Ndufs2 (NM_153064) Mouse Tagged ORF Clone

CAT#: MG207399

Ndufs2 (tGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) Fe-S protein 2 (Ndufs2)

Product Images

Circular map for MG207399

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ndufs2
• Synonyms: CI-49kD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207399 representing NM_153064
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTGAGGGCGCTGCGCTGCCTCCGTGGGGTCGGAGCCCCGGTGCTGCGGCCCGGGTCTGGGA
  TCCGCTTGCCAAGTCAGCCCAGCAGAGGTGCTCGGCAGTGGCAGCCAGATATTGAATGGGCAGAGCAGTT
  TTCGGGAGCTGTCATGTACCCCTCCAAGGAAACAGCCCACTGGAAACCTCCTCCTTGGAATGATGTGGAC
  ATTTTGAAGGAAAAAGCGGTGACCAACATGACCCTGAACTTTGGGCCCCAGCACCCAGCAGCCCACGGAG
  TCCTGAGACTCGTGCTGGAACTGAGTGGAGAGATGGTGCGGAAATGTGACCCTCACATCGGGCTCCTGCA
  CCGAGGCACGGAGAAGCTCATTGAGTACAAGACCTATCTGCAGGCCCTTCCATACTTTGACCGGTTGGAC
  TATGTGTCCATGATGTGTAATGAACAGGCCTATTCGATAGCTGTGGAGAAGTTGCTAAACATCCAACCTC
  CTCCTCGGGCACAGTGGATCCGAGTGCTCTTTGGAGAGATCACACGGATTTTAAACCATATCATGGCTGT
  CACCACACATGCCCTGGACATCGGTGCCATGACTCCTTTCTTCTGGATGTTTGAAGAAAGAGAGAAGATG
  TTCGAGTTCTATGAGCGGGTGTCTGGGGCCCGGATGCACGCGGCGTATATCCGACCTGGAGGAGTGCACC
  AGGACCTACCTCTTGGGCTTCTGGATGACATTTATGAGTTTTCCAAGAACTTCTCTCTTCGGATTGATGA
  GGTGGAGGAGATGCTGACCAACAATAGAATCTGGCGAAATAGGACAGTCGACATTGGGGTTGTAACAGCG
  GAAGACGCACTTAACTATGGATTCAGTGGGGTGATGCTCCGAGGCTCAGGCATCCAGTGGGACTTGCGGA
  AGACCCAGCCCTATGACGTTTACGACCAGGTGGAGTTTGATGTTCCTATCGGTTCTCGAGGGGACTGCTA
  CGATAGGTACCTGTGTCGTGTGGAGGAGATGCGCCAGTCCCTGCGAATCATTGAGCAGTGTCTGAACAAG
  ATGCCTCCCGGGGAGATCAAGGTTGATGACGCCAAAGTGTCCCCACCGAAGCGAGCAGAGATGAAGACGT
  CCATGGAGTCACTAATTCATCACTTTAAGCTGTATACAGAGGGCTACCAAGTTCCTCCAGGAGCCACATA
  TACTGCCATTGAAGCTCCTAAGGGAGAGTTTGGGGTATACTTGGTATCCGATGGCAGCAGCCGCCCTTAT
  CGGTGTAAGATCAAGGCTCCCGGTTTTGCCCACCTGGCTGGTTTGGACAAGATGTCTAAGGGACACATGT
  TGGCAGATGTCGTGGCCATCATAGGTACCCAGGATATTGTGTTCGGAGAAATAGACCGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207399 representing NM_153064
  Red=Cloning site Green=Tags(s)
  
  MAALRALRCLRGVGAPVLRPGSGIRLPSQPSRGARQWQPDIEWAEQFSGAVMYPSKETAHWKPPPWNDVD
  ILKEKAVTNMTLNFGPQHPAAHGVLRLVLELSGEMVRKCDPHIGLLHRGTEKLIEYKTYLQALPYFDRLD
  YVSMMCNEQAYSIAVEKLLNIQPPPRAQWIRVLFGEITRILNHIMAVTTHALDIGAMTPFFWMFEEREKM
  FEFYERVSGARMHAAYIRPGGVHQDLPLGLLDDIYEFSKNFSLRIDEVEEMLTNNRIWRNRTVDIGVVTA
  EDALNYGFSGVMLRGSGIQWDLRKTQPYDVYDQVEFDVPIGSRGDCYDRYLCRVEEMRQSLRIIEQCLNK
  MPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAIEAPKGEFGVYLVSDGSSRPY
  RCKIKAPGFAHLAGLDKMSKGHMLADVVAIIGTQDIVFGEIDR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153064
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153064.5, NP_694704.1
• RefSeq Size: 1606 bp
• RefSeq ORF: 1392 bp
• Locus ID: 226646
• UniProt ID: Q91WD5
• Cytogenetics: 1 H3
• Gene Summary: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.[UniProtKB/Swiss-Prot Function]