Eef1a1 (NM_010106) Mouse Tagged ORF Clone

CAT#: MG207381

Eef1a1 (tGFP-tagged) - Mouse eukaryotic translation elongation factor 1 alpha 1 (Eef1a1)

Product Images

Circular map for MG207381

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eef1a1
• Synonyms: MGC7551; MGC8115; MGC8209; MGC18758; MGC27859; MGC102592; MGC103271; MGC118397
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207381 representing NM_010106
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAGGAAAAGACTCACATCAACATCGTCGTAATCGGACACGTAGATTCCGGCAAGTCCACCACAA
  CCGGCCACCTGATCTACAAATGTGGTGGAATCGACAAGCGAACCATCGAAAAGTTTGAGAAAGAGGCTGC
  TGAGATGGGAAAGGGCTCCTTCAAGTACGCCTGGGTCTTAGACAAACTGAAAGCTGAGCGTGAGCGTGGT
  ATCACTATTGACATCTCCCTGTGGAAATTCGAGACCAGCAAATACTATGTGACCATCATTGATGCCCCAG
  GACACAGAGACTTCATCAAAAACATGATTACAGGCACATCCCAGGCTGACTGTGCTGTCCTGATTGTTGC
  TGCTGGTGTTGGTGAATTTGAAGCTGGTATCTCCAAGAACGGGCAGACCCGCGAGCATGCTCTTCTGGCT
  TACACCCTGGGTGTGAAACAGCTGATTGTTGGTGTCAACAAAATGGATTCCACCGAGCCACCATACAGTC
  AGAAGAGATACGAGGAAATCGTTAAGGAAGTCAGCACCTACATTAAGAAAATTGGCTACAACCCTGACAC
  AGTAGCATTTGTGCCAATTTCTGGTTGGAATGGTGACAACATGCTGGAGCCAAGTGCTAATATGCCTTGG
  TTCAAGGGATGGAAAGTCACCCGCAAAGATGGCAGTGCCAGTGGCACCACGCTGCTGGAAGCTTTGGATT
  GTATCCTACCACCAACTCGTCCAACTGACAAGCCTCTGCGACTGCCCCTCCAGGATGTCTATAAAATTGG
  AGGCATTGGCACTGTCCCTGTGGGCCGAGTGGAGACTGGTGTTCTCAAACCTGGCATGGTGGTTACCTTT
  GCTCCAGTCAATGTAACAACTGAAGTCAAGTCTGTTGAAATGCACCATGAAGCTTTGAGTGAAGCTCTTC
  CTGGGGACAATGTGGGCTTCAATGTAAAGAACGTGTCGGTCAAAGATGTTAGACGAGGCAATGTTGCTGG
  TGACAGCAAAAACGACCCACCAATGGAAGCAGCTGGCTTCACTGCTCAGGTGATTATCCTGAACCATCCA
  GGCCAAATCAGTGCTGGCTACGCTCCTGTTCTGGATTGTCACACAGCCCACATAGCATGCAAGTTTGCTG
  AGCTTAAAGAAAAGATCGATCGTCGTTCTGGTAAGAAGCTGGAAGATGGCCCCAAGTTCCTGAAGTCTGG
  CGATGCTGCCATTGTTGATATGGTCCCTGGCAAGCCCATGTGTGTTGAGAGCTTCTCTGACTACCCTCCA
  CTTGGTCGCTTTGCTGTTCGTGACATGAGGCAGACAGTTGCTGTGGGTGTCATCAAAGCTGTGGACAAGA
  AGGCTGCTGGAGCTGGCAAAGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207381 representing NM_010106
  Red=Cloning site Green=Tags(s)
  
  MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERG
  ITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLA
  YTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPW
  FKGWKVTRKDGSASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF
  APVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHP
  GQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPP
  LGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010106
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010106.2, NP_034236.2
• RefSeq Size: 1790 bp
• RefSeq ORF: 1389 bp
• Locus ID: 13627
• UniProt ID: P10126
• Cytogenetics: 9 E1
• Gene Summary: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.[UniProtKB/Swiss-Prot Function]