Aldh1a1 (NM_013467) Mouse Tagged ORF Clone

CAT#: MG208039

Aldh1a1 (tGFP-tagged) - Mouse aldehyde dehydrogenase family 1, subfamily A1 (Aldh1a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aldh1a1
• Synonyms: Ahd-2; Ahd2; Aldh1; Aldh1a2; E1; Raldh1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208039 representing NM_013467
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCGCCTGCACAACCTGCAGTCCCTGCCCCACTGGCCGACTTGAAGATTCAACATACCAAGATCT
  TCATAAACAATGAATGGCACAATTCAGTGAGCGGCAAGAAATTTCCAGTTCTTAACCCTGCAACTGAGGA
  GGTCATCTGCCACGTGGAAGAAGGGGACAAGGCTGATGTTGACAAAGCTGTGAAGGCTGCAAGACAGGCT
  TTCCAGATTGGCTCTCCATGGCGCACCATGGATGCTTCAGAGAGGGGCCGCCTGCTGAACAAGCTGGCTG
  ACTTAATGGAGAGAGATCGTCTGCTGCTAGCTACAATGGAGGCACTCAATGGTGGGAAAGTCTTTGCCAA
  TGCATACTTGTCGGATTTAGGAGGCTGCATAAAAGCATTAAAGTACTGTGCAGGCTGGGCTGACAAGATT
  CATGGTCAAACAATACCAAGTGATGGAGACATTTTCACTTATACAAGACGTGAACCTATTGGAGTGTGTG
  GCCAAATCATCCCCTGGAATTTTCCAATGCTCATGTTCATTTGGAAGATAGGCCCTGCCCTTAGCTGTGG
  GAATACCGTGGTTGTCAAGCCAGCAGAGCAAACTCCTCTCACGGCTCTTCACCTGGCATCTTTAATAAAA
  GAGGCAGGGTTTCCTCCTGGCGTGGTAAACATTGTCCCTGGTTATGGGCCAACTGCAGGGGCAGCCATCT
  CCTCTCACATGGATGTCGACAAGGTGGCCTTCACTGGATCAACACAGGTTGGCAAGTTAATCAAGGAAGC
  TGCAGGGAAAAGCAATCTGAAGAGAGTCACCCTGGAGCTTGGGGGAAAGAGCCCTTGCATTGTGTTTGCA
  GATGCCGACTTGGACATTGCTGTTGAGTTTGCACACCATGGAGTGTTTTATCATCAAGGCCAATGCTGTG
  TCGCAGCATCCCGGATTTTTGTTGAGGAGTCAGTTTATGATGAGTTTGTGAAAAGGAGTGTTGAGCGAGC
  TAAGAAATATGTTCTTGGAAATCCTCTGACCCCAGGAATAAATCAAGGCCCTCAGATTGACAAGGAACAA
  CATGATAAAATACTCGATCTCATTGAGAGTGGGAAGAAAGAAGGAGCCAAACTGGAGTGTGGTGGAGGAC
  GCTGGGGGAACAAAGGCTTCTTTGTGCAGCCCACAGTGTTCTCCAACGTGACTGATGAGATGCGCATTGC
  CAAAGAGGAGATATTTGGACCAGTGCAACAAATCATGAAGTTTAAGTCTGTAGATGATGTGATCAAGAGA
  GCAAACAATACTACCTATGGTTTAGCAGCAGGACTCTTCACTAAAGACCTGGATAAGGCCATCACTGTGT
  CATCTGCTCTGCAGGCTGGGGTGGTGTGGGTTAACTGCTATATGATGTTGTCAGCCCAGTGCCCCTTCGG
  TGGATTCAAGATGTCTGGAAATGGAAGAGAACTGGGTGAACATGGTCTTTATGAATACACTGAGCTCAAG
  ACAGTCGCAATGAAGATATCTCAGAAGAACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208039 representing NM_013467
  Red=Cloning site Green=Tags(s)
  
  MSSPAQPAVPAPLADLKIQHTKIFINNEWHNSVSGKKFPVLNPATEEVICHVEEGDKADVDKAVKAARQA
  FQIGSPWRTMDASERGRLLNKLADLMERDRLLLATMEALNGGKVFANAYLSDLGGCIKALKYCAGWADKI
  HGQTIPSDGDIFTYTRREPIGVCGQIIPWNFPMLMFIWKIGPALSCGNTVVVKPAEQTPLTALHLASLIK
  EAGFPPGVVNIVPGYGPTAGAAISSHMDVDKVAFTGSTQVGKLIKEAAGKSNLKRVTLELGGKSPCIVFA
  DADLDIAVEFAHHGVFYHQGQCCVAASRIFVEESVYDEFVKRSVERAKKYVLGNPLTPGINQGPQIDKEQ
  HDKILDLIESGKKEGAKLECGGGRWGNKGFFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSVDDVIKR
  ANNTTYGLAAGLFTKDLDKAITVSSALQAGVVWVNCYMMLSAQCPFGGFKMSGNGRELGEHGLYEYTELK
  TVAMKISQKNS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013467
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013467.2
• RefSeq Size: 2032 bp
• RefSeq ORF: 1506 bp
• Locus ID: 11668
• UniProt ID: P24549
• Cytogenetics: 19 13.91 cM
• Gene Summary: Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (By similarity).[UniProtKB/Swiss-Prot Function]