Angpt2 (NM_007426) Mouse Tagged ORF Clone

CAT#: MG207970

Angpt2 (tGFP-tagged) - Mouse angiopoietin 2 (Angpt2)

Product Images

Circular map for MG207970

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Angpt2
• Synonyms: Agpt2; An; Ang; Ang-2; Ang2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207970 representing NM_007426
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCAGATCATTTTCCTAACTTTTGGCTGGGATCTTGTCTTGGCCTCAGCCTACAGTAACTTTAGGA
  AGAGCGTGGACAGCACAGGCAGAAGGCAGTACCAGGTCCAGAACGGACCCTGCAGCTACACGTTCCTGCT
  GCCGGAGACCGACAGCTGCCGATCTTCCTCCAGCCCCTACATGTCCAATGCCGTGCAGAGGGATGCACCC
  CTCGACTACGACGACTCAGTGCAAAGGCTGCAGGTGCTGGAGAACATTCTAGAGAACAACACACAGTGGC
  TGATGAAGCTGGAGAATTACATTCAGGACAACATGAAGAAGGAGATGGTGGAGATCCAACAGAATGTGGT
  GCAGAACCAGACAGCTGTGATGATAGAGATTGGAACCAGCTTGCTGAACCAGACAGCAGCACAAACTCGG
  AAACTGACTGATGTGGAAGCCCAAGTACTAAACCAGACGACAAGACTCGAGCTGCAGCTTCTCCAACATT
  CTATTTCTACCAACAAATTGGAAAAGCAGATTTTGGATCAGACCAGTGAAATAAACAAGCTACAAAATAA
  GAACAGCTTCCTAGAACAGAAAGTTCTGGACATGGAGGGCAAGCACAGCGAGCAGCTACAGTCCATGAAA
  GAGCAGAAGGACGAGCTCCAGGTGCTGGTGTCCAAGCAGAGCTCTGTCATTGACGAGCTGGAGAAGAAGC
  TGGTGACAGCCACGGTCAACAACTCGCTCCTTCAGAAGCAGCAGCATGACCTAATGGAGACCGTCAACAG
  CTTGCTGACCATGATGTCATCACCCAACTCCAAGAGCTCGGTTGCTATCCGTAAAGAAGAGCAAACCACC
  TTCAGAGACTGTGCGGAAATCTTCAAGTCAGGACTCACCACCAGTGGCATCTACACACTGACCTTCCCCA
  ACTCCACAGAGGAGATCAAGGCCTACTGTGACATGGACGTGGGTGGAGGAGGGTGGACAGTCATCCAACA
  CCGAGAAGATGGCAGTGTGGACTTCCAGAGGACGTGGAAAGAATACAAAGAGGGCTTCGGGAGCCCTCTG
  GGAGAGTACTGGCTGGGCAACGAGTTTGTCTCCCAGCTGACCGGTCAGCACCGCTACGTGCTTAAGATCC
  AGCTGAAGGACTGGGAAGGCAACGAGGCGCATTCGCTGTATGATCACTTCTACCTCGCTGGTGAAGAGTC
  CAACTACAGGATTCACCTTACAGGACTCACGGGGACCGCGGGCAAAATAAGTAGCATCAGCCAACCAGGA
  AGTGATTTTAGCACAAAGGATTCGGACAATGACAAATGCATCTGCAAGTGTTCCCAGATGCTCTCAGGAG
  GCTGGTGGTTTGACGCATGTGGTCCTTCCAACTTGAATGGACAGTACTACCCACAAAAACAGAATACAAA
  TAAGTTTAACGGTATCAAGTGGTACTACTGGAAGGGGTCCGGCTACTCGCTCAAGGCCACAACCATGATG
  ATCCGGCCAGCAGATTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207970 representing NM_007426
  Red=Cloning site Green=Tags(s)
  
  MWQIIFLTFGWDLVLASAYSNFRKSVDSTGRRQYQVQNGPCSYTFLLPETDSCRSSSSPYMSNAVQRDAP
  LDYDDSVQRLQVLENILENNTQWLMKLENYIQDNMKKEMVEIQQNVVQNQTAVMIEIGTSLLNQTAAQTR
  KLTDVEAQVLNQTTRLELQLLQHSISTNKLEKQILDQTSEINKLQNKNSFLEQKVLDMEGKHSEQLQSMK
  EQKDELQVLVSKQSSVIDELEKKLVTATVNNSLLQKQQHDLMETVNSLLTMMSSPNSKSSVAIRKEEQTT
  FRDCAEIFKSGLTTSGIYTLTFPNSTEEIKAYCDMDVGGGGWTVIQHREDGSVDFQRTWKEYKEGFGSPL
  GEYWLGNEFVSQLTGQHRYVLKIQLKDWEGNEAHSLYDHFYLAGEESNYRIHLTGLTGTAGKISSISQPG
  SDFSTKDSDNDKCICKCSQMLSGGWWFDACGPSNLNGQYYPQKQNTNKFNGIKWYYWKGSGYSLKATTMM
  IRPADF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007426
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007426.3, NP_031452.2
• RefSeq Size: 2475 bp
• RefSeq ORF: 1491 bp
• Locus ID: 11601
• UniProt ID: O35608
• Cytogenetics: 8 10.3 cM
• Gene Summary: This gene encodes an endothelial cell (EC)-derived regulator of angiogenesis and ligand for endothelial-specific receptor tyrosine kinase. The encoded protein acts as an anti-apoptotic factor for stressed ECs and a proapoptotic factor for resting ECs. [provided by RefSeq, Jan 2013]