Btn1a1 (NM_013483) Mouse Tagged ORF Clone

CAT#: MG208410

Btn1a1 (tGFP-tagged) - Mouse butyrophilin, subfamily 1, member A1 (Btn1a1)

Product Images

Circular map for MG208410

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Btn1a1
• Synonyms: Btn
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208410 representing NM_013483
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTTCCCACCAACTCCTGCCTCCTGGTCTGTCTGCTCACCCTCACTGTCCTACAGCTGCCCACGC
  TGGATTCGGCTCCCTTCGATGTGACCGCACCTCAGGAGCCAGTGTTGGCCCTAGTGGGCTCAGATGCCGA
  GCTGACCTGTGGCTTTTCCCCAAACGCGAGCTCAGAATACATGGAGCTGCTGTGGTTTCGACAGACGAGG
  TCGACAGCGGTACTTCTATACCGGGATGGCCAGGAGCAGGAGGGCCAGCAGATGACGGAGTACCGCGGGA
  GGGCGACGCTGGCGACAGCCGGGCTTCTAGACGGCCGCGCTACTCTGCTGATCCGAGATGTCAGGGTCTC
  AGACCAGGGGGAGTACCGGTGCCTTTTCAAAGACAACGACGACTTCGAGGAGGCCGCCGTATACCTCAAA
  GTGGCTGCTGTGGGTTCAGATCCTCAAATCAGTATGACGGTTCAAGAGAATGGAGAAATGGAGCTGGAGT
  GCACCTCCTCTGGATGGTACCCAGAGCCTCAGGTGCAGTGGAGAACAGGCAACAGAGAGATGCTACCATC
  CACGTCAGAGTCCAAGAAGCATAATGAGGAAGGCCTGTTCACTGTGGCAGTTTCAATGATGATCAGAGAC
  AGCTCCATAAAGAACATGTCCTGCTGCATCCAGAATATCCTCCTTGGCCAGGGGAAGGAAGTAGAGATCT
  CCTTACCAGCTCCCTTCGTGCCAAGGCTGACTCCCTGGATAGTAGCTGTGGCTATCATCTTACTGGCCTT
  AGGATTTCTCACCATTGGGTCCATATTTTTCACTTGGAAACTATACAAGGAAAGATCCAGTCTGCGGAAG
  AAGGAATTTGGCTCTAAAGAGAGACTTCTGGAAGAACTCAGATGCAAAAAGACTGTACTGCATGAAGTTG
  ACGTGACTCTGGATCCAGACACAGCCCACCCCCACCTCTTCCTGTATGAAGATTCAAAGTCAGTTCGATT
  GGAAGATTCACGTCAGATCCTGCCTGATAGACCAGAGAGATTTGACTCCTGGCCCTGTGTGTTGGGCCGT
  GAGACCTTTACTTCAGGGAGACATTACTGGGAGGTGGAGGTGGGAGATAGAACTGACTGGGCCATTGGTG
  TGTGTAGGGAGAATGTGGTGAAGAAAGGGTTTGACCCCATGACTCCTGATAATGGGTTCTGGGCTGTGGA
  GTTGTATGGAAATGGGTACTGGGCCCTCACCCCACTCAGGACCTCTCTCCGATTAGCAGGGCCCCCTCGC
  AGAGTTGGGGTTTTTCTGGACTATGACGCAGGAGACATTTCCTTCTACAACATGAGTAACGGATCTCTTA
  TCTATACTTTCCCTAGCATCTCTTTCTCTGGCCCCCTCCGTCCCTTCTTTTGTCTGTGGTCCTGTGGTAA
  AAAGCCCCTGACCATCTGTTCAACTGCCAATGGGCCTGAGAAAGTCACAGTCATTGCTAATGTCCAGGAC
  GACATTCCCTTGTCCCCGCTGGGGGAAGGCTGTACTTCTGGAGACAAAGACACTCTCCATTCTAAACTGA
  TCCCGTTCTCACCTAGCCAAGCGGCACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208410 representing NM_013483
  Red=Cloning site Green=Tags(s)
  
  MAVPTNSCLLVCLLTLTVLQLPTLDSAPFDVTAPQEPVLALVGSDAELTCGFSPNASSEYMELLWFRQTR
  STAVLLYRDGQEQEGQQMTEYRGRATLATAGLLDGRATLLIRDVRVSDQGEYRCLFKDNDDFEEAAVYLK
  VAAVGSDPQISMTVQENGEMELECTSSGWYPEPQVQWRTGNREMLPSTSESKKHNEEGLFTVAVSMMIRD
  SSIKNMSCCIQNILLGQGKEVEISLPAPFVPRLTPWIVAVAIILLALGFLTIGSIFFTWKLYKERSSLRK
  KEFGSKERLLEELRCKKTVLHEVDVTLDPDTAHPHLFLYEDSKSVRLEDSRQILPDRPERFDSWPCVLGR
  ETFTSGRHYWEVEVGDRTDWAIGVCRENVVKKGFDPMTPDNGFWAVELYGNGYWALTPLRTSLRLAGPPR
  RVGVFLDYDAGDISFYNMSNGSLIYTFPSISFSGPLRPFFCLWSCGKKPLTICSTANGPEKVTVIANVQD
  DIPLSPLGEGCTSGDKDTLHSKLIPFSPSQAAP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013483
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013483.2, NP_038511.1
• RefSeq Size: 4003 bp
• RefSeq ORF: 1575 bp
• Locus ID: 12231
• UniProt ID: Q62556
• Cytogenetics: 13 9.72 cM
• Gene Summary: May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.[UniProtKB/Swiss-Prot Function]