Cyp4a10 (NM_010011) Mouse Tagged ORF Clone

CAT#: MG208179

Cyp4a10 (tGFP-tagged) - Mouse cytochrome P450, family 4, subfamily a, polypeptide 10 (Cyp4a10)

Product Images

Circular map for MG208179

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyp4a10
• Synonyms: AI647584; Cyp4a; D4Rp1; Msp-3; RP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208179 representing NM_010011
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTCTCTGCTCTAAGCCCAACCCGATTTGCAGACAGCCTCTCTGGCTTCCTCCAAGTGGCCTCTG
  TGCTCGGTCTGCTCCTGCTGCTGGTGAAAGCTGTCCAGTTCTACCTCCACAGGCAATGGCTACTCAAGGC
  TTTCCAGCAGTTCCCATCACCTCCTTTTCACTGGTTCTTTGGGCATGAGCAGTTCAAAGGTGACCACGAA
  CTACAGGAAATTGTGTCGTGCATAGAGAACTTCCCAAGTGCCTTTCCTAGATGGTTCTGGGGAAGCAAGG
  CCTACTTAACTGTCTATGACCCTGACTACATGAAAGTGATTCTGGGGCGATCAGATCCAAAGGCTAATGG
  TGCGTACAGATTGCTAGCTCCTTGGATTGGGTATGGTTTGCTCTTGTTGAATGGACAGCCATGGTTCCAG
  CACAGGAGGATGCTAACCCCAGCCTTCCACTATGACATTCTGAAACCCTATGTGAAAAACATGGCCGACT
  CCATTCGATTGATGCTAGACAAATGGGAACGGCTGGCTGATCAGGACTCCTCTATAGAGATCTTTCAACA
  CATCTCCTTAATGACCCTAGACACTGTCATGAAATGTGCCTTCAGCCACAAGGGCAGTGTTCAGGTAGAT
  GGAAATTACAGGACCTACCTCCAGGCCATTGGGGACTTGAATAACCTATTTCACTCCCGTGTGAGGAATA
  TCTTTCATCAGAATGATACCATCTACAAACTTTCTTCCAATGGCCGCTTGGCCAAACAAGCTTGTCAACT
  TGCCCATGATCACACAGATGGAGTGATCAAGCTGAGAAAGGATCAGCTGCAGGATGAGGGAGAGCTGGAA
  AAGATCAAGAAGAAGAGACGTTTGGATTTTCTGGATATCCTTTTATTTGCCAGAATGGAGAATGGGGACA
  GCATGTCTGACAAGGACCTACGTGCTGAGGTGGACACATTCATGTTCGAGGGCCATGACACCACAGCCAG
  TGGAGTCTCCTGGATCTTCTATGCTCTGGCCACACACCCTGATCACCAACAGAGATGCAGAGAGGAAGTT
  CAGAGCCTCCTGGGGGATGGATCCTCTATCACCTGGGATCACCTAGACCAGATTCCCTATACCACAATGT
  GCATCAAGGAGGCCCTAAGGCTCTACCCACCTGTCCCAGGCATTGTCAGAGAACTCAGTACATCTGTCAC
  CTTCCCTGATGGACGCTCTTTACCCAAAGGTGTCCAGGTCACACTCTCCATTTATGGTCTCCACCACAAC
  CCGAAGGTGTGGCCAAATCCAGAGGTGTTTGACCCTTCCAGGTTTGCACCAGACTCTCCCCGACACAGCC
  ACTCATTCCTGCCCTTCTCAGGAGGAGCCAGGAACTGCATTGGGAAACAATTTGCTATGAGTGAGCTGAA
  GGTGATTGTGGCCCTGACACTGCTCCGCTTCGAACTACTGCCAGATCCTACCAGGGTCCCCATGCCCTTA
  GCCCGACTTGTGCTGAAGTCCAAAAATGGGATCTACCTACATCTCAAGAAGCTCCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208179 representing NM_010011
  Red=Cloning site Green=Tags(s)
  
  MSVSALSPTRFADSLSGFLQVASVLGLLLLLVKAVQFYLHRQWLLKAFQQFPSPPFHWFFGHEQFKGDHE
  LQEIVSCIENFPSAFPRWFWGSKAYLTVYDPDYMKVILGRSDPKANGAYRLLAPWIGYGLLLLNGQPWFQ
  HRRMLTPAFHYDILKPYVKNMADSIRLMLDKWERLADQDSSIEIFQHISLMTLDTVMKCAFSHKGSVQVD
  GNYRTYLQAIGDLNNLFHSRVRNIFHQNDTIYKLSSNGRLAKQACQLAHDHTDGVIKLRKDQLQDEGELE
  KIKKKRRLDFLDILLFARMENGDSMSDKDLRAEVDTFMFEGHDTTASGVSWIFYALATHPDHQQRCREEV
  QSLLGDGSSITWDHLDQIPYTTMCIKEALRLYPPVPGIVRELSTSVTFPDGRSLPKGVQVTLSIYGLHHN
  PKVWPNPEVFDPSRFAPDSPRHSHSFLPFSGGARNCIGKQFAMSELKVIVALTLLRFELLPDPTRVPMPL
  ARLVLKSKNGIYLHLKKLH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010011
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010011.2, NP_034141.2
• RefSeq Size: 2085 bp
• RefSeq ORF: 1530 bp
• Locus ID: 13117
• UniProt ID: O88833
• Cytogenetics: 4 53.05 cM
• Gene Summary: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.[UniProtKB/Swiss-Prot Function]