Bin1 (NM_001083334) Mouse Tagged ORF Clone

CAT#: MG207643

Bin1 (tGFP-tagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2

Product Images

Circular map for MG207643

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bin1
• Synonyms: ALP-1; Amphl; BRAMP-2; SH3P9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207643 representing NM_001083334
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGATGGGGAGCAAGGGGGTGACGGCGGGGAAGATCGCCAGCAACGTACAGAAGAAGCTGACCC
  GAGCGCAGGAGAAGGTCCTGCAGAAACTGGGGAAGGCGGACGAGACGAAGGACGAGCAGTTTGAGCAGTG
  TGTCCAGAACTTCAATAAGCAGCTGACAGAGGGTACCCGGCTGCAGAAGGATCTTCGGACCTATCTGGCT
  TCTGTTAAAGCGATGCACGAAGCCTCCAAGAAGCTGAGTGAGTGTCTTCAGGAGGTGTATGAGCCCGAGT
  GGCCTGGCAGGGATGAAGCAAACAAGATTGCAGAGAACAATGACCTACTCTGGATGGACTACCACCAGAA
  GCTGGTGGACCAGGCTCTGCTGACCATGGACACCTACCTAGGCCAGTTCCCTGATATCAAGTCGCGCATT
  GCCAAGCGGGGGCGGAAGCTGGTGGACTATGACAGTGCCCGGCACCACTATGAGTCTCTTCAAACCGCCA
  AAAAGAAGGATGAAGCCAAAATTGCCAAGGCAGAAGAGGAGCTCATCAAAGCCCAGAAGGTGTTCGAGGA
  GATGAACGTGGATCTGCAGGAGGAGCTGCCATCCCTGTGGAACAGCCGTGTAGGTTTCTATGTCAACACG
  TTCCAGAGCATCGCGGGTCTGGAGGAAAACTTCCATAAAGAGATGAGTAAGCTCAATCAGAACCTCAATG
  ATGTCCTGGTCAGCCTAGAGAAGCAGCACGGGAGCAACACCTTCACAGTCAAGGCCCAACCCAGTGACAA
  TGCCCCTGAGAAAGGGAACAAGAGCCCGTCACCTCCTCCAGATGGCTCCCCTGCTGCTACCCCTGAGATC
  AGAGTGAACCATGAGCCAGAGCCGGCCAGTGGGGCCTCACCCGGGGCTACCATCCCCAAGTCCCCATCTC
  AGCTCCGGAAAGGCCCACCTGTCCCTCCGCCTCCCAAACACACCCCATCCAAGGAGATGAAGCAGGAGCA
  GATTCTCAGCCTTTTTGATGACGCATTTGTCCCTGAGATCAGCGTGACCACCCCCTCCCAGCCAGCAGAG
  GCCTCCGAGGTGGTGGGTGGAGCCCAGGAGCCAGGGGAGACAGCAGCCAGTGAAGCAACCTCCAGCTCTC
  TTCCGGCTGTGGTGGTGGAGACCTTCTCCGCAACTGTGAATGGGGCGGTGGAGGGCAGCGCTGGGACTGG
  ACGCTTGGACCTGCCCCCGGGATTCATGTTCAAGGTTCAAGCCCAGCATGATTACACGGCCACTGACACT
  GATGAGCTGCAACTCAAAGCTGGCGATGTGGTGTTGGTGATTCCTTTCCAGAACCCAGAGGAGCAGGATG
  AAGGCTGGCTCATGGGTGTGAAGGAGAGCGACTGGAATCAGCACAAGGAACTGGAGAAATGCCGCGGCGT
  CTTCCCGGAGAATTTTACAGAGCGGGTGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207643 representing NM_001083334
  Red=Cloning site Green=Tags(s)
  
  MAEMGSKGVTAGKIASNVQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTRLQKDLRTYLA
  SVKAMHEASKKLSECLQEVYEPEWPGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRI
  AKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKAEEELIKAQKVFEEMNVDLQEELPSLWNSRVGFYVNT
  FQSIAGLEENFHKEMSKLNQNLNDVLVSLEKQHGSNTFTVKAQPSDNAPEKGNKSPSPPPDGSPAATPEI
  RVNHEPEPASGASPGATIPKSPSQLRKGPPVPPPPKHTPSKEMKQEQILSLFDDAFVPEISVTTPSQPAE
  ASEVVGGAQEPGETAASEATSSSLPAVVVETFSATVNGAVEGSAGTGRLDLPPGFMFKVQAQHDYTATDT
  DELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001083334
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083334.1, NP_001076803.1
• RefSeq Size: 2135 bp
• RefSeq ORF: 1434 bp
• Locus ID: 30948
• Cytogenetics: 18 18.01 cM
• Gene Summary: Is a key player in the control of plasma membrane curvature, and membrane shaping and remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:12183633). Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:12668730, PubMed:27179792). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation (By similarity).[UniProtKB/Swiss-Prot Function]