Tab1 (NM_025609) Mouse Tagged ORF Clone

CAT#: MG208071

Tab1 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 7 interacting protein 1 (Map3k7ip1)

Product Images

Circular map for MG208071

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tab1
• Synonyms: 2310012M03Rik; b2b449Clo; Map3k7ip1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208071 representing NM_025609
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCAGAGGAGGAGCCTGCTCCAGAGTGAGCAGCAGCCAAGCTGGACAGATGACCTGCCACTCT
  GTCACCTCTCTGGAGTTGGTTCAGCGTCCAACCGCAGCTACTCTGCTGATGGCAAGGGCACTGAGAGCCA
  CCCTCCGGAGGACAACTGGCTTAAGTTCAGAAGTGAAAATAACTGCTTCCTGTACGGGGTCTTCAATGGC
  TATGATGGCAACCGCGTGACCAACTTTGTGGCGCAGAGGCTTTCTGCAGAGCTCCTGCTGGGCCAGCTCA
  ACACTGAGCACACTGAGGCTGATGTGCGACGGGTCCTGCTGCAGGCCTTTGATGTGGTGGAGAGGAGCTT
  CCTGGAGTCTATCGATGATGCCTTAGCTGAGAAAGCAAGCCTCCAGTCCCAGCTGCCGGAGGGTGTGCCC
  CAGCACCAGCTGCCACCTCAGTACCAGAAGATCCTTGAGAGACTCAAGGCACTGGAGAGGGAGATTTCGG
  GAGGAGCCATGGCTGTCGTGGCAGTCCTTCTCAACAGCAAGCTCTACGTTGCCAATGTCGGTACGAACAG
  GGCCCTTCTGTGCAAATCTACAGTGGATGGGTTACAGGTTACACAGCTAAACATGGACCACACCACCGAG
  AACGAGGACGAGCTCTTTCGGCTTTCGCAACTGGGTTTAGACGCAGGGAAGATCAAGCAGATGGGCGTCA
  TCTGTGGACAGGAGAGCACCAGGCGGATTGGGGATTACAAGGTCAAATATGGCTACACCGACATTGACCT
  GCTCAGCGCTGCCAAGTCCAAACCCATCATCGCAGAGCCGGAAATTCATGGCGCACAGCCTCTGGATGGC
  GTGACGGGCTTCCTGGTGCTGATGTCAGAGGGGCTGTACAAGGCCCTGGAGGCAGCCCATGGGCCTGGGC
  AGGCCAACCAGGAGATTGCCGCGATGATTGACACCGAGTTTGCCAAGCAGACCTCCCTGGATGCGGTTGC
  CCAGGCTGTGGTAGACCGTGTAAAGCGGATCCACAGTGACACCTTTGCCAGTGGTGGGGAGCGTGCCAAG
  TTCTGCCCACGGCATGAAGACATGACCCTGCTGGTGAGGAACTTTGGCTATCCATTGGGTGAAATGAGCC
  AGCCTACACCGACCCCTGCCCCAGGGGGCCGTGTGTACCCTGTTTCTGTGCCCTACTCAAGTGCCCAGAG
  CACCAGCAAGACTAGTGTGACTCTGTCCCTCGTCATGCCTTCTCAGGGCCAGATGGTCAACGGCTCTCAC
  AGTGCCTCCACTCTGGACGAAGCCACTCCCACACTCACTAACCAGAGCCCCACTCTGACCCTGCAGTCCA
  CCAACACGCACACCCAGAGCAGCAGCTCCAGCTCTGACGGGGGCCTCTTCCGCTCCAGACCGGCTCACTC
  ACTTCCACCCGGAGAGGATGGCCGTGTGGAGCCCTATGTGGACTTTGCTGAGTTCTACCGACTCTGGAGC
  GTGGACCACGGCGAGCAGAGCGTGATGACGGCACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208071 representing NM_025609
  Red=Cloning site Green=Tags(s)
  
  MAAQRRSLLQSEQQPSWTDDLPLCHLSGVGSASNRSYSADGKGTESHPPEDNWLKFRSENNCFLYGVFNG
  YDGNRVTNFVAQRLSAELLLGQLNTEHTEADVRRVLLQAFDVVERSFLESIDDALAEKASLQSQLPEGVP
  QHQLPPQYQKILERLKALEREISGGAMAVVAVLLNSKLYVANVGTNRALLCKSTVDGLQVTQLNMDHTTE
  NEDELFRLSQLGLDAGKIKQMGVICGQESTRRIGDYKVKYGYTDIDLLSAAKSKPIIAEPEIHGAQPLDG
  VTGFLVLMSEGLYKALEAAHGPGQANQEIAAMIDTEFAKQTSLDAVAQAVVDRVKRIHSDTFASGGERAK
  FCPRHEDMTLLVRNFGYPLGEMSQPTPTPAPGGRVYPVSVPYSSAQSTSKTSVTLSLVMPSQGQMVNGSH
  SASTLDEATPTLTNQSPTLTLQSTNTHTQSSSSSSDGGLFRSRPAHSLPPGEDGRVEPYVDFAEFYRLWS
  VDHGEQSVMTAP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025609
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025609.3
• RefSeq Size: 3013 bp
• RefSeq ORF: 1509 bp
• Locus ID: 66513
• UniProt ID: Q8CF89
• Cytogenetics: 15 E1
• Gene Summary: May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.[UniProtKB/Swiss-Prot Function]