Tat (NM_146214) Mouse Tagged ORF Clone

CAT#: MG207246

Tat (tGFP-tagged) - Mouse tyrosine aminotransferase (Tat)

Product Images

Circular map for MG207246

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tat
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207246 representing NM_146214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCTACGTAATCCAGACGAATGTCAATGACAGCCTACCTTCAGTGCTGGATGTTCGCGTCAATA
  TTGGCGGGAGAAGCTCGGTGCAAGGGAGAGCGAAGGGCAGGAAGGCCAGGTGGAATGTGAGGCCCTCTGA
  CATGTCCAATAAAACCTTCAATCCCATCCGAGCCATTGTGGACAACATGAAGGTGAAGCCGAATCCGAAC
  AAAACCGTGATCTCTCTGTCAATTGGGGACCCTACTGTGTTTGGGAACCTGCCCACAGACCCTGAAGTGA
  CCCAAGCCATGAAGGATGCCCTGGACTCCGGGAAGTACAATGGCTATGCCCCATCTATCGGCTACCTATC
  CAGTCGGGAGGAGGTCGCTTCCTATTACCACTGTCCTGAGGCTCCTCTGGAAGCTAAGGATGTCATTCTG
  ACGAGTGGCTGCAGTCAGGCCATTGAGCTGTGTCTAGCCGTGTTGGCCAATCCTGGACAGAACATCCTCA
  TTCCGAGGCCCGGGTTTTCCCTCTACAGGACATTGGCTGAGTCTATGGGGATTGAGGTCAAGCTCTACAA
  TCTATTGCCTGAGAAGTCTTGGGAAATTGATCTAAAACAACTGGAATCTCTGATCGACGAAAAAACAGCT
  TGTCTCGTGGTCAACAACCCGTCCAATCCCTGTGGCTCTGTGTTCAGTAAGCGGCACCTTCAGAAGATTT
  TGGCAGTGGCTGAAAGGCAATGCGTCCCCATCTTAGCCGATGAGATCTATGGTGACATGGTGTTTTCAGA
  TTGCAAATATGAACCAATGGCCACCCTCAGCACCAATGTCCCCATCCTGTCCTGTGGTGGGCTGGCCAAG
  CGCTGGCTGGTTCCTGGCTGGAGGCTGGGCTGGATCCTTATCCATGATCGAAGAGACATTTTTGGCAATG
  AGATTCGGGACGGGCTGGTGAAGCTGAGTCAGCGGATCCTGGGCCCGTGCACCATCGTCCAAGGTGCCCT
  GAAGAGCATCCTTCAGCGCACCCCTCAGGAGTTCTACCAGGACACTTTAAGCTTCCTTAAGTCCAATGCG
  GACCTCTGCTATGGGGCGTTGTCTGCCATTCCTGGACTCCAGCCAGTCCGCCCATCTGGAGCCATGTACC
  TTATGGTGGGAATTGAGATGGAGCACTTCCCAGAATTTGAGAATGACGTGGAATTCACAGAGCGGTTAAT
  TGCGGAGCAGTCTGTCCACTGCCTCCCAGCCACGTGCTTCGAGTACCCAAATTTCTTCCGGGTTGTCATC
  ACAGTCCCCGAGGTGATGATGCTGGAGGCTTGTAGCCGGATCCAGGAGTTCTGTGAACAGCACTACCACT
  GTGCTGAAGGCAGCCAGGAGGAGTGTGACAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207246 representing NM_146214
  Red=Cloning site Green=Tags(s)
  
  MDSYVIQTNVNDSLPSVLDVRVNIGGRSSVQGRAKGRKARWNVRPSDMSNKTFNPIRAIVDNMKVKPNPN
  KTVISLSIGDPTVFGNLPTDPEVTQAMKDALDSGKYNGYAPSIGYLSSREEVASYYHCPEAPLEAKDVIL
  TSGCSQAIELCLAVLANPGQNILIPRPGFSLYRTLAESMGIEVKLYNLLPEKSWEIDLKQLESLIDEKTA
  CLVVNNPSNPCGSVFSKRHLQKILAVAERQCVPILADEIYGDMVFSDCKYEPMATLSTNVPILSCGGLAK
  RWLVPGWRLGWILIHDRRDIFGNEIRDGLVKLSQRILGPCTIVQGALKSILQRTPQEFYQDTLSFLKSNA
  DLCYGALSAIPGLQPVRPSGAMYLMVGIEMEHFPEFENDVEFTERLIAEQSVHCLPATCFEYPNFFRVVI
  TVPEVMMLEACSRIQEFCEQHYHCAEGSQEECDK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146214
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146214.3
• RefSeq Size: 2353 bp
• RefSeq ORF: 1365 bp
• Locus ID: 234724
• UniProt ID: Q8QZR1
• Cytogenetics: 8 57.38 cM
• Gene Summary: This gene encodes a liver-specific mitochondrial enzyme that catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Regulated by glucocorticoid and polypeptide hormones, this gene's expression is affected by deletion of a regulatory region near the albino locus on chromosome 7. Mutations in this gene cause tyrosinemia type II in humans. [provided by RefSeq, Mar 2010]