Snx17 (NM_153680) Mouse Tagged ORF Clone

CAT#: MG207515

Snx17 (tGFP-tagged) - Mouse sorting nexin 17 (Snx17)

Product Images

Circular map for MG207515

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Snx17
• Synonyms: 5830447M19Rik; AI790646; b2b1625.1Clo; D5Ertd260e; mKIAA0064
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207515 representing NM_153680
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTTTTCCATTCCTGAAACGGAGTCCCGCAGTGGGGACAGCGGCGGCTCCGCCTACGTGGCCTATA
  ACATTCACGTGAATGGAGTCCTGCACTGTCGGGTGCGCTACAGCCAGCTTCTGGGGCTGCACGAGCAGCT
  TCGGAAGGAGTATGGGGCCAACGTGGTTCCTGCATTCCCCCCAAAGAAGCTGTTCTCTTTGACCCCTGCA
  GAGGTGGAGCAGAGGAGAGAGCAGTTGGAGAAGTACATGCAAGCTGTTCGGCAAGACCCATTGCTTGGGA
  GCAGTGAGACTTTTAATAGCTTTCTACGTCGGGCCCAACAGGAGACCCAACAGGTGCCCACAGAGGAGGT
  TTCCTTGGAAGTGCTGCTCAGCAATGGACAGAAAGTTCTGGTAACTGTGCTAACTTCTGACCAGACAGAA
  GATGTCCTGGAGGCTGTGGCTGCAAAGCTGGATCTTCCAGATGACTTGATCGGATATTTTAGTCTCTTCC
  TTGTTCGAGAAAAAGAAGATGGAGCCTTTTCTTTTGTACGGAAGCTGCAGGAGTTTGAACTGCCTTATGT
  ATCTGTTACCAGTCTTCGGAGTCAAGAGTATAAGATTGTGCTCAGAAAGAGTTATTGGGACTCTGCCTAT
  GACGACGATGTCATGGAGAACCGGGTTGGCCTGAACCTGCTTTATGCTCAGACGGTGTCAGACATTGAGC
  ATGGCTGGATCCTGGTCACCAAAGAGCAGCACCGGCAGCTCAAATCTCTGCAAGAGAAGGTCTCCAAAAA
  GGAGTTCTTGCGGCTGGCCCAAACTCTGCGGCACTATGGCTATCTGCGCTTTGATGCCTGTGTGGCTGAC
  TTTCCAGAGAAGGATTGCCCTGTTGTGGTGAGCGCAGGCAACAGTGAGCTCAGTCTCCAGCTGCGACTAC
  CAGGCCAGCAACTCCGTGAAGGCTCTTTCCGTGTTACCCGAATGCGATGCTGGCGGGTCACCTCCTCTGT
  GCCACTCCCTAGTGGGGGCACGAGCAGTCCAAGCCGGGGTCGGGGTGAGGTTCGCCTGGAACTGGCTTTT
  GAATACCTCATGAGCAAGGACCGGCTACAGTGGGTCACCATCACCAGCCCACAGGCTATCATGATGAGCA
  TCTGCCTGCAGTCCATGGTAGATGAGCTGATGGTGAAGAAATCTGGGGGCAGTATCAGGAAGATGCTGCG
  CCGGCGGGTGGGGGGCACCCTGAGACGTTCAGACAGCCAGCAAGCAGTGAAGTCCCCACCATTGCTCGAG
  TCACCAGACGCCAGCCGGGAGTCCATGGTGAAACTCTCAAGTAAACTGAGTGCTGTGAGCTTGCGGGGGA
  TTGGCAGTCCCAGCACAGATGCCAGTGCCAGTGCCGTCCACGGCAATTTCGCCTTCGAGGGCATTGGAGA
  TGAGGATCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207515 representing NM_153680
  Red=Cloning site Green=Tags(s)
  
  MHFSIPETESRSGDSGGSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVVPAFPPKKLFSLTPA
  EVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVTVLTSDQTE
  DVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAY
  DDDVMENRVGLNLLYAQTVSDIEHGWILVTKEQHRQLKSLQEKVSKKEFLRLAQTLRHYGYLRFDACVAD
  FPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGGTSSPSRGRGEVRLELAF
  EYLMSKDRLQWVTITSPQAIMMSICLQSMVDELMVKKSGGSIRKMLRRRVGGTLRRSDSQQAVKSPPLLE
  SPDASRESMVKLSSKLSAVSLRGIGSPSTDASASAVHGNFAFEGIGDEDL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153680
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153680.1
• RefSeq Size: 1941 bp
• RefSeq ORF: 1413 bp
• Locus ID: 266781
• UniProt ID: Q8BVL3
• Cytogenetics: 5 B1
• Gene Summary: Critical regulator of endosomal recycling of numerous surface proteins, including integrins, signaling receptor and channels (PubMed:12169628, PubMed:16052210). Binds to NPxY sequences in the cytoplasmic tails of target cargos (By similarity). Associates with retriever and CCC complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGB1, ITGB5 and their associated alpha subunits (By similarity). Also required for maintenance of normal cell surface levels of APP and LRP1 (PubMed:16052210, PubMed:18276590). Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) (By similarity).[UniProtKB/Swiss-Prot Function]