Tubb6 (NM_026473) Mouse Tagged ORF Clone

CAT#: MG207133

Tubb6 (tGFP-tagged) - Mouse tubulin, beta 6 (Tubb6)

Product Images

Circular map for MG207133

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tubb6
• Synonyms: 2310057H16Rik; BB220206
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207133 representing NM_026473
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGAGATTGTGCACATCCAGGCGGGCCAGTGCGGGAACCAGATCGGTACCAAGTTTTGGGAAGTGA
  TCAGTGATGAGCACGGCATCGACCAGGCCGGAGGCTACGTGGGCGACTCAGCGCTGCAGCTGGAGAGGAT
  CAGCGTCTACTACAATGAGTCATCCTCTAAGAAGTACGTACCCAGGGCTGCCCTGGTGGACTTGGAGCCC
  GGCACCATGGACAGTGTGAGGTCTGGGCCTTTTGGGCAACTCTTCCGGCCTGACAACTTCATCTTCGGAC
  AGACGGGTGCCGGAAACAACTGGGCCAAGGGTCACTACACGGAGGGCGCGGAGCTGGTGGATTCGGTGCT
  GGATGTGGTGCGCAAGGAGTGTGAGCATTGCGACTGTCTTCAGGGCTTCCAGCTCACCCACTCGCTGGGC
  GGTGGCACGGGCTCAGGCATGGGCACACTGCTCATCAGCAAGATCCGAGAGGAGTACCCGGACCGCATCA
  TGAACACCTTCAGCGTCATGCCGTCACCCAAGGTCTCAGACACCGTGGTGGAGCCCTACAACGCCACATT
  GTCAGTGCACCAGCTGGTAGAGAACACCGACGAGACCTACTGCATCGACAACGAGGCCCTCTATGACATC
  TGCTTCCGCACGCTCAAGCTGACCACACCCACTTACGGGGACCTCAACCACTTGGTATCCGCCACCATGA
  GCGGTGTCACCACATCACTGCGTTTCCCTGGCCAACTCAACGCCGACCTGCGCAAGCTGGCTGTGAACAT
  GGTGCCATTCCCACGTCTCCACTTCTTCATGCCCGGCTTTGCCCCCCTCACAGCCCGGGGCAGTCAGCAG
  TACCGTGCCCTGACAGTGCCTGAGCTCACACAGCAGATGTTCGATGCCAAGAACATGATGGCTGCCTGTG
  ACCCACGCCATGGCCGCTACCTGACCGTCGCCACTGTCTTCCGGGGCCCCATGTCCATGAAGGAGGTGGA
  CGAGCAGATGCTGGCCATCCAGAACAAGAACAGCAGCTACTTTGTGGAGTGGATCCCCAACAATGTCAAG
  GTAGCTGTGTGCGACATCCCACCACGGGGCCTGAAGATGGCCTCCACCTTCATTGGCAATAGCACTGCCA
  TCCAGGAGCTATTCAAGCGCATCTCCGAGCAGTTCTCGGCCATGTTCCGCCGCAAGGCCTTCCTGCACTG
  GTTCACCGGTGAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGTAACATGAATGACCTGGTGTCC
  GAGTACCAGCAGTACCAGGACGCCACGGTCAATGATGGGGAAGAGGCATTTGAAGACGAGGATGAAGAAG
  AGATCAACGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207133 representing NM_026473
  Red=Cloning site Green=Tags(s)
  
  MREIVHIQAGQCGNQIGTKFWEVISDEHGIDQAGGYVGDSALQLERISVYYNESSSKKYVPRAALVDLEP
  GTMDSVRSGPFGQLFRPDNFIFGQTGAGNNWAKGHYTEGAELVDSVLDVVRKECEHCDCLQGFQLTHSLG
  GGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDI
  CFRTLKLTTPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQ
  YRALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGPMSMKEVDEQMLAIQNKNSSYFVEWIPNNVK
  VAVCDIPPRGLKMASTFIGNSTAIQELFKRISEQFSAMFRRKAFLHWFTGEGMDEMEFTEAESNMNDLVS
  EYQQYQDATVNDGEEAFEDEDEEEINE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026473
• ORF Size: 1341 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026473.2, NP_080749.2
• RefSeq Size: 1807 bp
• RefSeq ORF: 1344 bp
• Locus ID: 67951
• UniProt ID: Q922F4
• Cytogenetics: 18 E1
• Gene Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[UniProtKB/Swiss-Prot Function]