Irf6 (NM_016851) Mouse Tagged ORF Clone

CAT#: MG207464

  • TrueORF®

Irf6 (tGFP-tagged) - Mouse interferon regulatory factor 6 (Irf6)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_016851" in other vectors (6)

Reconstitution Protocol

USD 886.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Irf6
Synonyms AI876454; E230028I05Rik; mirf6
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207464 representing NM_016851
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTCCACCCTCGAAGAGTCCGGCTGAAGCCCTGGCTGGTGGCCCAGGTGGACAGTGGCCTCTACC
CTGGTCTCATCTGGCTACACAGAGATTCCAAACGCTTCCAGATCCCCTGGAAACATGCCACGCGGCACAG
CCCCCAACAAGAGGAAGAAAACACCATTTTTAAGGCTTGGGCTGTGGAGACCGGAAAGTACCAGGAAGGG
GTAGACGATCCTGACCCAGCTAAATGGAAGGCTCAGCTCCGCTGTGCTCTCAACAAAAGCAGGGAGTTCA
ACTTGATGTACGATGGCACCAAGGAAGTGCCCATGAATCCTGTGAAGATCTATCAAGTGTGTGACATCCC
CCAGACCCAGGGCTCTGTCATTAATCCAGGATCCACAGGGTCTGCTCCTTGGGATGAGAAAGATAATGAT
GTGGATGAAGATGAGGAGGAGGATGAACTTGAGCAGTCACAGCACCATGTCCCCATCCAGGACACCTTCC
CCTTCCTGAACATCAACGGTTCTCCCATGGCGCCAGCCAGCGTGGGCAACTGCAGTGTGGGAAACTGCAG
CCCCGAATCAGTGTGGCCCAAAACAGAACCTCTGGAGATGGAAGTACCCCAGGCACCCATTCAACCCTTC
TACAGTTCTCCAGAGCTATGGATCAGCTCTCTCCCCATGACTGACTTGGACATCAAATTTCAGTATCGTG
GGAAGGAGTATGGGCAAACTATGACGGTGAGCAACCCCCAGGGCTGCCGGCTCTTCTATGGGGACCTGGG
CCCCATGCCCGACCAGGAGGAGCTCTTTGGTCCTGTCAGCCTGGAGCAGGTCAAGTTCCCAGGTCCAGAG
CATATCACCAACGAGAAGCAGAAGCTGTTCACCAGCAAGCTGTTGGATGTCATGGACAGGGGACTGATCC
TGGAGGTCAGCGGACATGCCATTTATGCCATCAGGCTGTGCCAGTGCAAAGTGTACTGGTCAGGGCCATG
TGCTCCCTCGCTTGCTGCTCCTAACCTGATTGAGAGGCAGAAGAAGGTCAAGTTGTTTTGTCTGGAAACC
TTCCTGAGTGAGCTCATTGCCCACCAGAAAGGACAGATAGAGAAGCAGCCGCCTTTTGAGATCTACTTAT
GCTTTGGGGAAGAGTGGCCAGATGGAAAGCCCCTGGAGAGGAAGCTCATCTTGGTCCAGGTCATCCCAGT
GGTGGCTCGCATGATCTATGAGATGTTTTCTGGTGATTTTACCCGGTCCTTTGACAGTGGCAGCGTTCGC
CTGCAGATCTCCACTCCAGACATCAAAGATAACATCGTTGCTCAGCTCAAGCAGCTGTACCGCATCCTTC
AAACCCAGGAGAGCTGGCAGCCCATGCAGCCTGCCCCCAGCATGCAGCTGCCACAGGCTCTGCCTGCCCA
G


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207464 representing NM_016851
Red=Cloning site Green=Tags(s)

MALHPRRVRLKPWLVAQVDSGLYPGLIWLHRDSKRFQIPWKHATRHSPQQEEENTIFKAWAVETGKYQEG
VDDPDPAKWKAQLRCALNKSREFNLMYDGTKEVPMNPVKIYQVCDIPQTQGSVINPGSTGSAPWDEKDND
VDEDEEEDELEQSQHHVPIQDTFPFLNINGSPMAPASVGNCSVGNCSPESVWPKTEPLEMEVPQAPIQPF
YSSPELWISSLPMTDLDIKFQYRGKEYGQTMTVSNPQGCRLFYGDLGPMPDQEELFGPVSLEQVKFPGPE
HITNEKQKLFTSKLLDVMDRGLILEVSGHAIYAIRLCQCKVYWSGPCAPSLAAPNLIERQKKVKLFCLET
FLSELIAHQKGQIEKQPPFEIYLCFGEEWPDGKPLERKLILVQVIPVVARMIYEMFSGDFTRSFDSGSVR
LQISTPDIKDNIVAQLKQLYRILQTQESWQPMQPAPSMQLPQALPAQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016851
ORF Size 1401 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016851.2, NP_058547.2
RefSeq Size 4140 bp
RefSeq ORF 1404 bp
Locus ID 54139
UniProt ID P97431
Cytogenetics 1 97.6 cM
Gene Summary Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development. Plays a role in regulating mammary epithelial cell proliferation. May regulate WDR65 transcription.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.