Cd5 (NM_007650) Mouse Tagged ORF Clone

CAT#: MG207937

Cd5 (tGFP-tagged) - Mouse CD5 antigen (Cd5)

Product Images

Circular map for MG207937

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cd5
• Synonyms: Ly-1; Ly-12; Ly-A; Lyt-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207937 representing NM_007650
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCCACGAAGTGCTGCTGGCTGCCACGTACCTGCTGGGAACGCTGGCGGCTTTCTGCCTCGGAC
  AGTCTGGAAGGGGTGGCCTAGATATCCAGGTGATGCTAAGTGGCTCCAACTCCAAGTGTCAGGGTCAAGT
  GGAGATCCAGATGGAAAACAAGTGGAAAACAGTGTGCAGTTCCAGTTGGAGGCTGAGCCAGGACCATTCG
  AAAAATGCCCAGCAGGCCTCTGCAGTGTGCAAAGAGCTGAGATGTGGTGACCCCTTGGCCCTTGGTCCTT
  TCCCTTCATTGAACAGACCCCAGAACCAGGTCTTCTGCCAAGGATCTCCGTGGTCTATATCCAACTGCAA
  CAACACAAGTTCACAAGACCAGTGCCTTCCGCTGAGCCTGATCTGCCTAGAGCCCCAGAGAACGACACCT
  CCACCCACAACCACCCCACCCACCACCGTGCCAGAGCCCACAGCTCCTCCCAGATTGCAGCTGGTGCCAG
  GACACGAAGGCCTGAGGTGCACAGGTGTGGTGGAATTCTACAATGGCAGCTGGGGTGGCACCATCCTCTA
  CAAGGCCAAGGACAGGCCCCTGGGCCTGGGGAACCTCATCTGTAAGTCTCTGCAGTGTGGCTCTTTCTTG
  ACACATCTGTCCGGGACAGAGGCAGCAGGGACACCAGCTCCTGCAGAGCTGAGGGACCCCAGGCCCTTGC
  CAATTCGATGGGAGGCCCCGAACGGGAGCTGTGTCTCACTACAGCAGTGCTTCCAGAAAACAACGGCCCA
  GGAGGGCGGCCAGGCGCTCACCGTCATCTGCTCTGATTTCCAGCCCAAGGTTCAGAGCCGCCTGGTCGGG
  GGCAGCAGTGTGTGTGAGGGCATCGCTGAAGTGCGCCAGAGATCACAGTGGGAGGCCCTGTGTGACAGTT
  CTGCAGCCAGGGGCCGGGGACGGTGGGAAGAGCTATGTCGGGAGCAGCAGTGTGGCGACCTCATCTCCTT
  CCACACGGTGGATGCTGACAAAACCTCCCCCGGGTTCCTCTGTGCCCAGGAGAAGCTGTCTCAGTGTTAC
  CATCTCCAGAAAAAAAAACACTGCAACAAGAGGGTATTCGTCACATGCCAAGACCCAAACCCAGCAGGCC
  TGGCCCCAGGCACTGTGGCAAGCATCATCCTGACCCTTGTACTCCTGGTGGTGCTGCTGGCCATGTGTGG
  TCCTCTGGTCTACAAGAAGCTGGTGAAGAAATTTCGTCAGAAAAAGCAGCGTCAGTGGATTGGCCCCACA
  GGAGTGAACCAGAACATGTCTTTCCATCGAAGCCACACAGCAACTGTGCGGTCCCAAGTTGAGAACCCAA
  CAGCCTCTCATGTGGACAATGAATACAGCCAGCCTCCCAGGAACTCTCACCTGTCTGCTTATCCAGCTCT
  GGAAGGAGCCCTACACCGATCTTCTACACAACCTGACAACTCCTCTGACAGTGACTATGACCTGCAAGTG
  GCTCAGAGACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207937 representing NM_007650
  Red=Cloning site Green=Tags(s)
  
  MDSHEVLLAATYLLGTLAAFCLGQSGRGGLDIQVMLSGSNSKCQGQVEIQMENKWKTVCSSSWRLSQDHS
  KNAQQASAVCKELRCGDPLALGPFPSLNRPQNQVFCQGSPWSISNCNNTSSQDQCLPLSLICLEPQRTTP
  PPTTTPPTTVPEPTAPPRLQLVPGHEGLRCTGVVEFYNGSWGGTILYKAKDRPLGLGNLICKSLQCGSFL
  THLSGTEAAGTPAPAELRDPRPLPIRWEAPNGSCVSLQQCFQKTTAQEGGQALTVICSDFQPKVQSRLVG
  GSSVCEGIAEVRQRSQWEALCDSSAARGRGRWEELCREQQCGDLISFHTVDADKTSPGFLCAQEKLSQCY
  HLQKKKHCNKRVFVTCQDPNPAGLAPGTVASIILTLVLLVVLLAMCGPLVYKKLVKKFRQKKQRQWIGPT
  GVNQNMSFHRSHTATVRSQVENPTASHVDNEYSQPPRNSHLSAYPALEGALHRSSTQPDNSSDSDYDLQV
  AQRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007650
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007650.2
• RefSeq Size: 2069 bp
• RefSeq ORF: 1485 bp
• Locus ID: 12507
• UniProt ID: P13379
• Cytogenetics: 19 7.16 cM
• Gene Summary: May act as a receptor in regulating T-cell proliferation.[UniProtKB/Swiss-Prot Function]