGACGACCAGGATGAAAAGGGACAAAGAAGGACAGAGGCTGTGGGGATGAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208531 representing NM_144824
  Red=Cloning site Green=Tags(s)
  
  MKTSEERLLAPDSLPPDLAPAPVPQGSPAEKNTDFEPVPPPCGGDDQPQLATDPVASLVVSQELQQGDSV
  PLEVEFNTSSELSPGIEEQDVSEHASLPGEETNLPELESGEATEGVSEERAEVDEGDTFWTYSFSQVPRY
  LSGSWSEFSTRSENFLKGCKWAPDGSCILTNSADNVLRIYNLPPELYSEQEQVDYAEMVPVLRMVEGDTI
  YDYCWYSLMSSTQPDTSYVASSSRENPIHIWDAFTGELRASFRAYNHLDELTAAHSLCFSPDGSQLFCGF
  NRTVRVFSTSRPGRDCEVRATFAKKQGQSGIISCIAFSPSQPLYACGSYGRTIGLYAWDDGSPLALLGGH
  QGGITHLCFHPDGNLFFSGARKDAELLCWDLRQPGHLLWSLSREVTTNQRIYFDLDPSGQFLVSGNTSGV
  VSVWDISGALSDDSKLEPVVTFLPQKDCTNGVSLHPTLPLLATASGQRVFPEPTNSGDEGELELELPLLS
  LCHAHPECQLQLWWCGGGPDPSSPVDDQDEKGQRRTEAVGMS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144824
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144824.1
• RefSeq Size: 1896 bp
• RefSeq ORF: 1599 bp
• Locus ID: 216853
• UniProt ID: Q8VC51
• Cytogenetics: 11 B3
• Gene Summary: RNA chaperone that plays a key role in telomere maintenance and RNA localization to Cajal bodies (PubMed:29804836). Specifically recognizes and binds the Cajal body box (CAB box) present in both small Cajal body RNAs (scaRNAs) and telomerase RNA template component (TERC) (PubMed:29804836). Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes (By similarity). In the telomerase holoenzyme complex, required to stimulate the catalytic activity of the complex (PubMed:29804836). Acts by specifically binding the CAB box of the TERC RNA and controlling the folding of the CR4/CR5 region of the TERC RNA, a critical step for telomerase activity (By similarity). In addition, also controls telomerase holoenzyme complex localization to Cajal body (By similarity). During S phase, required for delivery of TERC to telomeres during S phase and for telomerase activity (By similarity). In addition to its role in telomere maintenance, also required for Cajal body formation, probably by mediating localization of scaRNAs to Cajal bodies (By similarity). Also plays a role in DNA repair: phosphorylated by ATM in response to DNA damage and relocalizes to sites of DNA double-strand breaks to promote the repair of DNA double-strand breaks (By similarity). Acts by recruiting the ubiquitin ligase RNF8 to DNA breaks and promote both homologous recombination (HR) and non-homologous end joining (NHEJ) (By similarity).[UniProtKB/Swiss-Prot Function]